Cargando…

Preparation of iron oxide mesoporous magnetic microparticles as novel multidrug carriers for synergistic anticancer therapy and deep tumor penetration

The preparation of mesoporous iron oxides with controllable physiochemical properties for effective therapeutic drug delivery remains a formidable challenge. Herein, iron oxide mesoporous magnetic microparticles (IO-MMMs) were prepared by a modified reverse hard-templating approach using, for the fi...

Descripción completa

Detalles Bibliográficos
Autores principales: El-Boubbou, Kheireddine, Ali, Rizwan, Al-Zahrani, Hajar, Trivilegio, Thadeo, Alanazi, Abdullah H., Khan, Abdul Latif, Boudjelal, Mohamed, AlKushi, Abdulmohsen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6603044/
https://www.ncbi.nlm.nih.gov/pubmed/31263250
http://dx.doi.org/10.1038/s41598-019-46007-z
_version_ 1783431441955160064
author El-Boubbou, Kheireddine
Ali, Rizwan
Al-Zahrani, Hajar
Trivilegio, Thadeo
Alanazi, Abdullah H.
Khan, Abdul Latif
Boudjelal, Mohamed
AlKushi, Abdulmohsen
author_facet El-Boubbou, Kheireddine
Ali, Rizwan
Al-Zahrani, Hajar
Trivilegio, Thadeo
Alanazi, Abdullah H.
Khan, Abdul Latif
Boudjelal, Mohamed
AlKushi, Abdulmohsen
author_sort El-Boubbou, Kheireddine
collection PubMed
description The preparation of mesoporous iron oxides with controllable physiochemical properties for effective therapeutic drug delivery remains a formidable challenge. Herein, iron oxide mesoporous magnetic microparticles (IO-MMMs) were prepared by a modified reverse hard-templating approach using, for the first time, acid-prepared mesoporous spheres (APMS) as the hard silica template. The obtained mesostructures exhibited remarkably high surface area and large pore volumes (S(BET) = 240 m(2)/g and V(pore) = 0.55 cm(3)/g), controllable average sizes, generally uniform morphologies, and excellent biocompatibilities, allowing them to achieve optimal drug release in cancer cells and tumor tissues. IO-MMM carriers were able to co-load high amounts of hydrophilic chemotherapeutic drugs (Dox or Daun) and/or hydrophobic hormonal anticancer drugs (Tam), and release them sustainably in a pH-dependent manner, utilizing the fluorescence of Daun to real-time trace the intracellular drug distribution, and employing Daun/Tam to treat cancer by combined chemo/hormonal therapy. Cytotoxicity assays against different types of cancerous cells showed that the combinatory Daun/Tam@IO-MMM formulation significantly reduced the viability of metastatic MCF7 and KAIMRC1 breast as well as HCT8 colorectal cancer cells, with the least potency towards non-cancerous normal primary cells (up to 10-fold). Electron, flow, and live confocal microscopy imaging confirmed that the loaded vehicles were successfully and differentially uptaken by the different tested cells, gradually releasing their payloads, and causing apoptotic cell death. Importantly, compared to free drugs, Daun/Tam@IO-MMMs displayed enhanced drug accumulation in patient breast primary tumor tissues, deeply penetrating into the tumor region and killing the tumor cells inside. The designed carriers described here, thus, constitute a novel promising magnetic mesoporous smart system that entraps different kinds of drugs and release them in a controlled manner for combinatorial chemo/hormonal cancer theranostics. This multifactorial platform may open new avenues in cancer therapy as efficient synergistic antitumor system through overcoming limitations of conventional cancer therapy.
format Online
Article
Text
id pubmed-6603044
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-66030442019-07-14 Preparation of iron oxide mesoporous magnetic microparticles as novel multidrug carriers for synergistic anticancer therapy and deep tumor penetration El-Boubbou, Kheireddine Ali, Rizwan Al-Zahrani, Hajar Trivilegio, Thadeo Alanazi, Abdullah H. Khan, Abdul Latif Boudjelal, Mohamed AlKushi, Abdulmohsen Sci Rep Article The preparation of mesoporous iron oxides with controllable physiochemical properties for effective therapeutic drug delivery remains a formidable challenge. Herein, iron oxide mesoporous magnetic microparticles (IO-MMMs) were prepared by a modified reverse hard-templating approach using, for the first time, acid-prepared mesoporous spheres (APMS) as the hard silica template. The obtained mesostructures exhibited remarkably high surface area and large pore volumes (S(BET) = 240 m(2)/g and V(pore) = 0.55 cm(3)/g), controllable average sizes, generally uniform