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Identification of Potential Novel Interacting Partners for Coagulation Factor XIII B (FXIII-B) Subunit, a Protein Associated with a Rare Bleeding Disorder

Coagulation factor XIII (FXIII) is a plasma-circulating heterotetrameric pro-transglutaminase complex that is composed of two catalytic FXIII-A and two protective/regulatory FXIII-B subunits. FXIII acts by forming covalent cross-links within a preformed fibrin clots to prevent its premature fibrinol...

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Autores principales: Singh, Sneha, Akhter, Mohammad Suhail, Dodt, Johannes, Volkers, Peter, Reuter, Andreas, Reinhart, Christoph, Krettler, Christoph, Oldenburg, Johannes, Biswas, Arijit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600159/
https://www.ncbi.nlm.nih.gov/pubmed/31159152
http://dx.doi.org/10.3390/ijms20112682
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author Singh, Sneha
Akhter, Mohammad Suhail
Dodt, Johannes
Volkers, Peter
Reuter, Andreas
Reinhart, Christoph
Krettler, Christoph
Oldenburg, Johannes
Biswas, Arijit
author_facet Singh, Sneha
Akhter, Mohammad Suhail
Dodt, Johannes
Volkers, Peter
Reuter, Andreas
Reinhart, Christoph
Krettler, Christoph
Oldenburg, Johannes
Biswas, Arijit
author_sort Singh, Sneha
collection PubMed
description Coagulation factor XIII (FXIII) is a plasma-circulating heterotetrameric pro-transglutaminase complex that is composed of two catalytic FXIII-A and two protective/regulatory FXIII-B subunits. FXIII acts by forming covalent cross-links within a preformed fibrin clots to prevent its premature fibrinolysis. The FXIII-A subunit is known to have pleiotropic roles outside coagulation, but the FXIII-B subunit is a relatively unexplored entity, both structurally as well as functionally. Its discovered roles so far are limited to that of the carrier/regulatory protein of its partner FXIII-A subunit. In the present study, we have explored the co-presence of protein excipients in commercial FXIII plasma concentrate FibrogamminP by combination of protein purification and mass spectrometry-based verification. Complement factor H was one of the co-excipients observed in this analysis. This was followed by performing pull down assays from plasma in order to detect the putative novel interacting partners for the FXIII-B subunit. Complement system proteins, like complement C3 and complement C1q, were amongst the proteins that were pulled down. The only protein that was observed in both experimental set ups was alpha-2-macroglobulin, which might therefore be a putative interacting partner of the FXIII/FXIII-B subunit. Future functional investigations will be needed to understand the physiological significance of this association.
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spelling pubmed-66001592019-07-16 Identification of Potential Novel Interacting Partners for Coagulation Factor XIII B (FXIII-B) Subunit, a Protein Associated with a Rare Bleeding Disorder Singh, Sneha Akhter, Mohammad Suhail Dodt, Johannes Volkers, Peter Reuter, Andreas Reinhart, Christoph Krettler, Christoph Oldenburg, Johannes Biswas, Arijit Int J Mol Sci Article Coagulation factor XIII (FXIII) is a plasma-circulating heterotetrameric pro-transglutaminase complex that is composed of two catalytic FXIII-A and two protective/regulatory FXIII-B subunits. FXIII acts by forming covalent cross-links within a preformed fibrin clots to prevent its premature fibrinolysis. The FXIII-A subunit is known to have pleiotropic roles outside coagulation, but the FXIII-B subunit is a relatively unexplored entity, both structurally as well as functionally. Its discovered roles so far are limited to that of the carrier/regulatory protein of its partner FXIII-A subunit. In the present study, we have explored the co-presence of protein excipients in commercial FXIII plasma concentrate FibrogamminP by combination of protein purification and mass spectrometry-based verification. Complement factor H was one of the co-excipients observed in this analysis. This was followed by performing pull down assays from plasma in order to detect the putative novel interacting partners for the FXIII-B subunit. Complement system proteins, like complement C3 and complement C1q, were amongst the proteins that were pulled down. The only protein that was observed in both experimental set ups was alpha-2-macroglobulin, which might therefore be a putative interacting partner of the FXIII/FXIII-B subunit. Future functional investigations will be needed to understand the physiological significance of this association. MDPI 2019-05-31 /pmc/articles/PMC6600159/ /pubmed/31159152 http://dx.doi.org/10.3390/ijms20112682 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Singh, Sneha
Akhter, Mohammad Suhail
Dodt, Johannes
Volkers, Peter
Reuter, Andreas
Reinhart, Christoph
Krettler, Christoph
Oldenburg, Johannes
Biswas, Arijit
Identification of Potential Novel Interacting Partners for Coagulation Factor XIII B (FXIII-B) Subunit, a Protein Associated with a Rare Bleeding Disorder
title Identification of Potential Novel Interacting Partners for Coagulation Factor XIII B (FXIII-B) Subunit, a Protein Associated with a Rare Bleeding Disorder
title_full Identification of Potential Novel Interacting Partners for Coagulation Factor XIII B (FXIII-B) Subunit, a Protein Associated with a Rare Bleeding Disorder
title_fullStr Identification of Potential Novel Interacting Partners for Coagulation Factor XIII B (FXIII-B) Subunit, a Protein Associated with a Rare Bleeding Disorder
title_full_unstemmed Identification of Potential Novel Interacting Partners for Coagulation Factor XIII B (FXIII-B) Subunit, a Protein Associated with a Rare Bleeding Disorder
title_short Identification of Potential Novel Interacting Partners for Coagulation Factor XIII B (FXIII-B) Subunit, a Protein Associated with a Rare Bleeding Disorder
title_sort identification of potential novel interacting partners for coagulation factor xiii b (fxiii-b) subunit, a protein associated with a rare bleeding disorder
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600159/
https://www.ncbi.nlm.nih.gov/pubmed/31159152
http://dx.doi.org/10.3390/ijms20112682
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