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Proliferative memory SAMHD1(low) CD4(+) T cells harbour high levels of HIV-1 with compartmentalized viral populations

We previously reported the presence of memory CD4(+) T cells that express low levels of SAMHD1 (SAMHD1(low)) in peripheral blood and lymph nodes from both HIV-1 infected and uninfected individuals. These cells are enriched in Th17 and Tfh subsets, two populations known to be preferentially targeted...

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Autores principales: Hani, Lylia, Chaillon, Antoine, Nere, Marie-Laure, Ruffin, Nicolas, Alameddine, Joudy, Salmona, Maud, Lopez Zaragoza, José-Luiz, Smith, Davey M., Schwartz, Olivier, Lelièvre, Jean-Daniel, Delaugerre, Constance, Lévy, Yves, Seddiki, Nabila
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6605680/
https://www.ncbi.nlm.nih.gov/pubmed/31220191
http://dx.doi.org/10.1371/journal.ppat.1007868
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author Hani, Lylia
Chaillon, Antoine
Nere, Marie-Laure
Ruffin, Nicolas
Alameddine, Joudy
Salmona, Maud
Lopez Zaragoza, José-Luiz
Smith, Davey M.
Schwartz, Olivier
Lelièvre, Jean-Daniel
Delaugerre, Constance
Lévy, Yves
Seddiki, Nabila
author_facet Hani, Lylia
Chaillon, Antoine
Nere, Marie-Laure
Ruffin, Nicolas
Alameddine, Joudy
Salmona, Maud
Lopez Zaragoza, José-Luiz
Smith, Davey M.
Schwartz, Olivier
Lelièvre, Jean-Daniel
Delaugerre, Constance
Lévy, Yves
Seddiki, Nabila
author_sort Hani, Lylia
collection PubMed
description We previously reported the presence of memory CD4(+) T cells that express low levels of SAMHD1 (SAMHD1(low)) in peripheral blood and lymph nodes from both HIV-1 infected and uninfected individuals. These cells are enriched in Th17 and Tfh subsets, two populations known to be preferentially targeted by HIV-1. Here we investigated whether SAMHD1(low) CD4(+) T-cells harbour replication-competent virus and compartimentalized HIV-1 genomes. We sorted memory CD4(+)CD45RO(+)SAMHD1(low), CD4(+)CD45RO(+)SAMHD1(+) and naive CD4(+)CD45RO(-)SAMHD1(+) cells from HIV-1-infected patients on anti-retroviral therapy (c-ART) and performed HIV-1 DNA quantification, ultra-deep-sequencing of partial env (C2/V3) sequences and phenotypic characterization of the cells. We show that SAMHD1(low) cells include novel Th17 CCR6(+) subsets that lack CXCR3 and CCR4 (CCR6(+)DN). There is a decrease of the % of Th17 in SAMHD1(low) compartment in infected compared to uninfected individuals (41% vs 55%, p<0.05), whereas the % of CCR6(+)DN increases (7.95% vs 3.8%, p<0.05). Moreover, in HIV-1 infected patients, memory SAMHD1(low) cells harbour high levels of HIV-1 DNA compared to memory SAMHD1(+) cells (4.5 vs 3.8 log/10(6)cells, respectively, p<0.001), while naïve SAMHD1(+) showed significantly lower levels (3.1 log/10(6)cells, p<0.0001). Importantly, we show that SAMHD1(low) cells contain p24-producing cells. Moreover, phylogenetic analyses revealed well-segregated HIV-1 DNA populations with compartmentalization between SAMHD1(low) and SAMHD1(+) memory cells, and limited viral exchange. As expected, the % of Ki67(+) cells was significantly higher in SAMHD1(low) compared to SAMHD1(+) cells. There was positive association between levels of HIV-1 DNA and Ki67(+) in memory SAMHD1(low) cells, but not in memory and naïve SAMHD1(+) CD4(+) T-cells. Altogether, these data suggest that proliferative memory SAMHD1(low) cells contribute to viral persistence.
