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Polyethylene Terephthalate Textiles Enhance the Structural Maturation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have the potential to serve as a model for human cardiomyocytes. However, hiPSC-CMs are still considered immature. CMs differentiated from hiPSCs more resemble fetal than adult cardiomyocytes. Putative factors enhancing maturatio...

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Autores principales: Pekkanen-Mattila, Mari, Häkli, Martta, Pölönen, Risto-Pekka, Mansikkala, Tuomas, Junnila, Anni, Talvitie, Elina, Koivisto, Janne T, Kellomäki, Minna, Aalto-Setälä, Katriina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600740/
https://www.ncbi.nlm.nih.gov/pubmed/31163704
http://dx.doi.org/10.3390/ma12111805
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author Pekkanen-Mattila, Mari
Häkli, Martta
Pölönen, Risto-Pekka
Mansikkala, Tuomas
Junnila, Anni
Talvitie, Elina
Koivisto, Janne T
Kellomäki, Minna
Aalto-Setälä, Katriina
author_facet Pekkanen-Mattila, Mari
Häkli, Martta
Pölönen, Risto-Pekka
Mansikkala, Tuomas
Junnila, Anni
Talvitie, Elina
Koivisto, Janne T
Kellomäki, Minna
Aalto-Setälä, Katriina
author_sort Pekkanen-Mattila, Mari
collection PubMed
description Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have the potential to serve as a model for human cardiomyocytes. However, hiPSC-CMs are still considered immature. CMs differentiated from hiPSCs more resemble fetal than adult cardiomyocytes. Putative factors enhancing maturation include in vitro culture duration, culture surface topography, and mechanical, chemical, and electrical stimulation. Stem cell-derived cardiomyocytes are traditionally cultured on glass surfaces coated with extracellular matrix derivatives such as gelatin. hiPSC-CMs are flat and round and their sarcomeres are randomly distributed and unorganized. Morphology can be enhanced by culturing cells on surfaces providing topographical cues to the cells. In this study, a textile based-culturing method used to enhance the maturation status of hiPSC-CMs is presented. Gelatin-coated polyethylene terephthalate (PET)-based textiles were used as the culturing surface for hiPSC-CMs and the effects of the textiles on the maturation status of the hiPSC-CMs were assessed. The hiPSC-CMs were characterized by analyzing their morphology, sarcomere organization, expression of cardiac specific genes, and calcium handling. We show that the topographical cues improve the structure of the hiPSC-CMs in vitro. Human iPSC-CMs grown on PET textiles demonstrated improved structural properties such as rod-shape structure and increased sarcomere orientation.
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spelling pubmed-66007402019-07-16 Polyethylene Terephthalate Textiles Enhance the Structural Maturation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes Pekkanen-Mattila, Mari Häkli, Martta Pölönen, Risto-Pekka Mansikkala, Tuomas Junnila, Anni Talvitie, Elina Koivisto, Janne T Kellomäki, Minna Aalto-Setälä, Katriina Materials (Basel) Article Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have the potential to serve as a model for human cardiomyocytes. However, hiPSC-CMs are still considered immature. CMs differentiated from hiPSCs more resemble fetal than adult cardiomyocytes. Putative factors enhancing maturation include in vitro culture duration, culture surface topography, and mechanical, chemical, and electrical stimulation. Stem cell-derived cardiomyocytes are traditionally cultured on glass surfaces coated with extracellular matrix derivatives such as gelatin. hiPSC-CMs are flat and round and their sarcomeres are randomly distributed and unorganized. Morphology can be enhanced by culturing cells on surfaces providing topographical cues to the cells. In this study, a textile based-culturing method used to enhance the maturation status of hiPSC-CMs is presented. Gelatin-coated polyethylene terephthalate (PET)-based textiles were used as the culturing surface for hiPSC-CMs and the effects of the textiles on the maturation status of the hiPSC-CMs were assessed. The hiPSC-CMs were characterized by analyzing their morphology, sarcomere organization, expression of cardiac specific genes, and calcium handling. We show that the topographical cues improve the structure of the hiPSC-CMs in vitro. Human iPSC-CMs grown on PET textiles demonstrated improved structural properties such as rod-shape structure and increased sarcomere orientation. MDPI 2019-06-03 /pmc/articles/PMC6600740/ /pubmed/31163704 http://dx.doi.org/10.3390/ma12111805 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pekkanen-Mattila, Mari
Häkli, Martta
Pölönen, Risto-Pekka
Mansikkala, Tuomas
Junnila, Anni
Talvitie, Elina
Koivisto, Janne T
Kellomäki, Minna
Aalto-Setälä, Katriina
Polyethylene Terephthalate Textiles Enhance the Structural Maturation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
title Polyethylene Terephthalate Textiles Enhance the Structural Maturation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
title_full Polyethylene Terephthalate Textiles Enhance the Structural Maturation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
title_fullStr Polyethylene Terephthalate Textiles Enhance the Structural Maturation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
title_full_unstemmed Polyethylene Terephthalate Textiles Enhance the Structural Maturation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
title_short Polyethylene Terephthalate Textiles Enhance the Structural Maturation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
title_sort polyethylene terephthalate textiles enhance the structural maturation of human induced pluripotent stem cell-derived cardiomyocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600740/
https://www.ncbi.nlm.nih.gov/pubmed/31163704
http://dx.doi.org/10.3390/ma12111805
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