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Perfusion Microfermentor Integrated into a Fiber Optic Quasi-Elastic Light Scattering Sensor for Fast Screening of Microbial Growth Parameters
This research presents a microfermentor integrated into an optical fiber sensor based on quasi-elastic light scattering (QELS) to monitor and swiftly identify cellular growth kinetic parameters. The system uses a 1310 nm laser light that is guided through single-mode silica optical fibers to the int...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6603560/ https://www.ncbi.nlm.nih.gov/pubmed/31159228 http://dx.doi.org/10.3390/s19112493 |
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author | Soares, Marco César Prado Vit, Franciele Flores Suzuki, Carlos Kenichi de la Torre, Lucimara Gaziola Fujiwara, Eric |
author_facet | Soares, Marco César Prado Vit, Franciele Flores Suzuki, Carlos Kenichi de la Torre, Lucimara Gaziola Fujiwara, Eric |
author_sort | Soares, Marco César Prado |
collection | PubMed |
description | This research presents a microfermentor integrated into an optical fiber sensor based on quasi-elastic light scattering (QELS) to monitor and swiftly identify cellular growth kinetic parameters. The system uses a 1310 nm laser light that is guided through single-mode silica optical fibers to the interior of perfusion chambers, which are separated by polycarbonate membranes (470 nm pores) from microchannels, where a culture medium flows in a constant concentration. The system contains four layers, a superior and an inferior layer made of glass, and two intermediate poly(dimethylsiloxane) layers that contain the microchannels and the perfusion chambers, forming a reversible microfluidic device that requires only the sealing of the fibers to the inferior glass cover. The QELS autocorrelation decay rates of the optical signals were correlated to the cells counting in a microscope, and the application of this microsystem to the monitoring of alcoholic fermentation of Saccharomyces cerevisiae resulted in the kinetic parameters of K(M) = 4.1 g/L and μ(m) = 0.49 h(−1). These results agree with both the data reported in the literature and with the control batch test, showing that it is a reliable and efficient biological monitoring system. |
format | Online Article Text |
id | pubmed-6603560 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-66035602019-07-17 Perfusion Microfermentor Integrated into a Fiber Optic Quasi-Elastic Light Scattering Sensor for Fast Screening of Microbial Growth Parameters Soares, Marco César Prado Vit, Franciele Flores Suzuki, Carlos Kenichi de la Torre, Lucimara Gaziola Fujiwara, Eric Sensors (Basel) Article This research presents a microfermentor integrated into an optical fiber sensor based on quasi-elastic light scattering (QELS) to monitor and swiftly identify cellular growth kinetic parameters. The system uses a 1310 nm laser light that is guided through single-mode silica optical fibers to the interior of perfusion chambers, which are separated by polycarbonate membranes (470 nm pores) from microchannels, where a culture medium flows in a constant concentration. The system contains four layers, a superior and an inferior layer made of glass, and two intermediate poly(dimethylsiloxane) layers that contain the microchannels and the perfusion chambers, forming a reversible microfluidic device that requires only the sealing of the fibers to the inferior glass cover. The QELS autocorrelation decay rates of the optical signals were correlated to the cells counting in a microscope, and the application of this microsystem to the monitoring of alcoholic fermentation of Saccharomyces cerevisiae resulted in the kinetic parameters of K(M) = 4.1 g/L and μ(m) = 0.49 h(−1). These results agree with both the data reported in the literature and with the control batch test, showing that it is a reliable and efficient biological monitoring system. MDPI 2019-05-31 /pmc/articles/PMC6603560/ /pubmed/31159228 http://dx.doi.org/10.3390/s19112493 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Soares, Marco César Prado Vit, Franciele Flores Suzuki, Carlos Kenichi de la Torre, Lucimara Gaziola Fujiwara, Eric Perfusion Microfermentor Integrated into a Fiber Optic Quasi-Elastic Light Scattering Sensor for Fast Screening of Microbial Growth Parameters |
title | Perfusion Microfermentor Integrated into a Fiber Optic Quasi-Elastic Light Scattering Sensor for Fast Screening of Microbial Growth Parameters |
title_full | Perfusion Microfermentor Integrated into a Fiber Optic Quasi-Elastic Light Scattering Sensor for Fast Screening of Microbial Growth Parameters |
title_fullStr | Perfusion Microfermentor Integrated into a Fiber Optic Quasi-Elastic Light Scattering Sensor for Fast Screening of Microbial Growth Parameters |
title_full_unstemmed | Perfusion Microfermentor Integrated into a Fiber Optic Quasi-Elastic Light Scattering Sensor for Fast Screening of Microbial Growth Parameters |
title_short | Perfusion Microfermentor Integrated into a Fiber Optic Quasi-Elastic Light Scattering Sensor for Fast Screening of Microbial Growth Parameters |
title_sort | perfusion microfermentor integrated into a fiber optic quasi-elastic light scattering sensor for fast screening of microbial growth parameters |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6603560/ https://www.ncbi.nlm.nih.gov/pubmed/31159228 http://dx.doi.org/10.3390/s19112493 |
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