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Curcumin suppresses intestinal microvascular endothelial cells invasion and angiogenesis induced by activated platelets

The present study investigated the effects and mechanism by which curcumin suppresses intestinal microvascular endothelial cells (INMECs) invasion and angiogenesis induced by activated platelets. INMECs were obtained from healthy rats, and divided into five groups: Control, platelets, platelets +2.5...

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Autores principales: Xu, Su, Xu, Zhao-Xiu, Yan, Shuai, Le, Jin, Chen, Hao, Ming, Lan, Xu, Shu-Guang, Lin, Tao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6601414/
https://www.ncbi.nlm.nih.gov/pubmed/31316605
http://dx.doi.org/10.3892/etm.2019.7662
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author Xu, Su
Xu, Zhao-Xiu
Yan, Shuai
Le, Jin
Chen, Hao
Ming, Lan
Xu, Shu-Guang
Lin, Tao
author_facet Xu, Su
Xu, Zhao-Xiu
Yan, Shuai
Le, Jin
Chen, Hao
Ming, Lan
Xu, Shu-Guang
Lin, Tao
author_sort Xu, Su
collection PubMed
description The present study investigated the effects and mechanism by which curcumin suppresses intestinal microvascular endothelial cells (INMECs) invasion and angiogenesis induced by activated platelets. INMECs were obtained from healthy rats, and divided into five groups: Control, platelets, platelets +2.5 µM curcumin, platelets +5.0 µM curcumin and platelets +10.0 µM curcumin. Curcumin toxicity was determined and vascular endothelial growth factor (VEGF) concentrations of the five groups were measured using ELISA. The branch point numbers were measured using a capillary tube formation experiment, invasion cell numbers were evaluated with the Transwell assay, relative protein expression levels were measured with western blot assay and immunofluorescence staining of the nucleus. The 2.5, 5 and 10 µM curcumin concentrations were found to be suitable for INMECs. Curcumin significantly downregulated VEGF concentration, suppressed vascular lumen formation and inhibited invasion cell numbers in a dose-dependent manner. The α-smooth muscle actin, collagen I, E-cadherin, phosphorylated (p-) phosphoinositide 3-kinase (PI3K), p-protein kinase B (AKT), p-mammalian target of rapamycin (m-TOR) and hypoxia inducible factor subunit alpha (HIF-1α) protein expression levels of the curcumin-treated groups were significantly downregulated in a dose-dependent manner compared with the platelet group. HIF-1α protein expression levels in the nucleus of the curcumin-treated groups were significantly suppressed in a dose-dependent manner compared with the platelet group. In conclusion, curcumin suppressed INMEC invasion and angiogenesis induced by activated platelets via inhibiting the activation of the PI3K/AKT/mTOR pathway.
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spelling pubmed-66014142019-07-17 Curcumin suppresses intestinal microvascular endothelial cells invasion and angiogenesis induced by activated platelets Xu, Su Xu, Zhao-Xiu Yan, Shuai Le, Jin Chen, Hao Ming, Lan Xu, Shu-Guang Lin, Tao Exp Ther Med Articles The present study investigated the effects and mechanism by which curcumin suppresses intestinal microvascular endothelial cells (INMECs) invasion and angiogenesis induced by activated platelets. INMECs were obtained from healthy rats, and divided into five groups: Control, platelets, platelets +2.5 µM curcumin, platelets +5.0 µM curcumin and platelets +10.0 µM curcumin. Curcumin toxicity was determined and vascular endothelial growth factor (VEGF) concentrations of the five groups were measured using ELISA. The branch point numbers were measured using a capillary tube formation experiment, invasion cell numbers were evaluated with the Transwell assay, relative protein expression levels were measured with western blot assay and immunofluorescence staining of the nucleus. The 2.5, 5 and 10 µM curcumin concentrations were found to be suitable for INMECs. Curcumin significantly downregulated VEGF concentration, suppressed vascular lumen formation and inhibited invasion cell numbers in a dose-dependent manner. The α-smooth muscle actin, collagen I, E-cadherin, phosphorylated (p-) phosphoinositide 3-kinase (PI3K), p-protein kinase B (AKT), p-mammalian target of rapamycin (m-TOR) and hypoxia inducible factor subunit alpha (HIF-1α) protein expression levels of the curcumin-treated groups were significantly downregulated in a dose-dependent manner compared with the platelet group. HIF-1α protein expression levels in the nucleus of the curcumin-treated groups were significantly suppressed in a dose-dependent manner compared with the platelet group. In conclusion, curcumin suppressed INMEC invasion and angiogenesis induced by activated platelets via inhibiting the activation of the PI3K/AKT/mTOR pathway. D.A. Spandidos 2019-08 2019-06-11 /pmc/articles/PMC6601414/ /pubmed/31316605 http://dx.doi.org/10.3892/etm.2019.7662 Text en Copyright: © Xu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Xu, Su
Xu, Zhao-Xiu
Yan, Shuai
Le, Jin
Chen, Hao
Ming, Lan
Xu, Shu-Guang
Lin, Tao
Curcumin suppresses intestinal microvascular endothelial cells invasion and angiogenesis induced by activated platelets
title Curcumin suppresses intestinal microvascular endothelial cells invasion and angiogenesis induced by activated platelets
title_full Curcumin suppresses intestinal microvascular endothelial cells invasion and angiogenesis induced by activated platelets
title_fullStr Curcumin suppresses intestinal microvascular endothelial cells invasion and angiogenesis induced by activated platelets
title_full_unstemmed Curcumin suppresses intestinal microvascular endothelial cells invasion and angiogenesis induced by activated platelets
title_short Curcumin suppresses intestinal microvascular endothelial cells invasion and angiogenesis induced by activated platelets
title_sort curcumin suppresses intestinal microvascular endothelial cells invasion and angiogenesis induced by activated platelets
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6601414/
https://www.ncbi.nlm.nih.gov/pubmed/31316605
http://dx.doi.org/10.3892/etm.2019.7662
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