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Isolation, characterization, and effect of phosphate-zinc-solubilizing bacterial strains on chickpea (Cicer arietinum L.) growth

OBJECTIVE: Phosphate (P) and zinc (Zn) are essential plant nutrients required for nodulation, nitrogen-fixation, plant growth and yield. Mostly applied P and Zn nutrients in the soil are converted into unavailable form. A small number of soil microbes have the ability to transform unsolvable forms o...

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Autores principales: Zaheer, Ahmad, Malik, Arif, Sher, Ahmad, Mansoor Qaisrani, Muther, Mehmood, Asim, Ullah Khan, Sami, Ashraf, Muhammad, Mirza, Zeenat, Karim, Sajjad, Rasool, Mahmood
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600776/
https://www.ncbi.nlm.nih.gov/pubmed/31303841
http://dx.doi.org/10.1016/j.sjbs.2019.04.004
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author Zaheer, Ahmad
Malik, Arif
Sher, Ahmad
Mansoor Qaisrani, Muther
Mehmood, Asim
Ullah Khan, Sami
Ashraf, Muhammad
Mirza, Zeenat
Karim, Sajjad
Rasool, Mahmood
author_facet Zaheer, Ahmad
Malik, Arif
Sher, Ahmad
Mansoor Qaisrani, Muther
Mehmood, Asim
Ullah Khan, Sami
Ashraf, Muhammad
Mirza, Zeenat
Karim, Sajjad
Rasool, Mahmood
author_sort Zaheer, Ahmad
collection PubMed
description OBJECTIVE: Phosphate (P) and zinc (Zn) are essential plant nutrients required for nodulation, nitrogen-fixation, plant growth and yield. Mostly applied P and Zn nutrients in the soil are converted into unavailable form. A small number of soil microbes have the ability to transform unsolvable forms of P and Zn to an available form. P-Zn-solubilizing rhizobacteria are potential alternates for P and Zn supplement. In the present study, the effect of two P-Zn-solubilizing bacterial strains (Bacillus sp. strain AZ17 and Pseudomonas sp. strain AZ5) was evaluated on the growth of chickpea plant. METHODOLOGY: Both strains were purified from the rhizospheric soil of chickpea plant grown-up in sandy soil and rain-fed area (Thal desert). In vitro, both strains solubilize P and Zn as well both strain produce IAA and organic acids. In the field experiments, conducted in the rain-fed area, the positive influence of inoculation with both bacterial isolates AZ5 and AZ17 on chickpea growth was observed. RESULTS: The application of inoculum (strains AZ5 and AZ17) resulted in up to 17.47% and 17.34% increase in grain yield of both types of chickpea grown in fertilized and non-fertilized soil, respectively over non-inoculated control. Strain AZ5 was the most effective inoculum, increasing up to 17.47%, 16.04%, 26.32%, 22.53%, 26.12% and 22.59% in grain yield, straw weight, nodules number, dry weight of nodules, Zn uptake and P uptake respectively, over control. CONCLUSION: These results indicated that Pseudomonas sp. strain AZ5 and Bacillus sp. strain AZ17 can serve as effective microbial inocula for chickpea, particularly in the rain-fed area.
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spelling pubmed-66007762019-07-12 Isolation, characterization, and effect of phosphate-zinc-solubilizing bacterial strains on chickpea (Cicer arietinum L.) growth Zaheer, Ahmad Malik, Arif Sher, Ahmad Mansoor Qaisrani, Muther Mehmood, Asim Ullah Khan, Sami Ashraf, Muhammad Mirza, Zeenat Karim, Sajjad Rasool, Mahmood Saudi J Biol Sci Article OBJECTIVE: Phosphate (P) and zinc (Zn) are essential plant nutrients required for nodulation, nitrogen-fixation, plant growth and yield. Mostly applied P and Zn nutrients in the soil are converted into unavailable form. A small number of soil microbes have the ability to transform unsolvable forms of P and Zn to an available form. P-Zn-solubilizing rhizobacteria are potential alternates for P and Zn supplement. In the present study, the effect of two P-Zn-solubilizing bacterial strains (Bacillus sp. strain AZ17 and Pseudomonas sp. strain AZ5) was evaluated on the growth of chickpea plant. METHODOLOGY: Both strains were purified from the rhizospheric soil of chickpea plant grown-up in sandy soil and rain-fed area (Thal desert). In vitro, both strains solubilize P and Zn as well both strain produce IAA and organic acids. In the field experiments, conducted in the rain-fed area, the positive influence of inoculation with both bacterial isolates AZ5 and AZ17 on chickpea growth was observed. RESULTS: The application of inoculum (strains AZ5 and AZ17) resulted in up to 17.47% and 17.34% increase in grain yield of both types of chickpea grown in fertilized and non-fertilized soil, respectively over non-inoculated control. Strain AZ5 was the most effective inoculum, increasing up to 17.47%, 16.04%, 26.32%, 22.53%, 26.12% and 22.59% in grain yield, straw weight, nodules number, dry weight of nodules, Zn uptake and P uptake respectively, over control. CONCLUSION: These results indicated that Pseudomonas sp. strain AZ5 and Bacillus sp. strain AZ17 can serve as effective microbial inocula for chickpea, particularly in the rain-fed area. Elsevier 2019-07 2019-04-08 /pmc/articles/PMC6600776/ /pubmed/31303841 http://dx.doi.org/10.1016/j.sjbs.2019.04.004 Text en © 2019 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Zaheer, Ahmad
Malik, Arif
Sher, Ahmad
Mansoor Qaisrani, Muther
Mehmood, Asim
Ullah Khan, Sami
Ashraf, Muhammad
Mirza, Zeenat
Karim, Sajjad
Rasool, Mahmood
Isolation, characterization, and effect of phosphate-zinc-solubilizing bacterial strains on chickpea (Cicer arietinum L.) growth
title Isolation, characterization, and effect of phosphate-zinc-solubilizing bacterial strains on chickpea (Cicer arietinum L.) growth
title_full Isolation, characterization, and effect of phosphate-zinc-solubilizing bacterial strains on chickpea (Cicer arietinum L.) growth
title_fullStr Isolation, characterization, and effect of phosphate-zinc-solubilizing bacterial strains on chickpea (Cicer arietinum L.) growth
title_full_unstemmed Isolation, characterization, and effect of phosphate-zinc-solubilizing bacterial strains on chickpea (Cicer arietinum L.) growth
title_short Isolation, characterization, and effect of phosphate-zinc-solubilizing bacterial strains on chickpea (Cicer arietinum L.) growth
title_sort isolation, characterization, and effect of phosphate-zinc-solubilizing bacterial strains on chickpea (cicer arietinum l.) growth
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600776/
https://www.ncbi.nlm.nih.gov/pubmed/31303841
http://dx.doi.org/10.1016/j.sjbs.2019.04.004
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