Cargando…

Intramuscular inflammatory and resolving lipid profile responses to an acute bout of resistance exercise in men

Lipid mediators including classical arachidonic acid‐derived eicosanoids (e.g. prostaglandins and leukotrienes) and more recently identified specialized pro‐resolving‐mediator metabolites of the omega‐3 fatty acids play essential roles in initiation, self‐limitation, and active resolution of acute i...

Descripción completa

Detalles Bibliográficos
Autores principales: Vella, Luke, Markworth, James F., Farnfield, Michelle M., Maddipati, Krishna R., Russell, Aaron P., Cameron‐Smith, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6599756/
https://www.ncbi.nlm.nih.gov/pubmed/31257737
http://dx.doi.org/10.14814/phy2.14108
_version_ 1783430989126565888
author Vella, Luke
Markworth, James F.
Farnfield, Michelle M.
Maddipati, Krishna R.
Russell, Aaron P.
Cameron‐Smith, David
author_facet Vella, Luke
Markworth, James F.
Farnfield, Michelle M.
Maddipati, Krishna R.
Russell, Aaron P.
Cameron‐Smith, David
author_sort Vella, Luke
collection PubMed
description Lipid mediators including classical arachidonic acid‐derived eicosanoids (e.g. prostaglandins and leukotrienes) and more recently identified specialized pro‐resolving‐mediator metabolites of the omega‐3 fatty acids play essential roles in initiation, self‐limitation, and active resolution of acute inflammatory responses. In this study, we examined the bioactive lipid mediator profile of human skeletal muscle at rest and following acute resistance exercise. Twelve male subjects completed a single bout of maximal isokinetic unilateral knee extension exercise and muscle biopsies were taken from the m.vastus lateralis before and at 2, 4, and 24 h of recovery. Muscle tissue lipid mediator profile was analyzed via liquid chromatography–mass spectrometry (LC‐MS)‐based targeted lipidomics. At 2 h postexercise, there was an increased intramuscular abundance of cyclooxygenase (COX)‐derived thromboxanes (TXB (2): 3.33 fold) and prostaglandins (PGE (2): 2.52 fold and PGF (2α): 1.77 fold). Resistance exercise also transiently increased muscle concentrations of lipoxygenase (LOX) pathway‐derived leukotrienes (12‐Oxo LTB (4): 1.49 fold and 20‐COOH LTB (4): 2.91 fold), monohydroxy‐eicosatetraenoic acids (5‐HETE: 2.66 fold, 12‐HETE: 2.83 fold, and 15‐HETE: 1.69 fold) and monohydroxy‐docosahexaenoic acids (4‐HDoHE: 1.69 fold, 7‐HDoHE: 1.58 fold and 14‐HDoHE: 2.35 fold). Furthermore, the abundance of CYP pathway‐derived epoxy‐ and dihydroxy‐eicosatrienoic acids was increased in 2 h postexercise biopsies (5,6‐EpETrE: 2.48 fold, 11,12‐DiHETrE: 1.66 fold and 14,15‐DiHETrE: 2.23 fold). These data reveal a range of bioactive lipid mediators as present within human skeletal muscle tissue and demonstrate that acute resistance exercise transiently stimulates the local production of both proinflammatory eicosanoids and pathway markers in specialized proresolving mediator biosynthesis circuits.
format Online
Article
Text
id pubmed-6599756
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-65997562019-07-12 Intramuscular inflammatory and resolving lipid profile responses to an acute bout of resistance exercise in men Vella, Luke Markworth, James F. Farnfield, Michelle M. Maddipati, Krishna R. Russell, Aaron P. Cameron‐Smith, David Physiol Rep Original Research Lipid mediators including classical arachidonic acid‐derived eicosanoids (e.g. prostaglandins and leukotrienes) and more recently identified specialized pro‐resolving‐mediator metabolites of the omega‐3 fatty acids play essential roles in initiation, self‐limitation, and