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Deficiency of GD3 Synthase in Mice Resulting in the Attenuation of Bone Loss with Aging

Gangliosides are widely expressed in almost all tissues and cells and are also considered to be essential in the development and maintenance of various organs and tissues. However, little is known about their roles in bone metabolism. In this study, we investigated the effects of genetic deletion of...

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Autores principales: Yo, Shoyoku, Hamamura, Kazunori, Mishima, Yoshitaka, Hamajima, Kosuke, Mori, Hironori, Furukawa, Koichi, Kondo, Hisataka, Tanaka, Kenjiro, Sato, Takuma, Miyazawa, Ken, Goto, Shigemi, Togari, Akifumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600367/
https://www.ncbi.nlm.nih.gov/pubmed/31185614
http://dx.doi.org/10.3390/ijms20112825
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author Yo, Shoyoku
Hamamura, Kazunori
Mishima, Yoshitaka
Hamajima, Kosuke
Mori, Hironori
Furukawa, Koichi
Kondo, Hisataka
Tanaka, Kenjiro
Sato, Takuma
Miyazawa, Ken
Goto, Shigemi
Togari, Akifumi
author_facet Yo, Shoyoku
Hamamura, Kazunori
Mishima, Yoshitaka
Hamajima, Kosuke
Mori, Hironori
Furukawa, Koichi
Kondo, Hisataka
Tanaka, Kenjiro
Sato, Takuma
Miyazawa, Ken
Goto, Shigemi
Togari, Akifumi
author_sort Yo, Shoyoku
collection PubMed
description Gangliosides are widely expressed in almost all tissues and cells and are also considered to be essential in the development and maintenance of various organs and tissues. However, little is known about their roles in bone metabolism. In this study, we investigated the effects of genetic deletion of ganglioside D3 (GD3) synthase, which is responsible for the generation of all b-series gangliosides, on bone metabolism. Although b-series gangliosides were not expressed in osteoblasts, these gangliosides were expressed in pre-osteoclasts. However, the expression of these gangliosides was decreased after induction of osteoclastogenesis by receptor activator of nuclear factor kappa-B ligand (RANKL). Three-dimensional micro-computed tomography (3D-μCT) analysis revealed that femoral cancellous bone mass in GD3 synthase-knockout (GD3S KO) mice was higher than that in wild type (WT) mice at the age of 40 weeks, although there were no differences in that between GD3S KO and WT mice at 15 weeks old. Whereas bone formation parameters (osteoblast numbers/bone surface and osteoblast surface/bone surface) in GD3S KO mice did not differ from WT mice, bone resorption parameters (osteoclast numbers/bone surface and osteoclast surface/bone surface) in GD3S KO mice became significantly lower than those in WT mice at 40 weeks of age. Collectively, this study demonstrates that deletion of GD3 synthase attenuates bone loss that emerges with aging.
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spelling pubmed-66003672019-07-16 Deficiency of GD3 Synthase in Mice Resulting in the Attenuation of Bone Loss with Aging Yo, Shoyoku Hamamura, Kazunori Mishima, Yoshitaka Hamajima, Kosuke Mori, Hironori Furukawa, Koichi Kondo, Hisataka Tanaka, Kenjiro Sato, Takuma Miyazawa, Ken Goto, Shigemi Togari, Akifumi Int J Mol Sci Article Gangliosides are widely expressed in almost all tissues and cells and are also considered to be essential in the development and maintenance of various organs and tissues. However, little is known about their roles in bone metabolism. In this study, we investigated the effects of genetic deletion of ganglioside D3 (GD3) synthase, which is responsible for the generation of all b-series gangliosides, on bone metabolism. Although b-series gangliosides were not expressed in osteoblasts, these gangliosides were expressed in pre-osteoclasts. However, the expression of these gangliosides was decreased after induction of osteoclastogenesis by receptor activator of nuclear factor kappa-B ligand (RANKL). Three-dimensional micro-computed tomography (3D-μCT) analysis revealed that femoral cancellous bone mass in GD3 synthase-knockout (GD3S KO) mice was higher than that in wild type (WT) mice at the age of 40 weeks, although there were no differences in that between GD3S KO and WT mice at 15 weeks old. Whereas bone formation parameters (osteoblast numbers/bone surface and osteoblast surface/bone surface) in GD3S KO mice did not differ from WT mice, bone resorption parameters (osteoclast numbers/bone surface and osteoclast surface/bone surface) in GD3S KO mice became significantly lower than those in WT mice at 40 weeks of age. Collectively, this study demonstrates that deletion of GD3 synthase attenuates bone loss that emerges with aging. MDPI 2019-06-10 /pmc/articles/PMC6600367/ /pubmed/31185614 http://dx.doi.org/10.3390/ijms20112825 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yo, Shoyoku
Hamamura, Kazunori
Mishima, Yoshitaka
Hamajima, Kosuke
Mori, Hironori
Furukawa, Koichi
Kondo, Hisataka
Tanaka, Kenjiro
Sato, Takuma
Miyazawa, Ken
Goto, Shigemi
Togari, Akifumi
Deficiency of GD3 Synthase in Mice Resulting in the Attenuation of Bone Loss with Aging
title Deficiency of GD3 Synthase in Mice Resulting in the Attenuation of Bone Loss with Aging
title_full Deficiency of GD3 Synthase in Mice Resulting in the Attenuation of Bone Loss with Aging
title_fullStr Deficiency of GD3 Synthase in Mice Resulting in the Attenuation of Bone Loss with Aging
title_full_unstemmed Deficiency of GD3 Synthase in Mice Resulting in the Attenuation of Bone Loss with Aging
title_short Deficiency of GD3 Synthase in Mice Resulting in the Attenuation of Bone Loss with Aging
title_sort deficiency of gd3 synthase in mice resulting in the attenuation of bone loss with aging
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600367/
https://www.ncbi.nlm.nih.gov/pubmed/31185614
http://dx.doi.org/10.3390/ijms20112825
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