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Non-Contact Temperature Control System Applicable to Polymerase Chain Reaction on a Lab-on-a-Disc
Polymerase chain reaction (PCR) and the visual inspection of fluorescent amplicons for detection are commonly used procedures in nucleic acid tests. However, it has been extremely challenging to incorporate PCR onto a lab-on-a-disc (PCR–LOD) as it involves controlling the complicated and precise hea...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6603647/ https://www.ncbi.nlm.nih.gov/pubmed/31181849 http://dx.doi.org/10.3390/s19112621 |
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author | Ko, Junguk Yoo, Jae-Chern |
author_facet | Ko, Junguk Yoo, Jae-Chern |
author_sort | Ko, Junguk |
collection | PubMed |
description | Polymerase chain reaction (PCR) and the visual inspection of fluorescent amplicons for detection are commonly used procedures in nucleic acid tests. However, it has been extremely challenging to incorporate PCR onto a lab-on-a-disc (PCR–LOD) as it involves controlling the complicated and precise heating steps during thermal cycling and the measurement of reagent temperature. Additionally, a non-contact temperature control system without any connecting attachments needs to be implemented to facilitate the rotation of the PCR–LOD. This study presents a non-contact temperature control system to integrate conventional PCR onto an LOD. The experimental results demonstrate that our proposed system provides one-stop detection capabilities for Salmonella with a stable PCR amplification in a single PCR–LOD. |
format | Online Article Text |
id | pubmed-6603647 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-66036472019-07-17 Non-Contact Temperature Control System Applicable to Polymerase Chain Reaction on a Lab-on-a-Disc Ko, Junguk Yoo, Jae-Chern Sensors (Basel) Article Polymerase chain reaction (PCR) and the visual inspection of fluorescent amplicons for detection are commonly used procedures in nucleic acid tests. However, it has been extremely challenging to incorporate PCR onto a lab-on-a-disc (PCR–LOD) as it involves controlling the complicated and precise heating steps during thermal cycling and the measurement of reagent temperature. Additionally, a non-contact temperature control system without any connecting attachments needs to be implemented to facilitate the rotation of the PCR–LOD. This study presents a non-contact temperature control system to integrate conventional PCR onto an LOD. The experimental results demonstrate that our proposed system provides one-stop detection capabilities for Salmonella with a stable PCR amplification in a single PCR–LOD. MDPI 2019-06-09 /pmc/articles/PMC6603647/ /pubmed/31181849 http://dx.doi.org/10.3390/s19112621 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ko, Junguk Yoo, Jae-Chern Non-Contact Temperature Control System Applicable to Polymerase Chain Reaction on a Lab-on-a-Disc |
title | Non-Contact Temperature Control System Applicable to Polymerase Chain Reaction on a Lab-on-a-Disc |
title_full | Non-Contact Temperature Control System Applicable to Polymerase Chain Reaction on a Lab-on-a-Disc |
title_fullStr | Non-Contact Temperature Control System Applicable to Polymerase Chain Reaction on a Lab-on-a-Disc |
title_full_unstemmed | Non-Contact Temperature Control System Applicable to Polymerase Chain Reaction on a Lab-on-a-Disc |
title_short | Non-Contact Temperature Control System Applicable to Polymerase Chain Reaction on a Lab-on-a-Disc |
title_sort | non-contact temperature control system applicable to polymerase chain reaction on a lab-on-a-disc |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6603647/ https://www.ncbi.nlm.nih.gov/pubmed/31181849 http://dx.doi.org/10.3390/s19112621 |
work_keys_str_mv | AT kojunguk noncontacttemperaturecontrolsystemapplicabletopolymerasechainreactiononalabonadisc AT yoojaechern noncontacttemperaturecontrolsystemapplicabletopolymerasechainreactiononalabonadisc |