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Identification of duck liver-expressed antimicrobial peptide 2 and characterization of its bactericidal activity
OBJECTIVE: This study was conducted to identify duck liver-expressed antimicrobial peptide 2 (LEAP-2) and demonstrate its antimicrobial activity against various pathogens. METHODS: Tissue samples were collected from 6 to 8-week-old Pekin ducks (Anas platyrhynchos domesticus), total RNA was extracted...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST)
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6601062/ https://www.ncbi.nlm.nih.gov/pubmed/30381731 http://dx.doi.org/10.5713/ajas.18.0571 |
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author | Hong, Yeojin Truong, Anh Duc Lee, Janggeun Lee, Kyungbaek Kim, Geun-Bae Heo, Kang-Nyeong Lillehoj, Hyun S. Hong, Yeong Ho |
author_facet | Hong, Yeojin Truong, Anh Duc Lee, Janggeun Lee, Kyungbaek Kim, Geun-Bae Heo, Kang-Nyeong Lillehoj, Hyun S. Hong, Yeong Ho |
author_sort | Hong, Yeojin |
collection | PubMed |
description | OBJECTIVE: This study was conducted to identify duck liver-expressed antimicrobial peptide 2 (LEAP-2) and demonstrate its antimicrobial activity against various pathogens. METHODS: Tissue samples were collected from 6 to 8-week-old Pekin ducks (Anas platyrhynchos domesticus), total RNA was extracted, and cDNA was synthesized. To confirm the duck LEAP-2 transcript expression levels, quantitative real-time polymerase chain reaction was conducted. Two kinds of peptides (a linear peptide and a disulfide-type peptide) were synthesized to compare the antimicrobial activity. Then, antimicrobial activity assay and fluorescence microscopic analysis were conducted to demonstrate duck LEAP-2 bactericidal activity. RESULTS: The duck LEAP-2 peptide sequence showed high identity with those of other avian species (>85%), as well as more than 55% of identity with mammalian sequences. LEAP-2 mRNA was highly expressed in the liver with duodenum next, and then followed by lung, spleen, bursa and jejunum and was the lowest in the muscle. Both of LEAP-2 peptides efficiently killed bacteria, although the disulfide-type LEAP-2 showed more powerful bactericidal activity. Also, gram-positive bacteria was more susceptible to duck LEAP-2 than gram-negative bacteria. Using microscopy, we confirmed that LEAP-2 peptides could kill bacteria by disrupting the bacterial cell envelope. CONCLUSION: Duck LEAP-2 showed its antimicrobial activity against both gram-positive and gram-negative bacteria. Disulfide bonds were important for the powerful killing effect by disrupting the bacterial cell envelope. Therefore, duck LEAP-2 can be used for effective antibiotics alternatives. |
format | Online Article Text |
id | pubmed-6601062 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST) |
record_format | MEDLINE/PubMed |
spelling | pubmed-66010622019-07-10 Identification of duck liver-expressed antimicrobial peptide 2 and characterization of its bactericidal activity Hong, Yeojin Truong, Anh Duc Lee, Janggeun Lee, Kyungbaek Kim, Geun-Bae Heo, Kang-Nyeong Lillehoj, Hyun S. Hong, Yeong Ho Asian-Australas J Anim Sci Article OBJECTIVE: This study was conducted to identify duck liver-expressed antimicrobial peptide 2 (LEAP-2) and demonstrate its antimicrobial activity against various pathogens. METHODS: Tissue samples were collected from 6 to 8-week-old Pekin ducks (Anas platyrhynchos domesticus), total RNA was extracted, and cDNA was synthesized. To confirm the duck LEAP-2 transcript expression levels, quantitative real-time polymerase chain reaction was conducted. Two kinds of peptides (a linear peptide and a disulfide-type peptide) were synthesized to compare the antimicrobial activity. Then, antimicrobial activity assay and fluorescence microscopic analysis were conducted to demonstrate duck LEAP-2 bactericidal activity. RESULTS: The duck LEAP-2 peptide sequence showed high identity with those of other avian species (>85%), as well as more than 55% of identity with mammalian sequences. LEAP-2 mRNA was highly expressed in the liver with duodenum next, and then followed by lung, spleen, bursa and jejunum and was the lowest in the muscle. Both of LEAP-2 peptides efficiently killed bacteria, although the disulfide-type LEAP-2 showed more powerful bactericidal activity. Also, gram-positive bacteria was more susceptible to duck LEAP-2 than gram-negative bacteria. Using microscopy, we confirmed that LEAP-2 peptides could kill bacteria by disrupting the bacterial cell envelope. CONCLUSION: Duck LEAP-2 showed its antimicrobial activity against both gram-positive and gram-negative bacteria. Disulfide bonds were important for the powerful killing effect by disrupting the bacterial cell envelope. Therefore, duck LEAP-2 can be used for effective antibiotics alternatives. Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST) 2019-07 2018-10-29 /pmc/articles/PMC6601062/ /pubmed/30381731 http://dx.doi.org/10.5713/ajas.18.0571 Text en Copyright © 2019 by Asian-Australasian Journal of Animal Sciences This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Hong, Yeojin Truong, Anh Duc Lee, Janggeun Lee, Kyungbaek Kim, Geun-Bae Heo, Kang-Nyeong Lillehoj, Hyun S. Hong, Yeong Ho Identification of duck liver-expressed antimicrobial peptide 2 and characterization of its bactericidal activity |
title | Identification of duck liver-expressed antimicrobial peptide 2 and characterization of its bactericidal activity |
title_full | Identification of duck liver-expressed antimicrobial peptide 2 and characterization of its bactericidal activity |
title_fullStr | Identification of duck liver-expressed antimicrobial peptide 2 and characterization of its bactericidal activity |
title_full_unstemmed | Identification of duck liver-expressed antimicrobial peptide 2 and characterization of its bactericidal activity |
title_short | Identification of duck liver-expressed antimicrobial peptide 2 and characterization of its bactericidal activity |
title_sort | identification of duck liver-expressed antimicrobial peptide 2 and characterization of its bactericidal activity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6601062/ https://www.ncbi.nlm.nih.gov/pubmed/30381731 http://dx.doi.org/10.5713/ajas.18.0571 |
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