Cargando…

Netrin-1 promotes cell neural invasion in gastric cancer via its receptor neogenin

Neural invasion (NI) is one of the important routes for local spread of gastric cancer (GC) correlated with poor prognosis. However, the exact cellular characteristics and molecular mechanisms of NI in GC are still unclear. Netrin-1(NTN1) as an axon guidance molecule was firstly found during neural...

Descripción completa

Detalles Bibliográficos
Autores principales: Yin, Kai, Wang, Linjun, Xia, Yiwen, Dang, Shengchun, Zhang, Xuan, He, Zhongyuan, Xu, Jianghao, Shang, Mengyuan, Xu, Zekuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6603376/
https://www.ncbi.nlm.nih.gov/pubmed/31289590
http://dx.doi.org/10.7150/jca.30230
_version_ 1783431504174514176
author Yin, Kai
Wang, Linjun
Xia, Yiwen
Dang, Shengchun
Zhang, Xuan
He, Zhongyuan
Xu, Jianghao
Shang, Mengyuan
Xu, Zekuan
author_facet Yin, Kai
Wang, Linjun
Xia, Yiwen
Dang, Shengchun
Zhang, Xuan
He, Zhongyuan
Xu, Jianghao
Shang, Mengyuan
Xu, Zekuan
author_sort Yin, Kai
collection PubMed
description Neural invasion (NI) is one of the important routes for local spread of gastric cancer (GC) correlated with poor prognosis. However, the exact cellular characteristics and molecular mechanisms of NI in GC are still unclear. Netrin-1(NTN1) as an axon guidance molecule was firstly found during neural system development. Importantly, NTN1 has an essential role in the progression of malignant tumor and specifically mediates the induction of invasion. In this study, we found NTN1 expression was significantly increased in 97 tumor tissues from GC patients and positively correlated with NI (p<0.05). In addition, we detected NTN1 knockdown significantly suppressed GC cells migration and invasion. Moreover, our results showed that reciprocity was observed between GC cells and neurites colonies in dorsal root ganglia (DRG)-GC cells co-culture vitro model. GC cells with NTN1 silencing could suppress their abilities to navigate along surrounding neuritis and this effect was depended on its receptor neogenin. In vivo, NTN1 inhibition also decreased GC cells sciatic nerve invasion. Taken together, our findings argue that NTN1 and its receptor neogenin might act synergistically in promoting GC cells neural invasion. Inhibiting the activity of NTN1 could be a potential strategy targeting NI in GC therapy.
format Online
Article
Text
id pubmed-6603376
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Ivyspring International Publisher
record_format MEDLINE/PubMed
spelling pubmed-66033762019-07-09 Netrin-1 promotes cell neural invasion in gastric cancer via its receptor neogenin Yin, Kai Wang, Linjun Xia, Yiwen Dang, Shengchun Zhang, Xuan He, Zhongyuan Xu, Jianghao Shang, Mengyuan Xu, Zekuan J Cancer Research Paper Neural invasion (NI) is one of the important routes for local spread of gastric cancer (GC) correlated with poor prognosis. However, the exact cellular characteristics and molecular mechanisms of NI in GC are still unclear. Netrin-1(NTN1) as an axon guidance molecule was firstly found during neural system development. Importantly, NTN1 has an essential role in the progression of malignant tumor and specifically mediates the induction of invasion. In this study, we found NTN1 expression was significantly increased in 97 tumor tissues from GC patients and positively correlated with NI (p<0.05). In addition, we detected NTN1 knockdown significantly suppressed GC cells migration and invasion. Moreover, our results showed that reciprocity was observed between GC cells and neurites colonies in dorsal root ganglia (DRG)-GC cells co-culture vitro model. GC cells with NTN1 silencing could suppress their abilities to navigate along surrounding neuritis and this effect was depended on its receptor neogenin. In vivo, NTN1 inhibition also decreased GC cells sciatic nerve invasion. Taken together, our findings argue that NTN1 and its receptor neogenin might act synergistically in promoting GC cells neural invasion. Inhibiting the activity of NTN1 could be a potential strategy targeting NI in GC therapy. Ivyspring International Publisher 2019-06-02 /pmc/articles/PMC6603376/ /pubmed/31289590 http://dx.doi.org/10.7150/jca.30230 Text en © Ivyspring International Publisher This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Yin, Kai
Wang, Linjun
Xia, Yiwen
Dang, Shengchun
Zhang, Xuan
He, Zhongyuan
Xu, Jianghao
Shang, Mengyuan
Xu, Zekuan
Netrin-1 promotes cell neural invasion in gastric cancer via its receptor neogenin
title Netrin-1 promotes cell neural invasion in gastric cancer via its receptor neogenin
title_full Netrin-1 promotes cell neural invasion in gastric cancer via its receptor neogenin
title_fullStr Netrin-1 promotes cell neural invasion in gastric cancer via its receptor neogenin
title_full_unstemmed Netrin-1 promotes cell neural invasion in gastric cancer via its receptor neogenin
title_short Netrin-1 promotes cell neural invasion in gastric cancer via its receptor neogenin
title_sort netrin-1 promotes cell neural invasion in gastric cancer via its receptor neogenin
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6603376/
https://www.ncbi.nlm.nih.gov/pubmed/31289590
http://dx.doi.org/10.7150/jca.30230
work_keys_str_mv AT yinkai netrin1promotescellneuralinvasioningastriccancerviaitsreceptorneogenin
AT wanglinjun netrin1promotescellneuralinvasioningastriccancerviaitsreceptorneogenin
AT xiayiwen netrin1promotescellneuralinvasioningastriccancerviaitsreceptorneogenin
AT dangshengchun netrin1promotescellneuralinvasioningastriccancerviaitsreceptorneogenin
AT zhangxuan netrin1promotescellneuralinvasioningastriccancerviaitsreceptorneogenin
AT hezhongyuan netrin1promotescellneuralinvasioningastriccancerviaitsreceptorneogenin
AT xujianghao netrin1promotescellneuralinvasioningastriccancerviaitsreceptorneogenin
AT shangmengyuan netrin1promotescellneuralinvasioningastriccancerviaitsreceptorneogenin
AT xuzekuan netrin1promotescellneuralinvasioningastriccancerviaitsreceptorneogenin