Cargando…
New and old tools to evaluate new antimicrobial peptides
The emergence of antimicrobial resistance due to the overuse of antimicrobials together with the existence of naturally untreatable infections well demonstrates the need for new instruments to fight microbes. Antimicrobial peptides (AMPs) are a promising family of molecules in this regard, because t...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AIMS Press
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6604946/ https://www.ncbi.nlm.nih.gov/pubmed/31294231 http://dx.doi.org/10.3934/microbiol.2018.3.522 |
_version_ | 1783431774012964864 |
---|---|
author | Rudilla, Hector Merlos, Alexandra Sans-Serramitjana, Eulàlia Fusté, Ester Sierra, Josep M. Zalacaín, Antonio Vinuesa, Teresa Viñas, Miguel |
author_facet | Rudilla, Hector Merlos, Alexandra Sans-Serramitjana, Eulàlia Fusté, Ester Sierra, Josep M. Zalacaín, Antonio Vinuesa, Teresa Viñas, Miguel |
author_sort | Rudilla, Hector |
collection | PubMed |
description | The emergence of antimicrobial resistance due to the overuse of antimicrobials together with the existence of naturally untreatable infections well demonstrates the need for new instruments to fight microbes. Antimicrobial peptides (AMPs) are a promising family of molecules in this regard, because they abundantly occur in nature and the results of preliminary studies of their clinical potential have been encouraging. However, further progress will benefit from the standardization of research methods to assess the antimicrobial properties of AMPs. Here we review the diverse methods used to study the antimicrobial power of AMPs and recommend a pathway to explore new molecules. The use of new methodologies to quantitatively evaluate the physical effect on bacterial biofilms such as force spectroscopy and surface cell damage evaluation, constitute novel approaches to study new AMPs. |
format | Online Article Text |
id | pubmed-6604946 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | AIMS Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-66049462019-07-10 New and old tools to evaluate new antimicrobial peptides Rudilla, Hector Merlos, Alexandra Sans-Serramitjana, Eulàlia Fusté, Ester Sierra, Josep M. Zalacaín, Antonio Vinuesa, Teresa Viñas, Miguel AIMS Microbiol Review The emergence of antimicrobial resistance due to the overuse of antimicrobials together with the existence of naturally untreatable infections well demonstrates the need for new instruments to fight microbes. Antimicrobial peptides (AMPs) are a promising family of molecules in this regard, because they abundantly occur in nature and the results of preliminary studies of their clinical potential have been encouraging. However, further progress will benefit from the standardization of research methods to assess the antimicrobial properties of AMPs. Here we review the diverse methods used to study the antimicrobial power of AMPs and recommend a pathway to explore new molecules. The use of new methodologies to quantitatively evaluate the physical effect on bacterial biofilms such as force spectroscopy and surface cell damage evaluation, constitute novel approaches to study new AMPs. AIMS Press 2018-06-29 /pmc/articles/PMC6604946/ /pubmed/31294231 http://dx.doi.org/10.3934/microbiol.2018.3.522 Text en © 2018 the Author(s), licensee AIMS Press This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) |
spellingShingle | Review Rudilla, Hector Merlos, Alexandra Sans-Serramitjana, Eulàlia Fusté, Ester Sierra, Josep M. Zalacaín, Antonio Vinuesa, Teresa Viñas, Miguel New and old tools to evaluate new antimicrobial peptides |
title | New and old tools to evaluate new antimicrobial peptides |
title_full | New and old tools to evaluate new antimicrobial peptides |
title_fullStr | New and old tools to evaluate new antimicrobial peptides |
title_full_unstemmed | New and old tools to evaluate new antimicrobial peptides |
title_short | New and old tools to evaluate new antimicrobial peptides |
title_sort | new and old tools to evaluate new antimicrobial peptides |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6604946/ https://www.ncbi.nlm.nih.gov/pubmed/31294231 http://dx.doi.org/10.3934/microbiol.2018.3.522 |
work_keys_str_mv | AT rudillahector newandoldtoolstoevaluatenewantimicrobialpeptides AT merlosalexandra newandoldtoolstoevaluatenewantimicrobialpeptides AT sansserramitjanaeulalia newandoldtoolstoevaluatenewantimicrobialpeptides AT fusteester newandoldtoolstoevaluatenewantimicrobialpeptides AT sierrajosepm newandoldtoolstoevaluatenewantimicrobialpeptides AT zalacainantonio newandoldtoolstoevaluatenewantimicrobialpeptides AT vinuesateresa newandoldtoolstoevaluatenewantimicrobialpeptides AT vinasmiguel newandoldtoolstoevaluatenewantimicrobialpeptides |