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Galectin-9 Induces Mitochondria-Mediated Apoptosis of Esophageal Cancer In Vitro and In Vivo in a Xenograft Mouse Model

Galectin-9 (Gal-9) enhances tumor immunity mediated by T cells, macrophages, and dendritic cells. Its expression level in various cancers correlates with prognosis. Furthermore, Gal-9 directly induces apoptosis in various cancers; however, its mechanism of action and bioactivity has not been clarifi...

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Autores principales: Chiyo, Taiga, Fujita, Koji, Iwama, Hisakazu, Fujihara, Shintaro, Tadokoro, Tomoko, Ohura, Kyoko, Matsui, Takanori, Goda, Yasuhiro, Kobayashi, Nobuya, Nishiyama, Noriko, Yachida, Tatsuo, Morishita, Asahiro, Kobara, Hideki, Mori, Hirohito, Niki, Toshiro, Hirashima, Mitsuomi, Himoto, Takashi, Masaki, Tsutomu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600680/
https://www.ncbi.nlm.nih.gov/pubmed/31146370
http://dx.doi.org/10.3390/ijms20112634
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author Chiyo, Taiga
Fujita, Koji
Iwama, Hisakazu
Fujihara, Shintaro
Tadokoro, Tomoko
Ohura, Kyoko
Matsui, Takanori
Goda, Yasuhiro
Kobayashi, Nobuya
Nishiyama, Noriko
Yachida, Tatsuo
Morishita, Asahiro
Kobara, Hideki
Mori, Hirohito
Niki, Toshiro
Hirashima, Mitsuomi
Himoto, Takashi
Masaki, Tsutomu
author_facet Chiyo, Taiga
Fujita, Koji
Iwama, Hisakazu
Fujihara, Shintaro
Tadokoro, Tomoko
Ohura, Kyoko
Matsui, Takanori
Goda, Yasuhiro
Kobayashi, Nobuya
Nishiyama, Noriko
Yachida, Tatsuo
Morishita, Asahiro
Kobara, Hideki
Mori, Hirohito
Niki, Toshiro
Hirashima, Mitsuomi
Himoto, Takashi
Masaki, Tsutomu
author_sort Chiyo, Taiga
collection PubMed
description Galectin-9 (Gal-9) enhances tumor immunity mediated by T cells, macrophages, and dendritic cells. Its expression level in various cancers correlates with prognosis. Furthermore, Gal-9 directly induces apoptosis in various cancers; however, its mechanism of action and bioactivity has not been clarified. We evaluated Gal-9 antitumor effect against esophageal squamous cell carcinoma (ESCC) to analyze the dynamics of apoptosis-related molecules, elucidate its mechanism of action, and identify relevant changes in miRNA expressions. KYSE-150 and KYSE-180 cells were treated with Gal-9 and their proliferation was evaluated. Gal-9 inhibited cell proliferation in a concentration-dependent manner. The xenograft mouse model established with KYSE-150 cells was administered with Gal-9 and significant suppression in the tumor growth observed. Gal-9 treatment of KYSE-150 cells increased the number of Annexin V-positive cells, activation of caspase-3, and collapse of mitochondrial potential, indicating apoptosis induction. c-Jun NH(2)-terminal kinase (JNK) and p38 mitogen-activated protein kinase (p38) phosphorylation were activated and could be involved in apoptosis. Therefore, Gal-9 induces mitochondria-mediated apoptosis of ESCC and inhibits cell proliferation in vitro and in vivo with JNK and p38 activation.
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spelling pubmed-66006802019-07-16 Galectin-9 Induces Mitochondria-Mediated Apoptosis of Esophageal Cancer In Vitro and In Vivo in a Xenograft Mouse Model Chiyo, Taiga Fujita, Koji Iwama, Hisakazu Fujihara, Shintaro Tadokoro, Tomoko Ohura, Kyoko Matsui, Takanori Goda, Yasuhiro Kobayashi, Nobuya Nishiyama, Noriko Yachida, Tatsuo Morishita, Asahiro Kobara, Hideki Mori, Hirohito Niki, Toshiro Hirashima, Mitsuomi Himoto, Takashi Masaki, Tsutomu Int J Mol Sci Article Galectin-9 (Gal-9) enhances tumor immunity mediated by T cells, macrophages, and dendritic cells. Its expression level in various cancers correlates with prognosis. Furthermore, Gal-9 directly induces apoptosis in various cancers; however, its mechanism of action and bioactivity has not been clarified. We evaluated Gal-9 antitumor effect against esophageal squamous cell carcinoma (ESCC) to analyze the dynamics of apoptosis-related molecules, elucidate its mechanism of action, and identify relevant changes in miRNA expressions. KYSE-150 and KYSE-180 cells were treated with Gal-9 and their proliferation was evaluated. Gal-9 inhibited cell proliferation in a concentration-dependent manner. The xenograft mouse model established with KYSE-150 cells was administered with Gal-9 and significant suppression in the tumor growth observed. Gal-9 treatment of KYSE-150 cells increased the number of Annexin V-positive cells, activation of caspase-3, and collapse of mitochondrial potential, indicating apoptosis induction. c-Jun NH(2)-terminal kinase (JNK) and p38 mitogen-activated protein kinase (p38) phosphorylation were activated and could be involved in apoptosis. Therefore, Gal-9 induces mitochondria-mediated apoptosis of ESCC and inhibits cell proliferation in vitro and in vivo with JNK and p38 activation. MDPI 2019-05-29 /pmc/articles/PMC6600680/ /pubmed/31146370 http://dx.doi.org/10.3390/ijms20112634 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chiyo, Taiga
Fujita, Koji
Iwama, Hisakazu
Fujihara, Shintaro
Tadokoro, Tomoko
Ohura, Kyoko
Matsui, Takanori
Goda, Yasuhiro
Kobayashi, Nobuya
Nishiyama, Noriko
Yachida, Tatsuo
Morishita, Asahiro
Kobara, Hideki
Mori, Hirohito
Niki, Toshiro
Hirashima, Mitsuomi
Himoto, Takashi
Masaki, Tsutomu
Galectin-9 Induces Mitochondria-Mediated Apoptosis of Esophageal Cancer In Vitro and In Vivo in a Xenograft Mouse Model
title Galectin-9 Induces Mitochondria-Mediated Apoptosis of Esophageal Cancer In Vitro and In Vivo in a Xenograft Mouse Model
title_full Galectin-9 Induces Mitochondria-Mediated Apoptosis of Esophageal Cancer In Vitro and In Vivo in a Xenograft Mouse Model
title_fullStr Galectin-9 Induces Mitochondria-Mediated Apoptosis of Esophageal Cancer In Vitro and In Vivo in a Xenograft Mouse Model
title_full_unstemmed Galectin-9 Induces Mitochondria-Mediated Apoptosis of Esophageal Cancer In Vitro and In Vivo in a Xenograft Mouse Model
title_short Galectin-9 Induces Mitochondria-Mediated Apoptosis of Esophageal Cancer In Vitro and In Vivo in a Xenograft Mouse Model
title_sort galectin-9 induces mitochondria-mediated apoptosis of esophageal cancer in vitro and in vivo in a xenograft mouse model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600680/
https://www.ncbi.nlm.nih.gov/pubmed/31146370
http://dx.doi.org/10.3390/ijms20112634
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