Development and Optimization of Expression, Purification, and ATPase Assay of KaiC for Medium-Throughput Screening of Circadian Clock Mutants in Cyanobacteria

The slow but temperature-insensitive adenosine triphosphate (ATP) hydrolysis reaction in KaiC is considered as one of the factors determining the temperature-compensated period length of the cyanobacterial circadian clock system. Structural units responsible for this low but temperature-compensated...

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Detalles Bibliográficos
Autores principales: Ouyang, Dongyan, Furuike, Yoshihiko, Mukaiyama, Atsushi, Ito-Miwa, Kumiko, Kondo, Takao, Akiyama, Shuji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600144/
https://www.ncbi.nlm.nih.gov/pubmed/31181593
http://dx.doi.org/10.3390/ijms20112789

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600144/
https://www.ncbi.nlm.nih.gov/pubmed/31181593
http://dx.doi.org/10.3390/ijms20112789

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