active resolution of acute inflammatory responses. In this study, we examined the bioactive lipid mediator profile of human skeletal muscle at rest and following acute resistance exercise. Twelve male subjects completed a single bout of maximal isokinetic unilateral knee extension exercise and muscle biopsies were taken from the m.vastus lateralis before and at 2, 4, and 24 h of recovery. Muscle tissue lipid mediator profile was analyzed via liquid chromatography–mass spectrometry (LC‐MS)‐based targeted lipidomics. At 2 h postexercise, there was an increased intramuscular abundance of cyclooxygenase (COX)‐derived thromboxanes (TXB (2): 3.33 fold) and prostaglandins (PGE (2): 2.52 fold and PGF (2α): 1.77 fold). Resistance exercise also transiently increased muscle concentrations of lipoxygenase (LOX) pathway‐derived leukotrienes (12‐Oxo LTB (4): 1.49 fold and 20‐COOH LTB (4): 2.91 fold), monohydroxy‐eicosatetraenoic acids (5‐HETE: 2.66 fold, 12‐HETE: 2.83 fold, and 15‐HETE: 1.69 fold) and monohydroxy‐docosahexaenoic acids (4‐HDoHE: 1.69 fold, 7‐HDoHE: 1.58 fold and 14‐HDoHE: 2.35 fold). Furthermore, the abundance of CYP pathway‐derived epoxy‐ and dihydroxy‐eicosatrienoic acids was increased in 2 h postexercise biopsies (5,6‐EpETrE: 2.48 fold, 11,12‐DiHETrE: 1.66 fold and 14,15‐DiHETrE: 2.23 fold). These data reveal a range of bioactive lipid mediators as present within human skeletal muscle tissue and demonstrate that acute resistance exercise transiently stimulates the local production of both proinflammatory eicosanoids and pathway markers in specialized proresolving mediator biosynthesis circuits. John Wiley and Sons Inc. 2019-06-30 /pmc/articles/PMC6599756/ /pubmed/31257737 http://dx.doi.org/10.14814/phy2.14108 Text en © 2019 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Vella, Luke
Markworth, James F.
Farnfield, Michelle M.
Maddipati, Krishna R.
Russell, Aaron P.
Cameron‐Smith, David
Intramuscular inflammatory and resolving lipid profile responses to an acute bout of resistance exercise in men
title Intramuscular inflammatory and resolving lipid profile responses to an acute bout of resistance exercise in men
title_full Intramuscular inflammatory and resolving lipid profile responses to an acute bout of resistance exercise in men
title_fullStr Intramuscular inflammatory and resolving lipid profile responses to an acute bout of resistance exercise in men
title_full_unstemmed Intramuscular inflammatory and resolving lipid profile responses to an acute bout of resistance exercise in men
title_short Intramuscular inflammatory and resolving lipid profile responses to an acute bout of resistance exercise in men
title_sort intramuscular inflammatory and resolving lipid profile responses to an acute bout of resistance exercise in men
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6599756/
https://www.ncbi.nlm.nih.gov/pubmed/31257737
http://dx.doi.org/10.14814/phy2.14108
work_keys_str_mv AT vellaluke intramuscularinflammatoryandresolvinglipidprofileresponsestoanacuteboutofresistanceexerciseinmen
AT markworthjamesf intramuscularinflammatoryandresolvinglipidprofileresponsestoanacuteboutofresistanceexerciseinmen
AT farnfieldmichellem intramuscularinflammatoryandresolvinglipidprofileresponsestoanacuteboutofresistanceexerciseinmen
AT maddipatikrishnar intramuscularinflammatoryandresolvinglipidprofileresponsestoanacuteboutofresistanceexerciseinmen
AT russellaaronp intramuscularinflammatoryandresolvinglipidprofileresponsestoanacuteboutofresistanceexerciseinmen
AT cameronsmithdavid intramuscularinflammatoryandresolvinglipidprofileresponsestoanacuteboutofresistanceexerciseinmen