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The structure of the yeast Ctf3 complex

Kinetochores are the chromosomal attachment points for spindle microtubules. They are also signaling hubs that control major cell cycle transitions and coordinate chromosome folding. Most well-studied eukaryotes rely on a conserved set of factors, which are divided among two loosely-defined groups,...

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Detalles Bibliográficos
Autores principales: Hinshaw, Stephen M, Dates, Andrew N, Harrison, Stephen C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6602579/
https://www.ncbi.nlm.nih.gov/pubmed/31194673
http://dx.doi.org/10.7554/eLife.48215
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author Hinshaw, Stephen M
Dates, Andrew N
Harrison, Stephen C
author_facet Hinshaw, Stephen M
Dates, Andrew N
Harrison, Stephen C
author_sort Hinshaw, Stephen M
collection PubMed
description Kinetochores are the chromosomal attachment points for spindle microtubules. They are also signaling hubs that control major cell cycle transitions and coordinate chromosome folding. Most well-studied eukaryotes rely on a conserved set of factors, which are divided among two loosely-defined groups, for these functions. Outer kinetochore proteins contact microtubules or regulate this contact directly. Inner kinetochore proteins designate the kinetochore assembly site by recognizing a specialized nucleosome containing the H3 variant Cse4/CENP-A. We previously determined the structure, resolved by cryo-electron microscopy (cryo-EM), of the yeast Ctf19 complex (Ctf19c, homologous to the vertebrate CCAN), providing a high-resolution view of inner kinetochore architecture (Hinshaw and Harrison, 2019). We now extend these observations by reporting a near-atomic model of the Ctf3 complex, the outermost Ctf19c sub-assembly seen in our original cryo-EM density. The model is sufficiently well-determined by the new data to enable molecular interpretation of Ctf3 recruitment and function.
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spelling pubmed-66025792019-07-03 The structure of the yeast Ctf3 complex Hinshaw, Stephen M Dates, Andrew N Harrison, Stephen C eLife Structural Biology and Molecular Biophysics Kinetochores are the chromosomal attachment points for spindle microtubules. They are also signaling hubs that control major cell cycle transitions and coordinate chromosome folding. Most well-studied eukaryotes rely on a conserved set of factors, which are divided among two loosely-defined groups, for these functions. Outer kinetochore proteins contact microtubules or regulate this contact directly. Inner kinetochore proteins designate the kinetochore assembly site by recognizing a specialized nucleosome containing the H3 variant Cse4/CENP-A. We previously determined the structure, resolved by cryo-electron microscopy (cryo-EM), of the yeast Ctf19 complex (Ctf19c, homologous to the vertebrate CCAN), providing a high-resolution view of inner kinetochore architecture (Hinshaw and Harrison, 2019). We now extend these observations by reporting a near-atomic model of the Ctf3 complex, the outermost Ctf19c sub-assembly seen in our original cryo-EM density. The model is sufficiently well-determined by the new data to enable molecular interpretation of Ctf3 recruitment and function. eLife Sciences Publications, Ltd 2019-06-13 /pmc/articles/PMC6602579/ /pubmed/31194673 http://dx.doi.org/10.7554/eLife.48215 Text en © 2019, Hinshaw et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Structural Biology and Molecular Biophysics
Hinshaw, Stephen M
Dates, Andrew N
Harrison, Stephen C
The structure of the yeast Ctf3 complex
title The structure of the yeast Ctf3 complex
title_full The structure of the yeast Ctf3 complex
title_fullStr The structure of the yeast Ctf3 complex
title_full_unstemmed The structure of the yeast Ctf3 complex
title_short The structure of the yeast Ctf3 complex
title_sort structure of the yeast ctf3 complex
topic Structural Biology and Molecular Biophysics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6602579/
https://www.ncbi.nlm.nih.gov/pubmed/31194673
http://dx.doi.org/10.7554/eLife.48215
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