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A Bifunctional Molecule with Lectin and Protease Inhibitor Activities Isolated from Crataeva tapia Bark Significantly Affects Cocultures of Mesenchymal Stem Cells and Glioblastoma Cells

Currently available drugs for treatment of glioblastoma, the most aggressive brain tumor, remain inefficient, thus a plethora of natural compounds have already been shown to have antimalignant effects. However, these have not been tested for their impact on tumor cells in their microenvironment-simu...

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Autores principales: Bonturi, Camila Ramalho, Silva, Mariana Cristina Cabral, Motaln, Helena, Salu, Bruno Ramos, Ferreira, Rodrigo da Silva, Batista, Fabricio Pereira, Correia, Maria Tereza dos Santos, Paiva, Patrícia Maria Guedes, Turnšek, Tamara Lah, Oliva, Maria Luiza Vilela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600636/
https://www.ncbi.nlm.nih.gov/pubmed/31167364
http://dx.doi.org/10.3390/molecules24112109
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author Bonturi, Camila Ramalho
Silva, Mariana Cristina Cabral
Motaln, Helena
Salu, Bruno Ramos
Ferreira, Rodrigo da Silva
Batista, Fabricio Pereira
Correia, Maria Tereza dos Santos
Paiva, Patrícia Maria Guedes
Turnšek, Tamara Lah
Oliva, Maria Luiza Vilela
author_facet Bonturi, Camila Ramalho
Silva, Mariana Cristina Cabral
Motaln, Helena
Salu, Bruno Ramos
Ferreira, Rodrigo da Silva
Batista, Fabricio Pereira
Correia, Maria Tereza dos Santos
Paiva, Patrícia Maria Guedes
Turnšek, Tamara Lah
Oliva, Maria Luiza Vilela
author_sort Bonturi, Camila Ramalho
collection PubMed
description Currently available drugs for treatment of glioblastoma, the most aggressive brain tumor, remain inefficient, thus a plethora of natural compounds have already been shown to have antimalignant effects. However, these have not been tested for their impact on tumor cells in their microenvironment-simulated cell models, e.g., mesenchymal stem cells in coculture with glioblastoma cell U87 (GB). Mesenchymal stem cells (MSC) chemotactically infiltrate the glioblastoma microenvironment. Our previous studies have shown that bone-marrow derived MSCs impair U87 growth and invasion via paracrine and cell–cell contact-mediated cross-talk. Here, we report on a plant-derived protein, obtained from Crataeva tapia tree Bark Lectin (CrataBL), having protease inhibitory/lectin activities, and demonstrate its effects on glioblastoma cells U87 alone and their cocultures with MSCs. CrataBL inhibited U87 cell invasion and adhesion. Using a simplified model of the stromal microenvironment, i.e., GB/MSC direct cocultures, we demonstrated that CrataBL, when added in increased concentrations, caused cell cycle arrest and decreased cocultured cells’ viability and proliferation, but not invasion. The cocultured cells’ phenotypes were affected by CrataBL via a variety of secreted immunomodulatory cytokines, i.e., G-CSF, GM-CSF, IL-6, IL-8, and VEGF. We hypothesize that CrataBL plays a role by boosting the modulatory effects of MSCs on these glioblastoma cell lines and thus the effects of this and other natural lectins and/or inhibitors would certainly be different in the tumor microenvironment compared to tumor cells alone. We have provided clear evidence that it makes much more sense testing these potential therapeutic adjuvants in cocultures, mimicking heterogeneous tumor–stroma interactions with cancer cells in vivo. As such, CrataBL is suggested as a new candidate to approach adjuvant treatment of this deadly tumor.
