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Effect of Ultrasound Combined With Microbubble Therapy on Interstitial Fluid Pressure and VX2 Tumor Structure in Rabbit

Interstitial fluid pressure (IFP) in tumor tissue is significantly higher than that in normal tissue, which reduces the effectiveness of therapeutic drugs. There are several methods to decrease the IFP, such as normalizing blood vessel, decreasing hyaluronic acid and collagen fiber content in the ex...

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Autores principales: Zhang, Qianyun, Jin, Hai, Chen, Liping, Chen, Qiaoli, He, Yan, Yang, Yuwen, Ma, Suihong, Xiao, Shuyi, Xi, Fen, Luo, Qiong, Liu, Jianhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6606793/
https://www.ncbi.nlm.nih.gov/pubmed/31293427
http://dx.doi.org/10.3389/fphar.2019.00716
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author Zhang, Qianyun
Jin, Hai
Chen, Liping
Chen, Qiaoli
He, Yan
Yang, Yuwen
Ma, Suihong
Xiao, Shuyi
Xi, Fen
Luo, Qiong
Liu, Jianhua
author_facet Zhang, Qianyun
Jin, Hai
Chen, Liping
Chen, Qiaoli
He, Yan
Yang, Yuwen
Ma, Suihong
Xiao, Shuyi
Xi, Fen
Luo, Qiong
Liu, Jianhua
author_sort Zhang, Qianyun
collection PubMed
description Interstitial fluid pressure (IFP) in tumor tissue is significantly higher than that in normal tissue, which reduces the effectiveness of therapeutic drugs. There are several methods to decrease the IFP, such as normalizing blood vessel, decreasing hyaluronic acid and collagen fiber content in the extracellular matrix (ECM), and recovering lymphatic function. Reducing tumor IFP might be developed as a novel approach in cancer therapy. In this study, we aimed to elucidate the relationship between ultrasound combined with microbubble therapy and IFP, and the associated mechanism. VX2 tumor in rabbit was treated with ultrasound combined with microbubbles at different intensities. The IFP was measured using the wick-in-needle (WIN) method. The collagen and reticular fibers were stained by Masson and Gordon–Sweets, respectively. The results showed that low-frequency non-focus ultrasound combined with microbubbles therapy influences the IFP in tumor tissues; low-frequency non-focus ultrasound with low pressure increased the IFP, whereas middle–high pressure decreased the IFP. The results showed that the structure and content of collagen and reticular fibers in tumor tissue were rarely influenced by the treatment. Our study provides a novel approach of reduced IFP antitumor therapy.
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spelling pubmed-66067932019-07-10 Effect of Ultrasound Combined With Microbubble Therapy on Interstitial Fluid Pressure and VX2 Tumor Structure in Rabbit Zhang, Qianyun Jin, Hai Chen, Liping Chen, Qiaoli He, Yan Yang, Yuwen Ma, Suihong Xiao, Shuyi Xi, Fen Luo, Qiong Liu, Jianhua Front Pharmacol Pharmacology Interstitial fluid pressure (IFP) in tumor tissue is significantly higher than that in normal tissue, which reduces the effectiveness of therapeutic drugs. There are several methods to decrease the IFP, such as normalizing blood vessel, decreasing hyaluronic acid and collagen fiber content in the extracellular matrix (ECM), and recovering lymphatic function. Reducing tumor IFP might be developed as a novel approach in cancer therapy. In this study, we aimed to elucidate the relationship between ultrasound combined with microbubble therapy and IFP, and the associated mechanism. VX2 tumor in rabbit was treated with ultrasound combined with microbubbles at different intensities. The IFP was measured using the wick-in-needle (WIN) method. The collagen and reticular fibers were stained by Masson and Gordon–Sweets, respectively. The results showed that low-frequency non-focus ultrasound combined with microbubbles therapy influences the IFP in tumor tissues; low-frequency non-focus ultrasound with low pressure increased the IFP, whereas middle–high pressure decreased the IFP. The results showed that the structure and content of collagen and reticular fibers in tumor tissue were rarely influenced by the treatment. Our study provides a novel approach of reduced IFP antitumor therapy. Frontiers Media S.A. 2019-06-26 /pmc/articles/PMC6606793/ /pubmed/31293427 http://dx.doi.org/10.3389/fphar.2019.00716 Text en Copyright © 2019 Zhang, Jin, Chen, Chen, He, Yang, Ma, Xiao, Xi, Luo and Liu http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Zhang, Qianyun
Jin, Hai
Chen, Liping
Chen, Qiaoli
He, Yan
Yang, Yuwen
Ma, Suihong
Xiao, Shuyi
Xi, Fen
Luo, Qiong
Liu, Jianhua
Effect of Ultrasound Combined With Microbubble Therapy on Interstitial Fluid Pressure and VX2 Tumor Structure in Rabbit
title Effect of Ultrasound Combined With Microbubble Therapy on Interstitial Fluid Pressure and VX2 Tumor Structure in Rabbit
title_full Effect of Ultrasound Combined With Microbubble Therapy on Interstitial Fluid Pressure and VX2 Tumor Structure in Rabbit
title_fullStr Effect of Ultrasound Combined With Microbubble Therapy on Interstitial Fluid Pressure and VX2 Tumor Structure in Rabbit
title_full_unstemmed Effect of Ultrasound Combined With Microbubble Therapy on Interstitial Fluid Pressure and VX2 Tumor Structure in Rabbit
title_short Effect of Ultrasound Combined With Microbubble Therapy on Interstitial Fluid Pressure and VX2 Tumor Structure in Rabbit
title_sort effect of ultrasound combined with microbubble therapy on interstitial fluid pressure and vx2 tumor structure in rabbit
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6606793/
https://www.ncbi.nlm.nih.gov/pubmed/31293427
http://dx.doi.org/10.3389/fphar.2019.00716
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