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Detection and quantification of free sulfhydryls in monoclonal antibodies using maleimide labeling and mass spectrometry
The detection of free sulfhydryls in proteins can reveal incomplete disulfide bond formation, indicate cysteine residues available for conjugation, and offer insights into protein stability and structure. Traditional spectroscopic methods of free sulfhydryl detection, such as Ellman’s reagent, gener...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Taylor & Francis
2019
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6601545/ https://www.ncbi.nlm.nih.gov/pubmed/30894096 http://dx.doi.org/10.1080/19420862.2019.1595307 |