Carotenoids from UV-resistant Antarctic Microbacterium sp. LEMMJ01

The Microbacterium sp. LEMMJ01 isolated from Antarctic soil does not belong to any of the nearest species identified in the RDP database. Under UV radiation (A, B and C wavebands) the survival fractions of Microbacterium sp. cells were much higher compared with wild-type E. coli K12A15. Especially r...

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Autores principales: Reis-Mansur, Maria Cristina P. P., Cardoso-Rurr, Janine S., Silva, Josemar V. Maiworm Abreu, de Souza, Gabriela Rodrigues, Cardoso, Verônica da Silva, Mansoldo, Felipe Raposo Passos, Pinheiro, Yuri, Schultz, Júnia, Lopez Balottin, Luciene B., da Silva, Antonio Jorge Ribeiro, Lage, Claudia, dos Santos, Elisabete Pereira, Rosado, Alexandre Soares, Vermelho, Alane Beatriz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6606617/
https://www.ncbi.nlm.nih.gov/pubmed/31266976
http://dx.doi.org/10.1038/s41598-019-45840-6
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author Reis-Mansur, Maria Cristina P. P.
Cardoso-Rurr, Janine S.
Silva, Josemar V. Maiworm Abreu
de Souza, Gabriela Rodrigues
Cardoso, Verônica da Silva
Mansoldo, Felipe Raposo Passos
Pinheiro, Yuri
Schultz, Júnia
Lopez Balottin, Luciene B.
da Silva, Antonio Jorge Ribeiro
Lage, Claudia
dos Santos, Elisabete Pereira
Rosado, Alexandre Soares
Vermelho, Alane Beatriz
author_facet Reis-Mansur, Maria Cristina P. P.
Cardoso-Rurr, Janine S.
Silva, Josemar V. Maiworm Abreu
de Souza, Gabriela Rodrigues
Cardoso, Verônica da Silva
Mansoldo, Felipe Raposo Passos
Pinheiro, Yuri
Schultz, Júnia
Lopez Balottin, Luciene B.
da Silva, Antonio Jorge Ribeiro
Lage, Claudia
dos Santos, Elisabete Pereira
Rosado, Alexandre Soares
Vermelho, Alane Beatriz
author_sort Reis-Mansur, Maria Cristina P. P.
collection PubMed
description The Microbacterium sp. LEMMJ01 isolated from Antarctic soil does not belong to any of the nearest species identified in the RDP database. Under UV radiation (A, B and C wavebands) the survival fractions of Microbacterium sp. cells were much higher compared with wild-type E. coli K12A15. Especially remarkable for an Antarctic bacterium, an expressive resistance against high UV-B doses was observed. The increased survival of DNA repair-proficient E. coli grown overnight added of 0.1 mg/ml or 1 mg/ml of the whole pigment extract produced by Microbacterium sp. revealed that part of the resistance of Microbacterium sp. against UV-B radiation seems to be connected with photoprotection by its pigments. Scanning electron microscopy revealed that UV-A and UV-B ensued membrane alterations only in E. coli. The APCI-MS fingerprints revealed the diagnostic ions for neurosporene (m/z 580, 566, 522, 538, and 524) synergism for the first time in this bacterium by HPLC-MS/MS analysis. Carotenoids also were devoid of phototoxicity and cytotoxicity effects in mouse cells and in human keratinocytes and fibroblasts.
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spelling pubmed-66066172019-07-14 Carotenoids from UV-resistant Antarctic Microbacterium sp. LEMMJ01 Reis-Mansur, Maria Cristina P. P. Cardoso-Rurr, Janine S. Silva, Josemar V. Maiworm Abreu de Souza, Gabriela Rodrigues Cardoso, Verônica da Silva Mansoldo, Felipe Raposo Passos Pinheiro, Yuri Schultz, Júnia Lopez Balottin, Luciene B. da Silva, Antonio Jorge Ribeiro Lage, Claudia dos Santos, Elisabete Pereira Rosado, Alexandre Soares Vermelho, Alane Beatriz Sci Rep Article The Microbacterium sp. LEMMJ01 isolated from Antarctic soil does not belong to any of the nearest species identified in the RDP database. Under UV radiation (A, B and C wavebands) the survival fractions of Microbacterium sp. cells were much higher compared with wild-type E. coli K12A15. Especially remarkable for an Antarctic bacterium, an expressive resistance against high UV-B doses was observed. The increased survival of DNA repair-proficient E. coli grown overnight added of 0.1 mg/ml or 1 mg/ml of the whole pigment extract produced by Microbacterium sp. revealed that part of the resistance of Microbacterium sp. against UV-B radiation seems to be connected with photoprotection by its pigments. Scanning electron microscopy revealed that UV-A and UV-B ensued membrane alterations only in E. coli. The APCI-MS fingerprints revealed the diagnostic ions for neurosporene (m/z 580, 566, 522, 538, and 524) synergism for the first time in this bacterium by HPLC-MS/MS analysis. Carotenoids also were devoid of phototoxicity and cytotoxicity effects in mouse cells and in human keratinocytes and fibroblasts. Nature Publishing Group UK 2019-07-02 /pmc/articles/PMC6606617/ /pubmed/31266976 http://dx.doi.org/10.1038/s41598-019-45840-6 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Reis-Mansur, Maria Cristina P. P.
Cardoso-Rurr, Janine S.
Silva, Josemar V. Maiworm Abreu
de Souza, Gabriela Rodrigues
Cardoso, Verônica da Silva
Mansoldo, Felipe Raposo Passos
Pinheiro, Yuri
Schultz, Júnia
Lopez Balottin, Luciene B.
da Silva, Antonio Jorge Ribeiro
Lage, Claudia
dos Santos, Elisabete Pereira
Rosado, Alexandre Soares
Vermelho, Alane Beatriz
Carotenoids from UV-resistant Antarctic Microbacterium sp. LEMMJ01
title Carotenoids from UV-resistant Antarctic Microbacterium sp. LEMMJ01
title_full Carotenoids from UV-resistant Antarctic Microbacterium sp. LEMMJ01
title_fullStr Carotenoids from UV-resistant Antarctic Microbacterium sp. LEMMJ01
title_full_unstemmed Carotenoids from UV-resistant Antarctic Microbacterium sp. LEMMJ01
title_short Carotenoids from UV-resistant Antarctic Microbacterium sp. LEMMJ01
title_sort carotenoids from uv-resistant antarctic microbacterium sp. lemmj01
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6606617/
https://www.ncbi.nlm.nih.gov/pubmed/31266976
http://dx.doi.org/10.1038/s41598-019-45840-6
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