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Regenerative Features of Adipose Tissue for Osteoarthritis Treatment in a Rabbit Model: Enzymatic Digestion Versus Mechanical Disruption

Evaluating cell migration after cell-based treatment is important for several disorders, including osteoarthritis (OA), as it might influence the clinical outcome. This research explores migrating expanded-adipose stromal cells (ASCs) and adipose niches after enzymatic and mechanical processes. Bila...

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Autores principales: Desando, Giovanna, Bartolotti, Isabella, Martini, Lucia, Giavaresi, Gianluca, Nicoli Aldini, Nicolò, Fini, Milena, Roffi, Alice, Perdisa, Francesco, Filardo, Giuseppe, Kon, Elizaveta, Grigolo, Brunella
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6601012/
https://www.ncbi.nlm.nih.gov/pubmed/31146351
http://dx.doi.org/10.3390/ijms20112636
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author Desando, Giovanna
Bartolotti, Isabella
Martini, Lucia
Giavaresi, Gianluca
Nicoli Aldini, Nicolò
Fini, Milena
Roffi, Alice
Perdisa, Francesco
Filardo, Giuseppe
Kon, Elizaveta
Grigolo, Brunella
author_facet Desando, Giovanna
Bartolotti, Isabella
Martini, Lucia
Giavaresi, Gianluca
Nicoli Aldini, Nicolò
Fini, Milena
Roffi, Alice
Perdisa, Francesco
Filardo, Giuseppe
Kon, Elizaveta
Grigolo, Brunella
author_sort Desando, Giovanna
collection PubMed
description Evaluating cell migration after cell-based treatment is important for several disorders, including osteoarthritis (OA), as it might influence the clinical outcome. This research explores migrating expanded-adipose stromal cells (ASCs) and adipose niches after enzymatic and mechanical processes. Bilateral anterior cruciate ligament transection induced a mild grade of OA at eight weeks in adult male New Zealand rabbits. ASCs, enzymatic stromal vascular fraction (SVF), and micro fragmented adipose tissue (MFAT) were intra-articularly injected in the knee joint. Assessments of cell viability and expression of specific markers, including CD-163 wound-healing macrophages, were done. Cell migration was explored through labelling with PKH26 dye at 7 and 30 days alongside co-localization analyses for CD-146. All cells showed good viability and high percentages of CD-90 and CD-146. CD-163 was significantly higher in MFAT compared to SVF. Distinct migratory potential and time-dependent effects were observed among cell-based treatments. At day 7, both ASCs and SVF migrated towards synovium, whereas for MFAT versus cartilage, a different migration pattern was noticed at day 30. The long-term distinct cell migration of ASCs, SVF, and MFAT open interesting clinical insights on their potential use for OA treatment. Moreover, the highest expression of CD-163 in MFAT, rather than SVF, might have an important role in directly mediating cartilage tissue repair responses.
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spelling pubmed-66010122019-07-18 Regenerative Features of Adipose Tissue for Osteoarthritis Treatment in a Rabbit Model: Enzymatic Digestion Versus Mechanical Disruption Desando, Giovanna Bartolotti, Isabella Martini, Lucia Giavaresi, Gianluca Nicoli Aldini, Nicolò Fini, Milena Roffi, Alice Perdisa, Francesco Filardo, Giuseppe Kon, Elizaveta Grigolo, Brunella Int J Mol Sci Article Evaluating cell migration after cell-based treatment is important for several disorders, including osteoarthritis (OA), as it might influence the clinical outcome. This research explores migrating expanded-adipose stromal cells (ASCs) and adipose niches after enzymatic and mechanical processes. Bilateral anterior cruciate ligament transection induced a mild grade of OA at eight weeks in adult male New Zealand rabbits. ASCs, enzymatic stromal vascular fraction (SVF), and micro fragmented adipose tissue (MFAT) were intra-articularly injected in the knee joint. Assessments of cell viability and expression of specific markers, including CD-163 wound-healing macrophages, were done. Cell migration was explored through labelling with PKH26 dye at 7 and 30 days alongside co-localization analyses for CD-146. All cells showed good viability and high percentages of CD-90 and CD-146. CD-163 was significantly higher in MFAT compared to SVF. Distinct migratory potential and time-dependent effects were observed among cell-based treatments. At day 7, both ASCs and SVF migrated towards synovium, whereas for MFAT versus cartilage, a different migration pattern was noticed at day 30. The long-term distinct cell migration of ASCs, SVF, and MFAT open interesting clinical insights on their potential use for OA treatment. Moreover, the highest expression of CD-163 in MFAT, rather than SVF, might have an important role in directly mediating cartilage tissue repair responses. MDPI 2019-05-29 /pmc/articles/PMC6601012/ /pubmed/31146351 http://dx.doi.org/10.3390/ijms20112636 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Desando, Giovanna
Bartolotti, Isabella
Martini, Lucia
Giavaresi, Gianluca
Nicoli Aldini, Nicolò
Fini, Milena
Roffi, Alice
Perdisa, Francesco
Filardo, Giuseppe
Kon, Elizaveta
Grigolo, Brunella
Regenerative Features of Adipose Tissue for Osteoarthritis Treatment in a Rabbit Model: Enzymatic Digestion Versus Mechanical Disruption
title Regenerative Features of Adipose Tissue for Osteoarthritis Treatment in a Rabbit Model: Enzymatic Digestion Versus Mechanical Disruption
title_full Regenerative Features of Adipose Tissue for Osteoarthritis Treatment in a Rabbit Model: Enzymatic Digestion Versus Mechanical Disruption
title_fullStr Regenerative Features of Adipose Tissue for Osteoarthritis Treatment in a Rabbit Model: Enzymatic Digestion Versus Mechanical Disruption
title_full_unstemmed Regenerative Features of Adipose Tissue for Osteoarthritis Treatment in a Rabbit Model: Enzymatic Digestion Versus Mechanical Disruption
title_short Regenerative Features of Adipose Tissue for Osteoarthritis Treatment in a Rabbit Model: Enzymatic Digestion Versus Mechanical Disruption
title_sort regenerative features of adipose tissue for osteoarthritis treatment in a rabbit model: enzymatic digestion versus mechanical disruption
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6601012/
https://www.ncbi.nlm.nih.gov/pubmed/31146351
http://dx.doi.org/10.3390/ijms20112636
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