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spelling pubmed-66056802019-07-12 Proliferative memory SAMHD1(low) CD4(+) T cells harbour high levels of HIV-1 with compartmentalized viral populations Hani, Lylia Chaillon, Antoine Nere, Marie-Laure Ruffin, Nicolas Alameddine, Joudy Salmona, Maud Lopez Zaragoza, José-Luiz Smith, Davey M. Schwartz, Olivier Lelièvre, Jean-Daniel Delaugerre, Constance Lévy, Yves Seddiki, Nabila PLoS Pathog Research Article We previously reported the presence of memory CD4(+) T cells that express low levels of SAMHD1 (SAMHD1(low)) in peripheral blood and lymph nodes from both HIV-1 infected and uninfected individuals. These cells are enriched in Th17 and Tfh subsets, two populations known to be preferentially targeted by HIV-1. Here we investigated whether SAMHD1(low) CD4(+) T-cells harbour replication-competent virus and compartimentalized HIV-1 genomes. We sorted memory CD4(+)CD45RO(+)SAMHD1(low), CD4(+)CD45RO(+)SAMHD1(+) and naive CD4(+)CD45RO(-)SAMHD1(+) cells from HIV-1-infected patients on anti-retroviral therapy (c-ART) and performed HIV-1 DNA quantification, ultra-deep-sequencing of partial env (C2/V3) sequences and phenotypic characterization of the cells. We show that SAMHD1(low) cells include novel Th17 CCR6(+) subsets that lack CXCR3 and CCR4 (CCR6(+)DN). There is a decrease of the % of Th17 in SAMHD1(low) compartment in infected compared to uninfected individuals (41% vs 55%, p<0.05), whereas the % of CCR6(+)DN increases (7.95% vs 3.8%, p<0.05). Moreover, in HIV-1 infected patients, memory SAMHD1(low) cells harbour high levels of HIV-1 DNA compared to memory SAMHD1(+) cells (4.5 vs 3.8 log/10(6)cells, respectively, p<0.001), while naïve SAMHD1(+) showed significantly lower levels (3.1 log/10(6)cells, p<0.0001). Importantly, we show that SAMHD1(low) cells contain p24-producing cells. Moreover, phylogenetic analyses revealed well-segregated HIV-1 DNA populations with compartmentalization between SAMHD1(low) and SAMHD1(+) memory cells, and limited viral exchange. As expected, the % of Ki67(+) cells was significantly higher in SAMHD1(low) compared to SAMHD1(+) cells. There was positive association between levels of HIV-1 DNA and Ki67(+) in memory SAMHD1(low) cells, but not in memory and naïve SAMHD1(+) CD4(+) T-cells. Altogether, these data suggest that proliferative memory SAMHD1(low) cells contribute to viral persistence. Public Library of Science 2019-06-20 /pmc/articles/PMC6605680/ /pubmed/31220191 http://dx.doi.org/10.1371/journal.ppat.1007868 Text en © 2019 Hani et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Hani, Lylia
Chaillon, Antoine
Nere, Marie-Laure
Ruffin, Nicolas
Alameddine, Joudy
Salmona, Maud
Lopez Zaragoza, José-Luiz
Smith, Davey M.
Schwartz, Olivier
Lelièvre, Jean-Daniel
Delaugerre, Constance
Lévy, Yves
Seddiki, Nabila
Proliferative memory SAMHD1(low) CD4(+) T cells harbour high levels of HIV-1 with compartmentalized viral populations
title Proliferative memory SAMHD1(low) CD4(+) T cells harbour high levels of HIV-1 with compartmentalized viral populations
title_full Proliferative memory SAMHD1(low) CD4(+) T cells harbour high levels of HIV-1 with compartmentalized viral populations
title_fullStr Proliferative memory SAMHD1(low) CD4(+) T cells harbour high levels of HIV-1 with compartmentalized viral populations
title_full_unstemmed Proliferative memory SAMHD1(low) CD4(+) T cells harbour high levels of HIV-1 with compartmentalized viral populations
title_short Proliferative memory SAMHD1(low) CD4(+) T cells harbour high levels of HIV-1 with compartmentalized viral populations
title_sort proliferative memory samhd1(low) cd4(+) t cells harbour high levels of hiv-1 with compartmentalized viral populations
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6605680/
https://www.ncbi.nlm.nih.gov/pubmed/31220191
http://dx.doi.org/10.1371/journal.ppat.1007868
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