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Hlf marks the developmental pathway for hematopoietic stem cells but not for erythro-myeloid progenitors
Before the emergence of hematopoietic stem cells (HSCs), lineage-restricted progenitors, such as erythro-myeloid progenitors (EMPs), are detected in the embryo or in pluripotent stem cell cultures in vitro. Although both HSCs and EMPs are derived from hemogenic endothelium, it remains unclear how an...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Rockefeller University Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6605751/ https://www.ncbi.nlm.nih.gov/pubmed/31076455 http://dx.doi.org/10.1084/jem.20181399 |
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author | Yokomizo, Tomomasa Watanabe, Naoki Umemoto, Terumasa Matsuo, Junichi Harai, Ryota Kihara, Yoshihiko Nakamura, Eri Tada, Norihiro Sato, Tomohiko Takaku, Tomoiku Shimono, Akihiko Takizawa, Hitoshi Nakagata, Naomi Mori, Seiichi Kurokawa, Mineo Tenen, Daniel G. Osato, Motomi Suda, Toshio Komatsu, Norio |
author_facet | Yokomizo, Tomomasa Watanabe, Naoki Umemoto, Terumasa Matsuo, Junichi Harai, Ryota Kihara, Yoshihiko Nakamura, Eri Tada, Norihiro Sato, Tomohiko Takaku, Tomoiku Shimono, Akihiko Takizawa, Hitoshi Nakagata, Naomi Mori, Seiichi Kurokawa, Mineo Tenen, Daniel G. Osato, Motomi Suda, Toshio Komatsu, Norio |
author_sort | Yokomizo, Tomomasa |
collection | PubMed |
description | Before the emergence of hematopoietic stem cells (HSCs), lineage-restricted progenitors, such as erythro-myeloid progenitors (EMPs), are detected in the embryo or in pluripotent stem cell cultures in vitro. Although both HSCs and EMPs are derived from hemogenic endothelium, it remains unclear how and when these two developmental programs are segregated during ontogeny. Here, we show that hepatic leukemia factor (Hlf) expression specifically marks a developmental continuum between HSC precursors and HSCs. Using the Hlf-tdTomato reporter mouse, we found that Hlf is expressed in intra-aortic hematopoietic clusters and fetal liver HSCs. In contrast, EMPs and yolk sac hematopoietic clusters before embryonic day 9.5 do not express Hlf. HSC specification, regulated by the Evi-1/Hlf axis, is activated only within Hlf(+) nascent hematopoietic clusters. These results strongly suggest that HSCs and EMPs are generated from distinct cohorts of hemogenic endothelium. Selective induction of the Hlf(+) lineage pathway may lead to the in vitro generation of HSCs from pluripotent stem cells. |
format | Online Article Text |
id | pubmed-6605751 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-66057512020-01-01 Hlf marks the developmental pathway for hematopoietic stem cells but not for erythro-myeloid progenitors Yokomizo, Tomomasa Watanabe, Naoki Umemoto, Terumasa Matsuo, Junichi Harai, Ryota Kihara, Yoshihiko Nakamura, Eri Tada, Norihiro Sato, Tomohiko Takaku, Tomoiku Shimono, Akihiko Takizawa, Hitoshi Nakagata, Naomi Mori, Seiichi Kurokawa, Mineo Tenen, Daniel G. Osato, Motomi Suda, Toshio Komatsu, Norio J Exp Med Research Articles Before the emergence of hematopoietic stem cells (HSCs), lineage-restricted progenitors, such as erythro-myeloid progenitors (EMPs), are detected in the embryo or in pluripotent stem cell cultures in vitro. Although both HSCs and EMPs are derived from hemogenic endothelium, it remains unclear how and when these two developmental programs are segregated during ontogeny. Here, we show that hepatic leukemia factor (Hlf) expression specifically marks a developmental continuum between HSC precursors and HSCs. Using the Hlf-tdTomato reporter mouse, we found that Hlf is expressed in intra-aortic hematopoietic clusters and fetal liver HSCs. In contrast, EMPs and yolk sac hematopoietic clusters before embryonic day 9.5 do not express Hlf. HSC specification, regulated by the Evi-1/Hlf axis, is activated only within Hlf(+) nascent hematopoietic clusters. These results strongly suggest that HSCs and EMPs are generated from distinct cohorts of hemogenic endothelium. Selective induction of the Hlf(+) lineage pathway may lead to the in vitro generation of HSCs from pluripotent stem cells. Rockefeller University Press 2019-07-01 2019-05-10 /pmc/articles/PMC6605751/ /pubmed/31076455 http://dx.doi.org/10.1084/jem.20181399 Text en © 2019 Yokomizo et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Yokomizo, Tomomasa Watanabe, Naoki Umemoto, Terumasa Matsuo, Junichi Harai, Ryota Kihara, Yoshihiko Nakamura, Eri Tada, Norihiro Sato, Tomohiko Takaku, Tomoiku Shimono, Akihiko Takizawa, Hitoshi Nakagata, Naomi Mori, Seiichi Kurokawa, Mineo Tenen, Daniel G. Osato, Motomi Suda, Toshio Komatsu, Norio Hlf marks the developmental pathway for hematopoietic stem cells but not for erythro-myeloid progenitors |
title | Hlf marks the developmental pathway for hematopoietic stem cells but not for erythro-myeloid progenitors |
title_full | Hlf marks the developmental pathway for hematopoietic stem cells but not for erythro-myeloid progenitors |
title_fullStr | Hlf marks the developmental pathway for hematopoietic stem cells but not for erythro-myeloid progenitors |
title_full_unstemmed | Hlf marks the developmental pathway for hematopoietic stem cells but not for erythro-myeloid progenitors |
title_short | Hlf marks the developmental pathway for hematopoietic stem cells but not for erythro-myeloid progenitors |
title_sort | hlf marks the developmental pathway for hematopoietic stem cells but not for erythro-myeloid progenitors |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6605751/ https://www.ncbi.nlm.nih.gov/pubmed/31076455 http://dx.doi.org/10.1084/jem.20181399 |
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