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Anti-Candida Biofilm Activity of Pterostilbene or Crude Extract from Non-Fermented Grape Pomace Entrapped in Biopolymeric Nanoparticles

Polymeric nanoparticle-based carriers are promising agents to deliver drugs to cells. Vitis vinifera phenolic compounds are known for their antifungal activity against Candida albicans. The aim of the present study was to investigate the antifungal activity of pterostilbene or crude extracts from no...

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Autores principales: Simonetti, Giovanna, Palocci, Cleofe, Valletta, Alessio, Kolesova, Olga, Chronopoulou, Laura, Donati, Livia, Di Nitto, Antonio, Brasili, Elisa, Tomai, Pierpaolo, Gentili, Alessandra, Pasqua, Gabriella
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600237/
https://www.ncbi.nlm.nih.gov/pubmed/31151290
http://dx.doi.org/10.3390/molecules24112070
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author Simonetti, Giovanna
Palocci, Cleofe
Valletta, Alessio
Kolesova, Olga
Chronopoulou, Laura
Donati, Livia
Di Nitto, Antonio
Brasili, Elisa
Tomai, Pierpaolo
Gentili, Alessandra
Pasqua, Gabriella
author_facet Simonetti, Giovanna
Palocci, Cleofe
Valletta, Alessio
Kolesova, Olga
Chronopoulou, Laura
Donati, Livia
Di Nitto, Antonio
Brasili, Elisa
Tomai, Pierpaolo
Gentili, Alessandra
Pasqua, Gabriella
author_sort Simonetti, Giovanna
collection PubMed
description Polymeric nanoparticle-based carriers are promising agents to deliver drugs to cells. Vitis vinifera phenolic compounds are known for their antifungal activity against Candida albicans. The aim of the present study was to investigate the antifungal activity of pterostilbene or crude extracts from non-fermented grape pomace, entrapped in poly(lactic-co-glycolic) acid nanoparticles (NPs), with diameters of 50 and 150 nm, on Candida biofilm. The fluorescent probe coumarin 6 was used to study the uptake of poly(lactic-co-glycolic)acid (PLGA) NPs in planktonic cells and biofilm. The green fluorescent signal of coumarin 6 was observed in Candida biofilm after 24 and 48 hours. Both pterostilbene and crude pomace extract entrapped in NPs exerted a significantly higher anti-biofilm activity compared to their free forms. The entrapment efficiency of both pterostilbene and crude pomace extract in PLGA NPs was ~90%. At 16 µg/mL, pterostilbene loaded in PLGA NPs reduced biofilm formation of 63% and reduced mature biofilm of 50%. Moreover, at 50 µg/mL, the pomace extract loaded in NPs reduced mature biofilm of 37%. These results strongly suggest that PLGA NPs are promising nanodevices for the delivery of antifungal drugs as the crude grape pomace extract, a by-product of white wine making.
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spelling pubmed-66002372019-07-16 Anti-Candida Biofilm Activity of Pterostilbene or Crude Extract from Non-Fermented Grape Pomace Entrapped in Biopolymeric Nanoparticles Simonetti, Giovanna Palocci, Cleofe Valletta, Alessio Kolesova, Olga Chronopoulou, Laura Donati, Livia Di Nitto, Antonio Brasili, Elisa Tomai, Pierpaolo Gentili, Alessandra Pasqua, Gabriella Molecules Article Polymeric nanoparticle-based carriers are promising agents to deliver drugs to cells. Vitis vinifera phenolic compounds are known for their antifungal activity against Candida albicans. The aim of the present study was to investigate the antifungal activity of pterostilbene or crude extracts from non-fermented grape pomace, entrapped in poly(lactic-co-glycolic) acid nanoparticles (NPs), with diameters of 50 and 150 nm, on Candida biofilm. The fluorescent probe coumarin 6 was used to study the uptake of poly(lactic-co-glycolic)acid (PLGA) NPs in planktonic cells and biofilm. The green fluorescent signal of coumarin 6 was observed in Candida biofilm after 24 and 48 hours. Both pterostilbene and crude pomace extract entrapped in NPs exerted a significantly higher anti-biofilm activity compared to their free forms. The entrapment efficiency of both pterostilbene and crude pomace extract in PLGA NPs was ~90%. At 16 µg/mL, pterostilbene loaded in PLGA NPs reduced biofilm formation of 63% and reduced mature biofilm of 50%. Moreover, at 50 µg/mL, the pomace extract loaded in NPs reduced mature biofilm of 37%. These results strongly suggest that PLGA NPs are promising nanodevices for the delivery of antifungal drugs as the crude grape pomace extract, a by-product of white wine making. MDPI 2019-05-30 /pmc/articles/PMC6600237/ /pubmed/31151290 http://dx.doi.org/10.3390/molecules24112070 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Simonetti, Giovanna
Palocci, Cleofe
Valletta, Alessio
Kolesova, Olga
Chronopoulou, Laura
Donati, Livia
Di Nitto, Antonio
Brasili, Elisa
Tomai, Pierpaolo
Gentili, Alessandra
Pasqua, Gabriella
Anti-Candida Biofilm Activity of Pterostilbene or Crude Extract from Non-Fermented Grape Pomace Entrapped in Biopolymeric Nanoparticles
title Anti-Candida Biofilm Activity of Pterostilbene or Crude Extract from Non-Fermented Grape Pomace Entrapped in Biopolymeric Nanoparticles
title_full Anti-Candida Biofilm Activity of Pterostilbene or Crude Extract from Non-Fermented Grape Pomace Entrapped in Biopolymeric Nanoparticles
title_fullStr Anti-Candida Biofilm Activity of Pterostilbene or Crude Extract from Non-Fermented Grape Pomace Entrapped in Biopolymeric Nanoparticles
title_full_unstemmed Anti-Candida Biofilm Activity of Pterostilbene or Crude Extract from Non-Fermented Grape Pomace Entrapped in Biopolymeric Nanoparticles
title_short Anti-Candida Biofilm Activity of Pterostilbene or Crude Extract from Non-Fermented Grape Pomace Entrapped in Biopolymeric Nanoparticles
title_sort anti-candida biofilm activity of pterostilbene or crude extract from non-fermented grape pomace entrapped in biopolymeric nanoparticles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600237/
https://www.ncbi.nlm.nih.gov/pubmed/31151290
http://dx.doi.org/10.3390/molecules24112070
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