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Influence of Magnesium Ions on the Preparation and Storage of DNA Tetrahedrons in Micromolar Ranges
The DNA tetrahedron (Td), as one of the novel DNA-based nanoscale biomaterials, has been extensively studied because of its excellent biocompatibility and increased possibilities for decorating precisely. Although the use of Td in laboratories is well established, knowledge surrounding the factors i...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600315/ https://www.ncbi.nlm.nih.gov/pubmed/31159358 http://dx.doi.org/10.3390/molecules24112091 |
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author | Hu, Yue Chen, Zhou Hou, Zheng Li, Mingkai Ma, Bo Luo, Xiaoxing Xue, Xiaoyan |
author_facet | Hu, Yue Chen, Zhou Hou, Zheng Li, Mingkai Ma, Bo Luo, Xiaoxing Xue, Xiaoyan |
author_sort | Hu, Yue |
collection | PubMed |
description | The DNA tetrahedron (Td), as one of the novel DNA-based nanoscale biomaterials, has been extensively studied because of its excellent biocompatibility and increased possibilities for decorating precisely. Although the use of Td in laboratories is well established, knowledge surrounding the factors influencing its preparation and storage is lacking. In this research, we investigated the role of the magnesium ions, which greatly affect the structure and stability of DNA. We assembled 1, 2, 5, 10 and 20 μM Td in buffers containing different Mg(2+) concentrations, demonstrating that 2 and 5 mM Mg(2+) is optimal in these conditions, and that yields decrease dramatically once the DNA concentration reaches 20 μM or the Mg(2+) concentration is lower than 0.5 mM. We also verified that the Td structure is retained better through freeze-thawing than lyophilization. Furthermore, a lower initial Mg(2+) (≤2 mM) benefited the maintenance of Td structure in the process of lyophilization. Hence, our research sheds light on the influence of Mg(2+) in the process of preparing and storing Td, and also provides some enlightenment on improving yields of other DNA nanostructures. |
format | Online Article Text |
id | pubmed-6600315 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-66003152019-07-16 Influence of Magnesium Ions on the Preparation and Storage of DNA Tetrahedrons in Micromolar Ranges Hu, Yue Chen, Zhou Hou, Zheng Li, Mingkai Ma, Bo Luo, Xiaoxing Xue, Xiaoyan Molecules Article The DNA tetrahedron (Td), as one of the novel DNA-based nanoscale biomaterials, has been extensively studied because of its excellent biocompatibility and increased possibilities for decorating precisely. Although the use of Td in laboratories is well established, knowledge surrounding the factors influencing its preparation and storage is lacking. In this research, we investigated the role of the magnesium ions, which greatly affect the structure and stability of DNA. We assembled 1, 2, 5, 10 and 20 μM Td in buffers containing different Mg(2+) concentrations, demonstrating that 2 and 5 mM Mg(2+) is optimal in these conditions, and that yields decrease dramatically once the DNA concentration reaches 20 μM or the Mg(2+) concentration is lower than 0.5 mM. We also verified that the Td structure is retained better through freeze-thawing than lyophilization. Furthermore, a lower initial Mg(2+) (≤2 mM) benefited the maintenance of Td structure in the process of lyophilization. Hence, our research sheds light on the influence of Mg(2+) in the process of preparing and storing Td, and also provides some enlightenment on improving yields of other DNA nanostructures. MDPI 2019-06-01 /pmc/articles/PMC6600315/ /pubmed/31159358 http://dx.doi.org/10.3390/molecules24112091 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hu, Yue Chen, Zhou Hou, Zheng Li, Mingkai Ma, Bo Luo, Xiaoxing Xue, Xiaoyan Influence of Magnesium Ions on the Preparation and Storage of DNA Tetrahedrons in Micromolar Ranges |
title | Influence of Magnesium Ions on the Preparation and Storage of DNA Tetrahedrons in Micromolar Ranges |
title_full | Influence of Magnesium Ions on the Preparation and Storage of DNA Tetrahedrons in Micromolar Ranges |
title_fullStr | Influence of Magnesium Ions on the Preparation and Storage of DNA Tetrahedrons in Micromolar Ranges |
title_full_unstemmed | Influence of Magnesium Ions on the Preparation and Storage of DNA Tetrahedrons in Micromolar Ranges |
title_short | Influence of Magnesium Ions on the Preparation and Storage of DNA Tetrahedrons in Micromolar Ranges |
title_sort | influence of magnesium ions on the preparation and storage of dna tetrahedrons in micromolar ranges |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600315/ https://www.ncbi.nlm.nih.gov/pubmed/31159358 http://dx.doi.org/10.3390/molecules24112091 |
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