morphologies, and excellent biocompatibilities, allowing them to achieve optimal drug release in cancer cells and tumor tissues. IO-MMM carriers were able to co-load high amounts of hydrophilic chemotherapeutic drugs (Dox or Daun) and/or hydrophobic hormonal anticancer drugs (Tam), and release them sustainably in a pH-dependent manner, utilizing the fluorescence of Daun to real-time trace the intracellular drug distribution, and employing Daun/Tam to treat cancer by combined chemo/hormonal therapy. Cytotoxicity assays against different types of cancerous cells showed that the combinatory Daun/Tam@IO-MMM formulation significantly reduced the viability of metastatic MCF7 and KAIMRC1 breast as well as HCT8 colorectal cancer cells, with the least potency towards non-cancerous normal primary cells (up to 10-fold). Electron, flow, and live confocal microscopy imaging confirmed that the loaded vehicles were successfully and differentially uptaken by the different tested cells, gradually releasing their payloads, and causing apoptotic cell death. Importantly, compared to free drugs, Daun/Tam@IO-MMMs displayed enhanced drug accumulation in patient breast primary tumor tissues, deeply penetrating into the tumor region and killing the tumor cells inside. The designed carriers described here, thus, constitute a novel promising magnetic mesoporous smart system that entraps different kinds of drugs and release them in a controlled manner for combinatorial chemo/hormonal cancer theranostics. This multifactorial platform may open new avenues in cancer therapy as efficient synergistic antitumor system through overcoming limitations of conventional cancer therapy. Nature Publishing Group UK 2019-07-01 /pmc/articles/PMC6603044/ /pubmed/31263250 http://dx.doi.org/10.1038/s41598-019-46007-z Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
El-Boubbou, Kheireddine
Ali, Rizwan
Al-Zahrani, Hajar
Trivilegio, Thadeo
Alanazi, Abdullah H.
Khan, Abdul Latif
Boudjelal, Mohamed
AlKushi, Abdulmohsen
Preparation of iron oxide mesoporous magnetic microparticles as novel multidrug carriers for synergistic anticancer therapy and deep tumor penetration
title Preparation of iron oxide mesoporous magnetic microparticles as novel multidrug carriers for synergistic anticancer therapy and deep tumor penetration
title_full Preparation of iron oxide mesoporous magnetic microparticles as novel multidrug carriers for synergistic anticancer therapy and deep tumor penetration
title_fullStr Preparation of iron oxide mesoporous magnetic microparticles as novel multidrug carriers for synergistic anticancer therapy and deep tumor penetration
title_full_unstemmed Preparation of iron oxide mesoporous magnetic microparticles as novel multidrug carriers for synergistic anticancer therapy and deep tumor penetration
title_short Preparation of iron oxide mesoporous magnetic microparticles as novel multidrug carriers for synergistic anticancer therapy and deep tumor penetration
title_sort preparation of iron oxide mesoporous magnetic microparticles as novel multidrug carriers for synergistic anticancer therapy and deep tumor penetration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6603044/
https://www.ncbi.nlm.nih.gov/pubmed/31263250
http://dx.doi.org/10.1038/s41598-019-46007-z
work_keys_str_mv AT elboubboukheireddine preparationofironoxidemesoporousmagneticmicroparticlesasnovelmultidrugcarriersforsynergisticanticancertherapyanddeeptumorpenetration
AT alirizwan preparationofironoxidemesoporousmagneticmicroparticlesasnovelmultidrugcarriersforsynergisticanticancertherapyanddeeptumorpenetration
AT alzahranihajar preparationofironoxidemesoporousmagneticmicroparticlesasnovelmultidrugcarriersforsynergisticanticancertherapyanddeeptumorpenetration
AT trivilegiothadeo preparationofironoxidemesoporousmagneticmicroparticlesasnovelmultidrugcarriersforsynergisticanticancertherapyanddeeptumorpenetration
AT alanaziabdullahh preparationofironoxidemesoporousmagneticmicroparticlesasnovelmultidrugcarriersforsynergisticanticancertherapyanddeeptumorpenetration
AT khanabdullatif preparationofironoxidemesoporousmagneticmicroparticlesasnovelmultidrugcarriersforsynergisticanticancertherapyanddeeptumorpenetration
AT boudjelalmohamed preparationofironoxidemesoporousmagneticmicroparticlesasnovelmultidrugcarriersforsynergisticanticancertherapyanddeeptumorpenetration
AT alkushiabdulmohsen preparationofironoxidemesoporousmagneticmicroparticlesasnovelmultidrugcarriersforsynergisticanticancertherapyanddeeptumorpenetration