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Simultaneous Measurement of Neuronal Activity in the Pontine Micturition Center and Cystometry in Freely Moving Mice

Understanding the complex neural mechanisms controlling urinary bladder activity is an extremely important topic in both neuroscience and urology. Simultaneously recording of the bladder activity and neural activity in related brain regions will largely advance this field. However, such recording ap...

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Autores principales: Yao, Jiwei, Li, Qianwei, Li, Xianping, Qin, Han, Liang, Shanshan, Liao, Xiang, Chen, Xiaowei, Li, Weibing, Yan, Junan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6603236/
https://www.ncbi.nlm.nih.gov/pubmed/31293380
http://dx.doi.org/10.3389/fnins.2019.00663
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author Yao, Jiwei
Li, Qianwei
Li, Xianping
Qin, Han
Liang, Shanshan
Liao, Xiang
Chen, Xiaowei
Li, Weibing
Yan, Junan
author_facet Yao, Jiwei
Li, Qianwei
Li, Xianping
Qin, Han
Liang, Shanshan
Liao, Xiang
Chen, Xiaowei
Li, Weibing
Yan, Junan
author_sort Yao, Jiwei
collection PubMed
description Understanding the complex neural mechanisms controlling urinary bladder activity is an extremely important topic in both neuroscience and urology. Simultaneously recording of the bladder activity and neural activity in related brain regions will largely advance this field. However, such recording approach has long been restricted to anesthetized animals, whose bladder function and urodynamic properties are largely affected by anesthetics. In our recent report, we found that it is feasible to record bladder pressure (cystometry) and the related cortical neuron activity simultaneously in freely moving mice. Here, we aimed to demonstrate the use of this combined method in freely moving mice for recording the activity of the pontine micturition center (PMC), a more difficultly approachable small region deeply located in the brainstem and a more popularly studied hub for controlling bladder function. Interestingly, we found that the duration of urination events linearly correlated to the time course of neuronal activity in the PMC. We observed that the activities of PMC neurons highly correlated with spike-like increases in bladder pressure, reflecting bladder contractions. We also found that anesthesia evoked prominent changes in the dynamics of the Ca(2+) signals in the PMC during the bladder contraction and even induced the dripping overflow incontinence due to suppression of the neural activity in the PMC. In addition, we described in details both the system for cystometry in freely moving mice and the protocols for how to perform this combined method. Therefore, this work provides a powerful approach that enables the simultaneous measurement of neuronal activity of the PMC or any other brain sites and bladder function in freely behaving mice. This approach offers a promising possibility to examine the neural mechanisms underlying neurogenic bladder dysfunction.
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spelling pubmed-66032362019-07-10 Simultaneous Measurement of Neuronal Activity in the Pontine Micturition Center and Cystometry in Freely Moving Mice Yao, Jiwei Li, Qianwei Li, Xianping Qin, Han Liang, Shanshan Liao, Xiang Chen, Xiaowei Li, Weibing Yan, Junan Front Neurosci Neuroscience Understanding the complex neural mechanisms controlling urinary bladder activity is an extremely important topic in both neuroscience and urology. Simultaneously recording of the bladder activity and neural activity in related brain regions will largely advance this field. However, such recording approach has long been restricted to anesthetized animals, whose bladder function and urodynamic properties are largely affected by anesthetics. In our recent report, we found that it is feasible to record bladder pressure (cystometry) and the related cortical neuron activity simultaneously in freely moving mice. Here, we aimed to demonstrate the use of this combined method in freely moving mice for recording the activity of the pontine micturition center (PMC), a more difficultly approachable small region deeply located in the brainstem and a more popularly studied hub for controlling bladder function. Interestingly, we found that the duration of urination events linearly correlated to the time course of neuronal activity in the PMC. We observed that the activities of PMC neurons highly correlated with spike-like increases in bladder pressure, reflecting bladder contractions. We also found that anesthesia evoked prominent changes in the dynamics of the Ca(2+) signals in the PMC during the bladder contraction and even induced the dripping overflow incontinence due to suppression of the neural activity in the PMC. In addition, we described in details both the system for cystometry in freely moving mice and the protocols for how to perform this combined method. Therefore, this work provides a powerful approach that enables the simultaneous measurement of neuronal activity of the PMC or any other brain sites and bladder function in freely behaving mice. This approach offers a promising possibility to examine the neural mechanisms underlying neurogenic bladder dysfunction. Frontiers Media S.A. 2019-06-25 /pmc/articles/PMC6603236/ /pubmed/31293380 http://dx.doi.org/10.3389/fnins.2019.00663 Text en Copyright © 2019 Yao, Li, Li, Qin, Liang, Liao, Chen, Li and Yan. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Yao, Jiwei
Li, Qianwei
Li, Xianping
Qin, Han
Liang, Shanshan
Liao, Xiang
Chen, Xiaowei
Li, Weibing
Yan, Junan
Simultaneous Measurement of Neuronal Activity in the Pontine Micturition Center and Cystometry in Freely Moving Mice
title Simultaneous Measurement of Neuronal Activity in the Pontine Micturition Center and Cystometry in Freely Moving Mice
title_full Simultaneous Measurement of Neuronal Activity in the Pontine Micturition Center and Cystometry in Freely Moving Mice
title_fullStr Simultaneous Measurement of Neuronal Activity in the Pontine Micturition Center and Cystometry in Freely Moving Mice
title_full_unstemmed Simultaneous Measurement of Neuronal Activity in the Pontine Micturition Center and Cystometry in Freely Moving Mice
title_short Simultaneous Measurement of Neuronal Activity in the Pontine Micturition Center and Cystometry in Freely Moving Mice
title_sort simultaneous measurement of neuronal activity in the pontine micturition center and cystometry in freely moving mice
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6603236/
https://www.ncbi.nlm.nih.gov/pubmed/31293380
http://dx.doi.org/10.3389/fnins.2019.00663
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