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Transcriptomes of antigen presenting cells in human thymus
Antigen presenting cells (APCs) in the thymus play an essential role in the establishment of central tolerance, i.e. the generation of a repertoire of functional and self-tolerant T cells to prevent autoimmunity. In this study, we have compared the transcriptomes of four primary APCs from human thym...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6602790/ https://www.ncbi.nlm.nih.gov/pubmed/31261375 http://dx.doi.org/10.1371/journal.pone.0218858 |
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author | Gabrielsen, Ingvild S. M. Helgeland, Hanna Akselsen, Helle D. Aass, Hans Christian Sundaram, Arvind Y. M. Snowhite, Isaac V. Pugliese, Alberto Flåm, Siri T. Lie, Benedicte A. |
author_facet | Gabrielsen, Ingvild S. M. Helgeland, Hanna Akselsen, Helle D. Aass, Hans Christian Sundaram, Arvind Y. M. Snowhite, Isaac V. Pugliese, Alberto Flåm, Siri T. Lie, Benedicte A. |
author_sort | Gabrielsen, Ingvild S. M. |
collection | PubMed |
description | Antigen presenting cells (APCs) in the thymus play an essential role in the establishment of central tolerance, i.e. the generation of a repertoire of functional and self-tolerant T cells to prevent autoimmunity. In this study, we have compared the transcriptomes of four primary APCs from human thymus (mTECs, CD19(+) B cells, CD141(+) and CD123(+) DCs). We investigated a set of genes including the HLA genes, genes encoding transcriptional regulators and finally, tissue-enriched genes, i.e, genes with a five-fold higher expression in a particular human tissue. We show that thymic CD141(+) DCs express the highest levels of all classical HLA genes and 67% (14/21) of the HLA class I and II pathway genes investigated in this study. CD141(+) DCs also expressed the highest levels of the transcriptional regulator DEAF1, whereas AIRE and FEZF2 expression were mainly found in primary human mTECs. We found expression of “tissue enriched genes” from the Human Protein Atlas (HPA) in all four APC types, but the mTECs were clearly dominating in the number of uniquely expressed tissue enriched genes (20% in mTECs, 7% in CD19(+) B cells, 4% in CD123(+) DCs and 2% in CD141(+) DCs). The tissue enriched genes also overlapped with reported human autoantigens. This is, to our knowledge, the first study that performs RNA sequencing of mTECs, CD19(+) B cells, CD141(+) and CD123(+) DCs isolated from the same individuals and provides insight into the transcriptomes of these human thymic APCs. |
format | Online Article Text |
id | pubmed-6602790 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-66027902019-07-12 Transcriptomes of antigen presenting cells in human thymus Gabrielsen, Ingvild S. M. Helgeland, Hanna Akselsen, Helle D. Aass, Hans Christian Sundaram, Arvind Y. M. Snowhite, Isaac V. Pugliese, Alberto Flåm, Siri T. Lie, Benedicte A. PLoS One Research Article Antigen presenting cells (APCs) in the thymus play an essential role in the establishment of central tolerance, i.e. the generation of a repertoire of functional and self-tolerant T cells to prevent autoimmunity. In this study, we have compared the transcriptomes of four primary APCs from human thymus (mTECs, CD19(+) B cells, CD141(+) and CD123(+) DCs). We investigated a set of genes including the HLA genes, genes encoding transcriptional regulators and finally, tissue-enriched genes, i.e, genes with a five-fold higher expression in a particular human tissue. We show that thymic CD141(+) DCs express the highest levels of all classical HLA genes and 67% (14/21) of the HLA class I and II pathway genes investigated in this study. CD141(+) DCs also expressed the highest levels of the transcriptional regulator DEAF1, whereas AIRE and FEZF2 expression were mainly found in primary human mTECs. We found expression of “tissue enriched genes” from the Human Protein Atlas (HPA) in all four APC types, but the mTECs were clearly dominating in the number of uniquely expressed tissue enriched genes (20% in mTECs, 7% in CD19(+) B cells, 4% in CD123(+) DCs and 2% in CD141(+) DCs). The tissue enriched genes also overlapped with reported human autoantigens. This is, to our knowledge, the first study that performs RNA sequencing of mTECs, CD19(+) B cells, CD141(+) and CD123(+) DCs isolated from the same individuals and provides insight into the transcriptomes of these human thymic APCs. Public Library of Science 2019-07-01 /pmc/articles/PMC6602790/ /pubmed/31261375 http://dx.doi.org/10.1371/journal.pone.0218858 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication. |
spellingShingle | Research Article Gabrielsen, Ingvild S. M. Helgeland, Hanna Akselsen, Helle D. Aass, Hans Christian Sundaram, Arvind Y. M. Snowhite, Isaac V. Pugliese, Alberto Flåm, Siri T. Lie, Benedicte A. Transcriptomes of antigen presenting cells in human thymus |
title | Transcriptomes of antigen presenting cells in human thymus |
title_full | Transcriptomes of antigen presenting cells in human thymus |
title_fullStr | Transcriptomes of antigen presenting cells in human thymus |
title_full_unstemmed | Transcriptomes of antigen presenting cells in human thymus |
title_short | Transcriptomes of antigen presenting cells in human thymus |
title_sort | transcriptomes of antigen presenting cells in human thymus |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6602790/ https://www.ncbi.nlm.nih.gov/pubmed/31261375 http://dx.doi.org/10.1371/journal.pone.0218858 |
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