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Microspheres of alginate encapsulated minocycline-loaded nanocrystalline carbonated hydroxyapatite: therapeutic potential and effects on bone regeneration

Background and objective: Tetracycline and its derivatives, combined with calcium phosphates, have been proposed as a delivery system to control inflammatory processes and chronic infections. The objective of this study was to evaluate the microspheres of alginate encapsulated minocycline-loaded nan...

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Autores principales: Calasans-Maia, Mônica Diuana, Barboza Junior, Carlos Alberto Brazil, Soriano-Souza, Carlos Alberto, Alves, Adriana Terezinha Neves Novellino, Uzeda, Marcelo Jose de Pinheiro, Martinez-Zelaya, Victor R, Mavropoulos, Elena, Rocha Leão, Maria Helena, de Santana, Ronaldo Barcellos, Granjeiro, Jose Mauro, Rossi, Alexandre Malta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600321/
https://www.ncbi.nlm.nih.gov/pubmed/31417258
http://dx.doi.org/10.2147/IJN.S201631
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author Calasans-Maia, Mônica Diuana
Barboza Junior, Carlos Alberto Brazil
Soriano-Souza, Carlos Alberto
Alves, Adriana Terezinha Neves Novellino
Uzeda, Marcelo Jose de Pinheiro
Martinez-Zelaya, Victor R
Mavropoulos, Elena
Rocha Leão, Maria Helena
de Santana, Ronaldo Barcellos
Granjeiro, Jose Mauro
Rossi, Alexandre Malta
author_facet Calasans-Maia, Mônica Diuana
Barboza Junior, Carlos Alberto Brazil
Soriano-Souza, Carlos Alberto
Alves, Adriana Terezinha Neves Novellino
Uzeda, Marcelo Jose de Pinheiro
Martinez-Zelaya, Victor R
Mavropoulos, Elena
Rocha Leão, Maria Helena
de Santana, Ronaldo Barcellos
Granjeiro, Jose Mauro
Rossi, Alexandre Malta
author_sort Calasans-Maia, Mônica Diuana
collection PubMed
description Background and objective: Tetracycline and its derivatives, combined with calcium phosphates, have been proposed as a delivery system to control inflammatory processes and chronic infections. The objective of this study was to evaluate the microspheres of alginate encapsulated minocycline-loaded nanocrystalline carbonated hydroxyapatite (CHAMINO) as a biomimetic device to carry out target-controlled drug delivery for alveolar bone repair. Methods: CHAMINO microspheres were implanted in a rat central incisor socket after 7 and 42 days. New bone was formed in both groups between 7 and 42 days of implantation. However, the bone growth was significantly higher for the CHAMINO microspheres. Results: The minocycline (MINO) loading capacity of the nanocrystaline carbonated hydroxyapatite (CHA) nanoparticles was 25.1±2.2 µg MINO/mg CHA for adsorption over 24 hrs. The alginate microspheres containing minocycline-loaded CHA were biologically active and inhibited the Enterococcus faecalis culture growth for up to seven days of the MINO release. An osteoblastic cell viability assay based on the resazurin reduction was conducted after the cells were exposed to the CHAMINO powder and CHAMINO microspheres. Thus, it was found that the alginate extracts encapsulated the minocycline-loaded CHA microspheres and did not affect the osteoblastic cell viability, while the minocycline-doped CHA powder reduced the cell viability by 90%. Conclusion: This study concluded that the alginate microspheres encapsulating the minocycline-loaded nanocrystalline carbonated hydroxyapatite exhibited combined antibacterial activity against Enterococcus faecalis with cytocompatibility and osteoconduction properties. The significant improvement in the new bone formation after 42 days of implantation suggests that the CHAMINO microsphere has potential in clinical applications of bone regeneration.
