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Production of Stilbenes in Callus Cultures of the Maltese Indigenous Grapevine Variety, Ġellewża
The production of secondary metabolites in tissue culture has been considered as an alternative to the cultivation and harvesting of crops intended for this purpose. The present study was aimed at the growth of callus and production of polyphenolic compound of callus derived from a Maltese indigenou...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600261/ https://www.ncbi.nlm.nih.gov/pubmed/31167390 http://dx.doi.org/10.3390/molecules24112112 |
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author | Bonello, Mariella Gašić, Uroš Tešić, Živoslav Attard, Everaldo |
author_facet | Bonello, Mariella Gašić, Uroš Tešić, Živoslav Attard, Everaldo |
author_sort | Bonello, Mariella |
collection | PubMed |
description | The production of secondary metabolites in tissue culture has been considered as an alternative to the cultivation and harvesting of crops intended for this purpose. The present study was aimed at the growth of callus and production of polyphenolic compound of callus derived from a Maltese indigenous grapevine variety, Ġellewża. Callus was inoculated onto plant growth regulators-enriched Murashige Skoog media (MSm) to determine whether polyphenols are produced in vitro as well as to determine the best combination of plant growth regulators needed for the production of these metabolites. From results obtained, it was observed that the best callus production was obtained by auxin-enriched MSm. In fact, indole acetic acid and indole acetic acid /6-benzyl aminopurine enhanced biomass accumulation (3.04 g and 3.39 g) as opposed to the others (<1.97 g). On the other hand, parameters showing the presence of flavonoids (tonality, 3.80), particularly anthocyanins (24.09 mg/kg) and total polyphenols (1.42 mg/g), were optimum in the presence of cytokinins, particularly 6-benzyl aminopurine. Analysis for single polyphenols revealed a high amount a particular stilbene: polydatin (glucoside of resveratrol). Resveratrol and other typical polyphenols, found in mature berries, were also found in significant quantities, while the other polyphenolic compounds were found in minimal quantities. This is the first study to describe the production and composition of polyphenols in Ġellewża callus cultures. From the results obtained, it can be seen that this grape tissue is an excellent alternative for the production of polyphenols from the stilbene group, which can be upscaled and exploited commercially. |
format | Online Article Text |
id | pubmed-6600261 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-66002612019-07-16 Production of Stilbenes in Callus Cultures of the Maltese Indigenous Grapevine Variety, Ġellewża Bonello, Mariella Gašić, Uroš Tešić, Živoslav Attard, Everaldo Molecules Article The production of secondary metabolites in tissue culture has been considered as an alternative to the cultivation and harvesting of crops intended for this purpose. The present study was aimed at the growth of callus and production of polyphenolic compound of callus derived from a Maltese indigenous grapevine variety, Ġellewża. Callus was inoculated onto plant growth regulators-enriched Murashige Skoog media (MSm) to determine whether polyphenols are produced in vitro as well as to determine the best combination of plant growth regulators needed for the production of these metabolites. From results obtained, it was observed that the best callus production was obtained by auxin-enriched MSm. In fact, indole acetic acid and indole acetic acid /6-benzyl aminopurine enhanced biomass accumulation (3.04 g and 3.39 g) as opposed to the others (<1.97 g). On the other hand, parameters showing the presence of flavonoids (tonality, 3.80), particularly anthocyanins (24.09 mg/kg) and total polyphenols (1.42 mg/g), were optimum in the presence of cytokinins, particularly 6-benzyl aminopurine. Analysis for single polyphenols revealed a high amount a particular stilbene: polydatin (glucoside of resveratrol). Resveratrol and other typical polyphenols, found in mature berries, were also found in significant quantities, while the other polyphenolic compounds were found in minimal quantities. This is the first study to describe the production and composition of polyphenols in Ġellewża callus cultures. From the results obtained, it can be seen that this grape tissue is an excellent alternative for the production of polyphenols from the stilbene group, which can be upscaled and exploited commercially. MDPI 2019-06-04 /pmc/articles/PMC6600261/ /pubmed/31167390 http://dx.doi.org/10.3390/molecules24112112 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bonello, Mariella Gašić, Uroš Tešić, Živoslav Attard, Everaldo Production of Stilbenes in Callus Cultures of the Maltese Indigenous Grapevine Variety, Ġellewża |
title | Production of Stilbenes in Callus Cultures of the Maltese Indigenous Grapevine Variety, Ġellewża |
title_full | Production of Stilbenes in Callus Cultures of the Maltese Indigenous Grapevine Variety, Ġellewża |
title_fullStr | Production of Stilbenes in Callus Cultures of the Maltese Indigenous Grapevine Variety, Ġellewża |
title_full_unstemmed | Production of Stilbenes in Callus Cultures of the Maltese Indigenous Grapevine Variety, Ġellewża |
title_short | Production of Stilbenes in Callus Cultures of the Maltese Indigenous Grapevine Variety, Ġellewża |
title_sort | production of stilbenes in callus cultures of the maltese indigenous grapevine variety, ġellewża |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600261/ https://www.ncbi.nlm.nih.gov/pubmed/31167390 http://dx.doi.org/10.3390/molecules24112112 |
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