Cargando…

Multiplex Detection of Different Magnetic Beads Using Frequency Scanning in Magnetic Frequency Mixing Technique

In modern bioanalytical methods, it is often desired to detect several targets in one sample within one measurement. Immunological methods including those that use superparamagnetic beads are an important group of techniques for these applications. The goal of this work is to investigate the feasibi...

Descripción completa

Detalles Bibliográficos
Autores principales: Achtsnicht, Stefan, Pourshahidi, Ali Mohammad, Offenhäusser, Andreas, Krause, Hans-Joachim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6603599/
https://www.ncbi.nlm.nih.gov/pubmed/31181672
http://dx.doi.org/10.3390/s19112599
_version_ 1783431542269280256
author Achtsnicht, Stefan
Pourshahidi, Ali Mohammad
Offenhäusser, Andreas
Krause, Hans-Joachim
author_facet Achtsnicht, Stefan
Pourshahidi, Ali Mohammad
Offenhäusser, Andreas
Krause, Hans-Joachim
author_sort Achtsnicht, Stefan
collection PubMed
description In modern bioanalytical methods, it is often desired to detect several targets in one sample within one measurement. Immunological methods including those that use superparamagnetic beads are an important group of techniques for these applications. The goal of this work is to investigate the feasibility of simultaneously detecting different superparamagnetic beads acting as markers using the magnetic frequency mixing technique. The frequency of the magnetic excitation field is scanned while the lower driving frequency is kept constant. Due to the particles’ nonlinear magnetization, mixing frequencies are generated. To record their amplitude and phase information, a direct digitization of the pickup-coil’s signal with subsequent Fast Fourier Transformation is performed. By synchronizing both magnetic fields, a stable phase information is gained. In this research, it is shown that the amplitude of the dominant mixing component is proportional to the amount of superparamagnetic beads inside a sample. Additionally, it is shown that the phase does not show this behaviour. Excitation frequency scans of different bead types were performed, showing different phases, without correlation to their diverse amplitudes. Two commercially available beads were selected and a determination of their amount in a mixture is performed as a demonstration for multiplex measurements.
format Online
Article
Text
id pubmed-6603599
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-66035992019-07-17 Multiplex Detection of Different Magnetic Beads Using Frequency Scanning in Magnetic Frequency Mixing Technique Achtsnicht, Stefan Pourshahidi, Ali Mohammad Offenhäusser, Andreas Krause, Hans-Joachim Sensors (Basel) Article In modern bioanalytical methods, it is often desired to detect several targets in one sample within one measurement. Immunological methods including those that use superparamagnetic beads are an important group of techniques for these applications. The goal of this work is to investigate the feasibility of simultaneously detecting different superparamagnetic beads acting as markers using the magnetic frequency mixing technique. The frequency of the magnetic excitation field is scanned while the lower driving frequency is kept constant. Due to the particles’ nonlinear magnetization, mixing frequencies are generated. To record their amplitude and phase information, a direct digitization of the pickup-coil’s signal with subsequent Fast Fourier Transformation is performed. By synchronizing both magnetic fields, a stable phase information is gained. In this research, it is shown that the amplitude of the dominant mixing component is proportional to the amount of superparamagnetic beads inside a sample. Additionally, it is shown that the phase does not show this behaviour. Excitation frequency scans of different bead types were performed, showing different phases, without correlation to their diverse amplitudes. Two commercially available beads were selected and a determination of their amount in a mixture is performed as a demonstration for multiplex measurements. MDPI 2019-06-07 /pmc/articles/PMC6603599/ /pubmed/31181672 http://dx.doi.org/10.3390/s19112599 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Achtsnicht, Stefan
Pourshahidi, Ali Mohammad
Offenhäusser, Andreas
Krause, Hans-Joachim
Multiplex Detection of Different Magnetic Beads Using Frequency Scanning in Magnetic Frequency Mixing Technique
title Multiplex Detection of Different Magnetic Beads Using Frequency Scanning in Magnetic Frequency Mixing Technique
title_full Multiplex Detection of Different Magnetic Beads Using Frequency Scanning in Magnetic Frequency Mixing Technique
title_fullStr Multiplex Detection of Different Magnetic Beads Using Frequency Scanning in Magnetic Frequency Mixing Technique
title_full_unstemmed Multiplex Detection of Different Magnetic Beads Using Frequency Scanning in Magnetic Frequency Mixing Technique
title_short Multiplex Detection of Different Magnetic Beads Using Frequency Scanning in Magnetic Frequency Mixing Technique
title_sort multiplex detection of different magnetic beads using frequency scanning in magnetic frequency mixing technique
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6603599/
https://www.ncbi.nlm.nih.gov/pubmed/31181672
http://dx.doi.org/10.3390/s19112599
work_keys_str_mv AT achtsnichtstefan multiplexdetectionofdifferentmagneticbeadsusingfrequencyscanninginmagneticfrequencymixingtechnique
AT pourshahidialimohammad multiplexdetectionofdifferentmagneticbeadsusingfrequencyscanninginmagneticfrequencymixingtechnique
AT offenhausserandreas multiplexdetectionofdifferentmagneticbeadsusingfrequencyscanninginmagneticfrequencymixingtechnique
AT krausehansjoachim multiplexdetectionofdifferentmagneticbeadsusingfrequencyscanninginmagneticfrequencymixingtechnique