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Unique microglia expression profile in developing white matter
OBJECTIVE: Recently we demonstrated that amoeboid microglia in white matter regions are essential for proper oligodendrocyte homeostasis and myelinogenesis in the first postnatal week. Amoeboid microglia in the mouse corpus callosum change their activation profile within few days after postnatal day...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6604453/ https://www.ncbi.nlm.nih.gov/pubmed/31262353 http://dx.doi.org/10.1186/s13104-019-4410-1 |
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author | Staszewski, Ori Hagemeyer, Nora |
author_facet | Staszewski, Ori Hagemeyer, Nora |
author_sort | Staszewski, Ori |
collection | PubMed |
description | OBJECTIVE: Recently we demonstrated that amoeboid microglia in white matter regions are essential for proper oligodendrocyte homeostasis and myelinogenesis in the first postnatal week. Amoeboid microglia in the mouse corpus callosum change their activation profile within few days after postnatal day (P)7 with microglia of the cerebellum showing similar features. Here we expanded our previous transcriptional analysis and performed detailed bulk RNA sequencing of microglia from corpus callosum, cortex and cerebellum at P7, P10 and P42. The goal of this study was to identify a specific gene profile for both, white matter and grey matter microglia during development. RESULTS: Microglia in white matter regions display unique characteristics in the first postnatal week of murine life. In both the corpus callosum and cerebellum microglia show amoeboid morphology and a similar transcription profile during development including high expression of genes related to priming of microglia, phagocytosis and migration at P7; characteristics which are already lost at P10. Together these data verify our previous transcriptional data obtained by microarray analysis and enable a more complete view into white matter and grey matter microglia at different developmental stages. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13104-019-4410-1) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6604453 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-66044532019-07-12 Unique microglia expression profile in developing white matter Staszewski, Ori Hagemeyer, Nora BMC Res Notes Research Note OBJECTIVE: Recently we demonstrated that amoeboid microglia in white matter regions are essential for proper oligodendrocyte homeostasis and myelinogenesis in the first postnatal week. Amoeboid microglia in the mouse corpus callosum change their activation profile within few days after postnatal day (P)7 with microglia of the cerebellum showing similar features. Here we expanded our previous transcriptional analysis and performed detailed bulk RNA sequencing of microglia from corpus callosum, cortex and cerebellum at P7, P10 and P42. The goal of this study was to identify a specific gene profile for both, white matter and grey matter microglia during development. RESULTS: Microglia in white matter regions display unique characteristics in the first postnatal week of murine life. In both the corpus callosum and cerebellum microglia show amoeboid morphology and a similar transcription profile during development including high expression of genes related to priming of microglia, phagocytosis and migration at P7; characteristics which are already lost at P10. Together these data verify our previous transcriptional data obtained by microarray analysis and enable a more complete view into white matter and grey matter microglia at different developmental stages. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13104-019-4410-1) contains supplementary material, which is available to authorized users. BioMed Central 2019-07-01 /pmc/articles/PMC6604453/ /pubmed/31262353 http://dx.doi.org/10.1186/s13104-019-4410-1 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Note Staszewski, Ori Hagemeyer, Nora Unique microglia expression profile in developing white matter |
title | Unique microglia expression profile in developing white matter |
title_full | Unique microglia expression profile in developing white matter |
title_fullStr | Unique microglia expression profile in developing white matter |
title_full_unstemmed | Unique microglia expression profile in developing white matter |
title_short | Unique microglia expression profile in developing white matter |
title_sort | unique microglia expression profile in developing white matter |
topic | Research Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6604453/ https://www.ncbi.nlm.nih.gov/pubmed/31262353 http://dx.doi.org/10.1186/s13104-019-4410-1 |
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