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Unique microglia expression profile in developing white matter

OBJECTIVE: Recently we demonstrated that amoeboid microglia in white matter regions are essential for proper oligodendrocyte homeostasis and myelinogenesis in the first postnatal week. Amoeboid microglia in the mouse corpus callosum change their activation profile within few days after postnatal day...

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Autores principales: Staszewski, Ori, Hagemeyer, Nora
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6604453/
https://www.ncbi.nlm.nih.gov/pubmed/31262353
http://dx.doi.org/10.1186/s13104-019-4410-1
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author Staszewski, Ori
Hagemeyer, Nora
author_facet Staszewski, Ori
Hagemeyer, Nora
author_sort Staszewski, Ori
collection PubMed
description OBJECTIVE: Recently we demonstrated that amoeboid microglia in white matter regions are essential for proper oligodendrocyte homeostasis and myelinogenesis in the first postnatal week. Amoeboid microglia in the mouse corpus callosum change their activation profile within few days after postnatal day (P)7 with microglia of the cerebellum showing similar features. Here we expanded our previous transcriptional analysis and performed detailed bulk RNA sequencing of microglia from corpus callosum, cortex and cerebellum at P7, P10 and P42. The goal of this study was to identify a specific gene profile for both, white matter and grey matter microglia during development. RESULTS: Microglia in white matter regions display unique characteristics in the first postnatal week of murine life. In both the corpus callosum and cerebellum microglia show amoeboid morphology and a similar transcription profile during development including high expression of genes related to priming of microglia, phagocytosis and migration at P7; characteristics which are already lost at P10. Together these data verify our previous transcriptional data obtained by microarray analysis and enable a more complete view into white matter and grey matter microglia at different developmental stages. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13104-019-4410-1) contains supplementary material, which is available to authorized users.
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spelling pubmed-66044532019-07-12 Unique microglia expression profile in developing white matter Staszewski, Ori Hagemeyer, Nora BMC Res Notes Research Note OBJECTIVE: Recently we demonstrated that amoeboid microglia in white matter regions are essential for proper oligodendrocyte homeostasis and myelinogenesis in the first postnatal week. Amoeboid microglia in the mouse corpus callosum change their activation profile within few days after postnatal day (P)7 with microglia of the cerebellum showing similar features. Here we expanded our previous transcriptional analysis and performed detailed bulk RNA sequencing of microglia from corpus callosum, cortex and cerebellum at P7, P10 and P42. The goal of this study was to identify a specific gene profile for both, white matter and grey matter microglia during development. RESULTS: Microglia in white matter regions display unique characteristics in the first postnatal week of murine life. In both the corpus callosum and cerebellum microglia show amoeboid morphology and a similar transcription profile during development including high expression of genes related to priming of microglia, phagocytosis and migration at P7; characteristics which are already lost at P10. Together these data verify our previous transcriptional data obtained by microarray analysis and enable a more complete view into white matter and grey matter microglia at different developmental stages. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13104-019-4410-1) contains supplementary material, which is available to authorized users. BioMed Central 2019-07-01 /pmc/articles/PMC6604453/ /pubmed/31262353 http://dx.doi.org/10.1186/s13104-019-4410-1 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Note
Staszewski, Ori
Hagemeyer, Nora
Unique microglia expression profile in developing white matter
title Unique microglia expression profile in developing white matter
title_full Unique microglia expression profile in developing white matter
title_fullStr Unique microglia expression profile in developing white matter
title_full_unstemmed Unique microglia expression profile in developing white matter
title_short Unique microglia expression profile in developing white matter
title_sort unique microglia expression profile in developing white matter
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6604453/
https://www.ncbi.nlm.nih.gov/pubmed/31262353
http://dx.doi.org/10.1186/s13104-019-4410-1
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