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Aflatoxin B(1)-Adsorbing Capability of Pleurotus eryngii Mycelium: Efficiency and Modeling of the Process

Aflatoxin B(1) (AfB(1)) is a carcinogenic mycotoxin that contaminates food and feed worldwide. We determined the AfB(1)-adsorption capability of non-viable Pleurotus eryngii mycelium, an edible fungus, as a potential means for removal of AfB(1) from contaminated solutions. Lyophilized mycelium was p...

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Autores principales: Haidukowski, Miriam, Casamassima, Eliana, Cimmarusti, Maria Teresa, Branà, Maria Teresa, Longobardi, Francesco, Acquafredda, Pasquale, Logrieco, Antonio, Altomare, Claudio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6604724/
https://www.ncbi.nlm.nih.gov/pubmed/31293538
http://dx.doi.org/10.3389/fmicb.2019.01386
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author Haidukowski, Miriam
Casamassima, Eliana
Cimmarusti, Maria Teresa
Branà, Maria Teresa
Longobardi, Francesco
Acquafredda, Pasquale
Logrieco, Antonio
Altomare, Claudio
author_facet Haidukowski, Miriam
Casamassima, Eliana
Cimmarusti, Maria Teresa
Branà, Maria Teresa
Longobardi, Francesco
Acquafredda, Pasquale
Logrieco, Antonio
Altomare, Claudio
author_sort Haidukowski, Miriam
collection PubMed
description Aflatoxin B(1) (AfB(1)) is a carcinogenic mycotoxin that contaminates food and feed worldwide. We determined the AfB(1)-adsorption capability of non-viable Pleurotus eryngii mycelium, an edible fungus, as a potential means for removal of AfB(1) from contaminated solutions. Lyophilized mycelium was produced and made enzymatically inert by sterilization at high temperatures. The material thus obtained was characterized by scanning electron microscopy with regard to the morpho-structural properties of the mycotoxin-adsorbing surfaces. The active surfaces appeared rough and sponge-like. The AfB(1)-mycelium system reached equilibrium at 37°C, 30 min, and pH 5–7, conditions that are compatible with the gastro-intestinal system of animals. The system remained stable for 48 h at room temperature, at pH 3, pH 7, and pH 7.4. A thermodynamic study of the process showed that this is a spontaneous and physical adsorption process, with a maximum of 85 ± 13% of removal efficiency of AfB(1) by P. eryngii mycelium. These results suggest that biosorbent materials obtained from the mycelium of the mushroom P. eryngii could be used as a low-cost and effective feed additive for AfB(1) detoxification.
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spelling pubmed-66047242019-07-10 Aflatoxin B(1)-Adsorbing Capability of Pleurotus eryngii Mycelium: Efficiency and Modeling of the Process Haidukowski, Miriam Casamassima, Eliana Cimmarusti, Maria Teresa Branà, Maria Teresa Longobardi, Francesco Acquafredda, Pasquale Logrieco, Antonio Altomare, Claudio Front Microbiol Microbiology Aflatoxin B(1) (AfB(1)) is a carcinogenic mycotoxin that contaminates food and feed worldwide. We determined the AfB(1)-adsorption capability of non-viable Pleurotus eryngii mycelium, an edible fungus, as a potential means for removal of AfB(1) from contaminated solutions. Lyophilized mycelium was produced and made enzymatically inert by sterilization at high temperatures. The material thus obtained was characterized by scanning electron microscopy with regard to the morpho-structural properties of the mycotoxin-adsorbing surfaces. The active surfaces appeared rough and sponge-like. The AfB(1)-mycelium system reached equilibrium at 37°C, 30 min, and pH 5–7, conditions that are compatible with the gastro-intestinal system of animals. The system remained stable for 48 h at room temperature, at pH 3, pH 7, and pH 7.4. A thermodynamic study of the process showed that this is a spontaneous and physical adsorption process, with a maximum of 85 ± 13% of removal efficiency of AfB(1) by P. eryngii mycelium. These results suggest that biosorbent materials obtained from the mycelium of the mushroom P. eryngii could be used as a low-cost and effective feed additive for AfB(1) detoxification. Frontiers Media S.A. 2019-06-25 /pmc/articles/PMC6604724/ /pubmed/31293538 http://dx.doi.org/10.3389/fmicb.2019.01386 Text en Copyright © 2019 Haidukowski, Casamassima, Cimmarusti, Branà, Longobardi, Acquafredda, Logrieco and Altomare. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Haidukowski, Miriam
Casamassima, Eliana
Cimmarusti, Maria Teresa
Branà, Maria Teresa
Longobardi, Francesco
Acquafredda, Pasquale
Logrieco, Antonio
Altomare, Claudio
Aflatoxin B(1)-Adsorbing Capability of Pleurotus eryngii Mycelium: Efficiency and Modeling of the Process
title Aflatoxin B(1)-Adsorbing Capability of Pleurotus eryngii Mycelium: Efficiency and Modeling of the Process
title_full Aflatoxin B(1)-Adsorbing Capability of Pleurotus eryngii Mycelium: Efficiency and Modeling of the Process
title_fullStr Aflatoxin B(1)-Adsorbing Capability of Pleurotus eryngii Mycelium: Efficiency and Modeling of the Process
title_full_unstemmed Aflatoxin B(1)-Adsorbing Capability of Pleurotus eryngii Mycelium: Efficiency and Modeling of the Process
title_short Aflatoxin B(1)-Adsorbing Capability of Pleurotus eryngii Mycelium: Efficiency and Modeling of the Process
title_sort aflatoxin b(1)-adsorbing capability of pleurotus eryngii mycelium: efficiency and modeling of the process
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6604724/
https://www.ncbi.nlm.nih.gov/pubmed/31293538
http://dx.doi.org/10.3389/fmicb.2019.01386
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