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Identification of Proteins Differentially Expressed by Adipose-derived Mesenchymal Stem Cells Isolated from Immunodeficient Mice

Although cell therapy using adipose-derived mesenchymal stem cells (AdMSCs) regulates immunity, the degree to which cell quality and function are affected by differences in immunodeficiency of donors is unknown. We used liquid chromatography tandem-mass spectrometry (LC MS/MS) to identify the protei...

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Autores principales: Nakashima, Yoshiki, Nahar, Saifun, Miyagi-Shiohira, Chika, Kinjo, Takao, Kobayashi, Naoya, Kitamura, Shinji, Saitoh, Issei, Watanabe, Masami, Fujita, Jiro, Noguchi, Hirofumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600271/
https://www.ncbi.nlm.nih.gov/pubmed/31151297
http://dx.doi.org/10.3390/ijms20112672
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author Nakashima, Yoshiki
Nahar, Saifun
Miyagi-Shiohira, Chika
Kinjo, Takao
Kobayashi, Naoya
Kitamura, Shinji
Saitoh, Issei
Watanabe, Masami
Fujita, Jiro
Noguchi, Hirofumi
author_facet Nakashima, Yoshiki
Nahar, Saifun
Miyagi-Shiohira, Chika
Kinjo, Takao
Kobayashi, Naoya
Kitamura, Shinji
Saitoh, Issei
Watanabe, Masami
Fujita, Jiro
Noguchi, Hirofumi
author_sort Nakashima, Yoshiki
collection PubMed
description Although cell therapy using adipose-derived mesenchymal stem cells (AdMSCs) regulates immunity, the degree to which cell quality and function are affected by differences in immunodeficiency of donors is unknown. We used liquid chromatography tandem-mass spectrometry (LC MS/MS) to identify the proteins expressed by mouse AdMSCs (mAsMSCs) isolated from normal (C57BL/6) mice and mice with severe combined immunodeficiency (SCID). The protein expression profiles of each strain were 98%–100% identical, indicating that the expression levels of major proteins potentially associated with the therapeutic effects of mAdMSCs were highly similar. Further, comparable levels of cell surface markers (CD44, CD90.2) were detected using flow cytometry or LC MS/MS. MYH9, ACTN1, CANX, GPI, TPM1, EPRS, ITGB1, ANXA3, CNN2, MAPK1, PSME2, CTPS1, OTUB1, PSMB6, HMGB1, RPS19, SEC61A1, CTNNB1, GLO1, RPL22, PSMA2, SYNCRIP, PRDX3, SAMHD1, TCAF2, MAPK3, RPS24, and MYO1E, which are associated with immunity, were expressed at higher levels by the SCID mAdMSCs compared with the C57BL/6 mAdMSCs. In contrast, ANXA9, PCBP2, LGALS3, PPP1R14B, and PSMA6, which are also associated with immunity, were more highly expressed by C57BL/6 mAdMSCs than SCID mAdMSCs. These findings implicate these two sets of proteins in the pathogenesis and maintenance of immunodeficiency.
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spelling pubmed-66002712019-07-16 Identification of Proteins Differentially Expressed by Adipose-derived Mesenchymal Stem Cells Isolated from Immunodeficient Mice Nakashima, Yoshiki Nahar, Saifun Miyagi-Shiohira, Chika Kinjo, Takao Kobayashi, Naoya Kitamura, Shinji Saitoh, Issei Watanabe, Masami Fujita, Jiro Noguchi, Hirofumi Int J Mol Sci Article Although cell therapy using adipose-derived mesenchymal stem cells (AdMSCs) regulates immunity, the degree to which cell quality and function are affected by differences in immunodeficiency of donors is unknown. We used liquid chromatography tandem-mass spectrometry (LC MS/MS) to identify the proteins expressed by mouse AdMSCs (mAsMSCs) isolated from normal (C57BL/6) mice and mice with severe combined immunodeficiency (SCID). The protein expression profiles of each strain were 98%–100% identical, indicating that the expression levels of major proteins potentially associated with the therapeutic effects of mAdMSCs were highly similar. Further, comparable levels of cell surface markers (CD44, CD90.2) were detected using flow cytometry or LC MS/MS. MYH9, ACTN1, CANX, GPI, TPM1, EPRS, ITGB1, ANXA3, CNN2, MAPK1, PSME2, CTPS1, OTUB1, PSMB6, HMGB1, RPS19, SEC61A1, CTNNB1, GLO1, RPL22, PSMA2, SYNCRIP, PRDX3, SAMHD1, TCAF2, MAPK3, RPS24, and MYO1E, which are associated with immunity, were expressed at higher levels by the SCID mAdMSCs compared with the C57BL/6 mAdMSCs. In contrast, ANXA9, PCBP2, LGALS3, PPP1R14B, and PSMA6, which are also associated with immunity, were more highly expressed by C57BL/6 mAdMSCs than SCID mAdMSCs. These findings implicate these two sets of proteins in the pathogenesis and maintenance of immunodeficiency. MDPI 2019-05-30 /pmc/articles/PMC6600271/ /pubmed/31151297 http://dx.doi.org/10.3390/ijms20112672 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nakashima, Yoshiki
Nahar, Saifun
Miyagi-Shiohira, Chika
Kinjo, Takao
Kobayashi, Naoya
Kitamura, Shinji
Saitoh, Issei
Watanabe, Masami
Fujita, Jiro
Noguchi, Hirofumi
Identification of Proteins Differentially Expressed by Adipose-derived Mesenchymal Stem Cells Isolated from Immunodeficient Mice
title Identification of Proteins Differentially Expressed by Adipose-derived Mesenchymal Stem Cells Isolated from Immunodeficient Mice
title_full Identification of Proteins Differentially Expressed by Adipose-derived Mesenchymal Stem Cells Isolated from Immunodeficient Mice
title_fullStr Identification of Proteins Differentially Expressed by Adipose-derived Mesenchymal Stem Cells Isolated from Immunodeficient Mice
title_full_unstemmed Identification of Proteins Differentially Expressed by Adipose-derived Mesenchymal Stem Cells Isolated from Immunodeficient Mice
title_short Identification of Proteins Differentially Expressed by Adipose-derived Mesenchymal Stem Cells Isolated from Immunodeficient Mice
title_sort identification of proteins differentially expressed by adipose-derived mesenchymal stem cells isolated from immunodeficient mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600271/
https://www.ncbi.nlm.nih.gov/pubmed/31151297
http://dx.doi.org/10.3390/ijms20112672
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