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spelling pubmed-66006362019-07-16 A Bifunctional Molecule with Lectin and Protease Inhibitor Activities Isolated from Crataeva tapia Bark Significantly Affects Cocultures of Mesenchymal Stem Cells and Glioblastoma Cells Bonturi, Camila Ramalho Silva, Mariana Cristina Cabral Motaln, Helena Salu, Bruno Ramos Ferreira, Rodrigo da Silva Batista, Fabricio Pereira Correia, Maria Tereza dos Santos Paiva, Patrícia Maria Guedes Turnšek, Tamara Lah Oliva, Maria Luiza Vilela Molecules Article Currently available drugs for treatment of glioblastoma, the most aggressive brain tumor, remain inefficient, thus a plethora of natural compounds have already been shown to have antimalignant effects. However, these have not been tested for their impact on tumor cells in their microenvironment-simulated cell models, e.g., mesenchymal stem cells in coculture with glioblastoma cell U87 (GB). Mesenchymal stem cells (MSC) chemotactically infiltrate the glioblastoma microenvironment. Our previous studies have shown that bone-marrow derived MSCs impair U87 growth and invasion via paracrine and cell–cell contact-mediated cross-talk. Here, we report on a plant-derived protein, obtained from Crataeva tapia tree Bark Lectin (CrataBL), having protease inhibitory/lectin activities, and demonstrate its effects on glioblastoma cells U87 alone and their cocultures with MSCs. CrataBL inhibited U87 cell invasion and adhesion. Using a simplified model of the stromal microenvironment, i.e., GB/MSC direct cocultures, we demonstrated that CrataBL, when added in increased concentrations, caused cell cycle arrest and decreased cocultured cells’ viability and proliferation, but not invasion. The cocultured cells’ phenotypes were affected by CrataBL via a variety of secreted immunomodulatory cytokines, i.e., G-CSF, GM-CSF, IL-6, IL-8, and VEGF. We hypothesize that CrataBL plays a role by boosting the modulatory effects of MSCs on these glioblastoma cell lines and thus the effects of this and other natural lectins and/or inhibitors would certainly be different in the tumor microenvironment compared to tumor cells alone. We have provided clear evidence that it makes much more sense testing these potential therapeutic adjuvants in cocultures, mimicking heterogeneous tumor–stroma interactions with cancer cells in vivo. As such, CrataBL is suggested as a new candidate to approach adjuvant treatment of this deadly tumor. MDPI 2019-06-04 /pmc/articles/PMC6600636/ /pubmed/31167364 http://dx.doi.org/10.3390/molecules24112109 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bonturi, Camila Ramalho
Silva, Mariana Cristina Cabral
Motaln, Helena
Salu, Bruno Ramos
Ferreira, Rodrigo da Silva
Batista, Fabricio Pereira
Correia, Maria Tereza dos Santos
Paiva, Patrícia Maria Guedes
Turnšek, Tamara Lah
Oliva, Maria Luiza Vilela
A Bifunctional Molecule with Lectin and Protease Inhibitor Activities Isolated from Crataeva tapia Bark Significantly Affects Cocultures of Mesenchymal Stem Cells and Glioblastoma Cells
title A Bifunctional Molecule with Lectin and Protease Inhibitor Activities Isolated from Crataeva tapia Bark Significantly Affects Cocultures of Mesenchymal Stem Cells and Glioblastoma Cells
title_full A Bifunctional Molecule with Lectin and Protease Inhibitor Activities Isolated from Crataeva tapia Bark Significantly Affects Cocultures of Mesenchymal Stem Cells and Glioblastoma Cells
title_fullStr A Bifunctional Molecule with Lectin and Protease Inhibitor Activities Isolated from Crataeva tapia Bark Significantly Affects Cocultures of Mesenchymal Stem Cells and Glioblastoma Cells
title_full_unstemmed A Bifunctional Molecule with Lectin and Protease Inhibitor Activities Isolated from Crataeva tapia Bark Significantly Affects Cocultures of Mesenchymal Stem Cells and Glioblastoma Cells
title_short A Bifunctional Molecule with Lectin and Protease Inhibitor Activities Isolated from Crataeva tapia Bark Significantly Affects Cocultures of Mesenchymal Stem Cells and Glioblastoma Cells
title_sort bifunctional molecule with lectin and protease inhibitor activities isolated from crataeva tapia bark significantly affects cocultures of mesenchymal stem cells and glioblastoma cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600636/
https://www.ncbi.nlm.nih.gov/pubmed/31167364
http://dx.doi.org/10.3390/molecules24112109
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