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Positive expression of basic transcription factor 3 predicts poor survival of colorectal cancer patients: possible mechanisms involved

Basic transcription factor 3 (BTF3) is associated with the development of several cancers. The aim of our study was to elucidate the role of BTF3 in colorectal cancer (CRC) tissues. CRC tissues or their paired adjacent noncancerous (ANCT) tissues were obtained from 90 patients who underwent operatio...

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Autores principales: Liu, Qi, Wu, Junjie, Lu, Tailiang, Fang, Zhixue, Huang, Zixuan, Lu, Shanzheng, Dai, Chen, Li, Mengqian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6603001/
https://www.ncbi.nlm.nih.gov/pubmed/31263147
http://dx.doi.org/10.1038/s41419-019-1747-2
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author Liu, Qi
Wu, Junjie
Lu, Tailiang
Fang, Zhixue
Huang, Zixuan
Lu, Shanzheng
Dai, Chen
Li, Mengqian
author_facet Liu, Qi
Wu, Junjie
Lu, Tailiang
Fang, Zhixue
Huang, Zixuan
Lu, Shanzheng
Dai, Chen
Li, Mengqian
author_sort Liu, Qi
collection PubMed
description Basic transcription factor 3 (BTF3) is associated with the development of several cancers. The aim of our study was to elucidate the role of BTF3 in colorectal cancer (CRC) tissues. CRC tissues or their paired adjacent noncancerous (ANCT) tissues were obtained from 90 patients who underwent operations in our hospital from November 2011 to December 2016, and then we implemented a gene microarray assay for detecting significant changes in gene expression and confirmed expression in tissues using immunohistochemistry and real-time PCR. We transfected or injected the silencing BTF3 (BTF3-siRNA) plasmid into cells and nude mice, and measured the tumorigenicity of CRC cells with flow cytometry and studied the expression level of BTF3 downstream genes (MAD2L2, MCM3 and PLK1) in CRC cells. BTF3 expression level was not only significantly higher in CRC tissue than in ANCT tissue (2.61 ± 0.07 vs 1.90 ± 0.03, P < 0.001) but BTF3-siRNA decreased tumor formation in a nude mice model. Furthermore, based on the data of gene microarray analysis, MAD2L2, MCM3 and PLK1 were detected as the downstream target genes of BTF3 and their expressions were positive related with BTF3 expression. Also, through transfecting BTF3-siRNA into HCT116 cells, we found that BTF3-siRNA could decrease cell viability and induced cell apoptosis and blocking the cell cycle. In conclusion, BTF3 is positively related to CRC and BTF3-siRNA attenuated the tumorigenicity of colorectal cancer cells via MAD2L2, MCM3 and PLK1 activity reduction.
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spelling pubmed-66030012019-07-02 Positive expression of basic transcription factor 3 predicts poor survival of colorectal cancer patients: possible mechanisms involved Liu, Qi Wu, Junjie Lu, Tailiang Fang, Zhixue Huang, Zixuan Lu, Shanzheng Dai, Chen Li, Mengqian Cell Death Dis Article Basic transcription factor 3 (BTF3) is associated with the development of several cancers. The aim of our study was to elucidate the role of BTF3 in colorectal cancer (CRC) tissues. CRC tissues or their paired adjacent noncancerous (ANCT) tissues were obtained from 90 patients who underwent operations in our hospital from November 2011 to December 2016, and then we implemented a gene microarray assay for detecting significant changes in gene expression and confirmed expression in tissues using immunohistochemistry and real-time PCR. We transfected or injected the silencing BTF3 (BTF3-siRNA) plasmid into cells and nude mice, and measured the tumorigenicity of CRC cells with flow cytometry and studied the expression level of BTF3 downstream genes (MAD2L2, MCM3 and PLK1) in CRC cells. BTF3 expression level was not only significantly higher in CRC tissue than in ANCT tissue (2.61 ± 0.07 vs 1.90 ± 0.03, P < 0.001) but BTF3-siRNA decreased tumor formation in a nude mice model. Furthermore, based on the data of gene microarray analysis, MAD2L2, MCM3 and PLK1 were detected as the downstream target genes of BTF3 and their expressions were positive related with BTF3 expression. Also, through transfecting BTF3-siRNA into HCT116 cells, we found that BTF3-siRNA could decrease cell viability and induced cell apoptosis and blocking the cell cycle. In conclusion, BTF3 is positively related to CRC and BTF3-siRNA attenuated the tumorigenicity of colorectal cancer cells via MAD2L2, MCM3 and PLK1 activity reduction. Nature Publishing Group UK 2019-07-01 /pmc/articles/PMC6603001/ /pubmed/31263147 http://dx.doi.org/10.1038/s41419-019-1747-2 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Liu, Qi
Wu, Junjie
Lu, Tailiang
Fang, Zhixue
Huang, Zixuan
Lu, Shanzheng
Dai, Chen
Li, Mengqian
Positive expression of basic transcription factor 3 predicts poor survival of colorectal cancer patients: possible mechanisms involved
title Positive expression of basic transcription factor 3 predicts poor survival of colorectal cancer patients: possible mechanisms involved
title_full Positive expression of basic transcription factor 3 predicts poor survival of colorectal cancer patients: possible mechanisms involved
title_fullStr Positive expression of basic transcription factor 3 predicts poor survival of colorectal cancer patients: possible mechanisms involved
title_full_unstemmed Positive expression of basic transcription factor 3 predicts poor survival of colorectal cancer patients: possible mechanisms involved
title_short Positive expression of basic transcription factor 3 predicts poor survival of colorectal cancer patients: possible mechanisms involved
title_sort positive expression of basic transcription factor 3 predicts poor survival of colorectal cancer patients: possible mechanisms involved
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6603001/
https://www.ncbi.nlm.nih.gov/pubmed/31263147
http://dx.doi.org/10.1038/s41419-019-1747-2
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