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spelling pubmed-66003212019-08-15 Microspheres of alginate encapsulated minocycline-loaded nanocrystalline carbonated hydroxyapatite: therapeutic potential and effects on bone regeneration Calasans-Maia, Mônica Diuana Barboza Junior, Carlos Alberto Brazil Soriano-Souza, Carlos Alberto Alves, Adriana Terezinha Neves Novellino Uzeda, Marcelo Jose de Pinheiro Martinez-Zelaya, Victor R Mavropoulos, Elena Rocha Leão, Maria Helena de Santana, Ronaldo Barcellos Granjeiro, Jose Mauro Rossi, Alexandre Malta Int J Nanomedicine Original Research Background and objective: Tetracycline and its derivatives, combined with calcium phosphates, have been proposed as a delivery system to control inflammatory processes and chronic infections. The objective of this study was to evaluate the microspheres of alginate encapsulated minocycline-loaded nanocrystalline carbonated hydroxyapatite (CHAMINO) as a biomimetic device to carry out target-controlled drug delivery for alveolar bone repair. Methods: CHAMINO microspheres were implanted in a rat central incisor socket after 7 and 42 days. New bone was formed in both groups between 7 and 42 days of implantation. However, the bone growth was significantly higher for the CHAMINO microspheres. Results: The minocycline (MINO) loading capacity of the nanocrystaline carbonated hydroxyapatite (CHA) nanoparticles was 25.1±2.2 µg MINO/mg CHA for adsorption over 24 hrs. The alginate microspheres containing minocycline-loaded CHA were biologically active and inhibited the Enterococcus faecalis culture growth for up to seven days of the MINO release. An osteoblastic cell viability assay based on the resazurin reduction was conducted after the cells were exposed to the CHAMINO powder and CHAMINO microspheres. Thus, it was found that the alginate extracts encapsulated the minocycline-loaded CHA microspheres and did not affect the osteoblastic cell viability, while the minocycline-doped CHA powder reduced the cell viability by 90%. Conclusion: This study concluded that the alginate microspheres encapsulating the minocycline-loaded nanocrystalline carbonated hydroxyapatite exhibited combined antibacterial activity against Enterococcus faecalis with cytocompatibility and osteoconduction properties. The significant improvement in the new bone formation after 42 days of implantation suggests that the CHAMINO microsphere has potential in clinical applications of bone regeneration. Dove 2019-06-24 /pmc/articles/PMC6600321/ /pubmed/31417258 http://dx.doi.org/10.2147/IJN.S201631 Text en © 2019 Calasans-Maia et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Calasans-Maia, Mônica Diuana
Barboza Junior, Carlos Alberto Brazil
Soriano-Souza, Carlos Alberto
Alves, Adriana Terezinha Neves Novellino
Uzeda, Marcelo Jose de Pinheiro
Martinez-Zelaya, Victor R
Mavropoulos, Elena
Rocha Leão, Maria Helena
de Santana, Ronaldo Barcellos
Granjeiro, Jose Mauro
Rossi, Alexandre Malta
Microspheres of alginate encapsulated minocycline-loaded nanocrystalline carbonated hydroxyapatite: therapeutic potential and effects on bone regeneration
title Microspheres of alginate encapsulated minocycline-loaded nanocrystalline carbonated hydroxyapatite: therapeutic potential and effects on bone regeneration
title_full Microspheres of alginate encapsulated minocycline-loaded nanocrystalline carbonated hydroxyapatite: therapeutic potential and effects on bone regeneration
title_fullStr Microspheres of alginate encapsulated minocycline-loaded nanocrystalline carbonated hydroxyapatite: therapeutic potential and effects on bone regeneration
title_full_unstemmed Microspheres of alginate encapsulated minocycline-loaded nanocrystalline carbonated hydroxyapatite: therapeutic potential and effects on bone regeneration
title_short Microspheres of alginate encapsulated minocycline-loaded nanocrystalline carbonated hydroxyapatite: therapeutic potential and effects on bone regeneration
title_sort microspheres of alginate encapsulated minocycline-loaded nanocrystalline carbonated hydroxyapatite: therapeutic potential and effects on bone regeneration
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600321/
https://www.ncbi.nlm.nih.gov/pubmed/31417258
http://dx.doi.org/10.2147/IJN.S201631
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