Cargando…

Fecal Short-Chain Fatty Acids Are Not Predictive of Colonic Tumor Status and Cannot Be Predicted Based on Bacterial Community Structure

Colonic bacterial populations are thought to have a role in the development of colorectal cancer with some protecting against inflammation and others exacerbating inflammation. Short-chain fatty acids (SCFAs) have been shown to have anti-inflammatory properties and are produced in large quantities b...

Descripción completa

Detalles Bibliográficos
Autores principales: Sze, Marc A., Topçuoğlu, Begüm D., Lesniak, Nicholas A., Ruffin, Mack T., Schloss, Patrick D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6606814/
https://www.ncbi.nlm.nih.gov/pubmed/31266879
http://dx.doi.org/10.1128/mBio.01454-19
_version_ 1783431975363674112
author Sze, Marc A.
Topçuoğlu, Begüm D.
Lesniak, Nicholas A.
Ruffin, Mack T.
Schloss, Patrick D.
author_facet Sze, Marc A.
Topçuoğlu, Begüm D.
Lesniak, Nicholas A.
Ruffin, Mack T.
Schloss, Patrick D.
author_sort Sze, Marc A.
collection PubMed
description Colonic bacterial populations are thought to have a role in the development of colorectal cancer with some protecting against inflammation and others exacerbating inflammation. Short-chain fatty acids (SCFAs) have been shown to have anti-inflammatory properties and are produced in large quantities by colonic bacteria that produce SCFAs by fermenting fiber. We assessed whether there was an association between fecal SCFA concentrations and the presence of colonic adenomas or carcinomas in a cohort of individuals using 16S rRNA gene and metagenomic shotgun sequence data. We measured the fecal concentrations of acetate, propionate, and butyrate within the cohort and found that there were no significant associations between SCFA concentration and tumor status. When we incorporated these concentrations into random forest classification models trained to differentiate between people with healthy colons and those with adenomas or carcinomas, we found that they did not significantly improve the ability of 16S rRNA gene or metagenomic gene sequence-based models to classify individuals. Finally, we generated random forest regression models trained to predict the concentration of each SCFA based on 16S rRNA gene or metagenomic gene sequence data from the same samples. These models performed poorly and were able to explain at most 14% of the observed variation in the SCFA concentrations. These results support the broader epidemiological data that questions the value of fiber consumption for reducing the risks of colorectal cancer. Although other bacterial metabolites may serve as biomarkers to detect adenomas or carcinomas, fecal SCFA concentrations have limited predictive power.
format Online
Article
Text
id pubmed-6606814
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher American Society for Microbiology
record_format MEDLINE/PubMed
spelling pubmed-66068142019-07-08 Fecal Short-Chain Fatty Acids Are Not Predictive of Colonic Tumor Status and Cannot Be Predicted Based on Bacterial Community Structure Sze, Marc A. Topçuoğlu, Begüm D. Lesniak, Nicholas A. Ruffin, Mack T. Schloss, Patrick D. mBio Observation Colonic bacterial populations are thought to have a role in the development of colorectal cancer with some protecting against inflammation and others exacerbating inflammation. Short-chain fatty acids (SCFAs) have been shown to have anti-inflammatory properties and are produced in large quantities by colonic bacteria that produce SCFAs by fermenting fiber. We assessed whether there was an association between fecal SCFA concentrations and the presence of colonic adenomas or carcinomas in a cohort of individuals using 16S rRNA gene and metagenomic shotgun sequence data. We measured the fecal concentrations of acetate, propionate, and butyrate within the cohort and found that there were no significant associations between SCFA concentration and tumor status. When we incorporated these concentrations into random forest classification models trained to differentiate between people with healthy colons and those with adenomas or carcinomas, we found that they did not significantly improve the ability of 16S rRNA gene or metagenomic gene sequence-based models to classify individuals. Finally, we generated random forest regression models trained to predict the concentration of each SCFA based on 16S rRNA gene or metagenomic gene sequence data from the same samples. These models performed poorly and were able to explain at most 14% of the observed variation in the SCFA concentrations. These results support the broader epidemiological data that questions the value of fiber consumption for reducing the risks of colorectal cancer. Although other bacterial metabolites may serve as biomarkers to detect adenomas or carcinomas, fecal SCFA concentrations have limited predictive power. American Society for Microbiology 2019-07-02 /pmc/articles/PMC6606814/ /pubmed/31266879 http://dx.doi.org/10.1128/mBio.01454-19 Text en Copyright © 2019 Sze et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Observation
Sze, Marc A.
Topçuoğlu, Begüm D.
Lesniak, Nicholas A.
Ruffin, Mack T.
Schloss, Patrick D.
Fecal Short-Chain Fatty Acids Are Not Predictive of Colonic Tumor Status and Cannot Be Predicted Based on Bacterial Community Structure
title Fecal Short-Chain Fatty Acids Are Not Predictive of Colonic Tumor Status and Cannot Be Predicted Based on Bacterial Community Structure
title_full Fecal Short-Chain Fatty Acids Are Not Predictive of Colonic Tumor Status and Cannot Be Predicted Based on Bacterial Community Structure
title_fullStr Fecal Short-Chain Fatty Acids Are Not Predictive of Colonic Tumor Status and Cannot Be Predicted Based on Bacterial Community Structure
title_full_unstemmed Fecal Short-Chain Fatty Acids Are Not Predictive of Colonic Tumor Status and Cannot Be Predicted Based on Bacterial Community Structure
title_short Fecal Short-Chain Fatty Acids Are Not Predictive of Colonic Tumor Status and Cannot Be Predicted Based on Bacterial Community Structure
title_sort fecal short-chain fatty acids are not predictive of colonic tumor status and cannot be predicted based on bacterial community structure
topic Observation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6606814/
https://www.ncbi.nlm.nih.gov/pubmed/31266879
http://dx.doi.org/10.1128/mBio.01454-19
work_keys_str_mv AT szemarca fecalshortchainfattyacidsarenotpredictiveofcolonictumorstatusandcannotbepredictedbasedonbacterialcommunitystructure
AT topcuoglubegumd fecalshortchainfattyacidsarenotpredictiveofcolonictumorstatusandcannotbepredictedbasedonbacterialcommunitystructure
AT lesniaknicholasa fecalshortchainfattyacidsarenotpredictiveofcolonictumorstatusandcannotbepredictedbasedonbacterialcommunitystructure
AT ruffinmackt fecalshortchainfattyacidsarenotpredictiveofcolonictumorstatusandcannotbepredictedbasedonbacterialcommunitystructure
AT schlosspatrickd fecalshortchainfattyacidsarenotpredictiveofcolonictumorstatusandcannotbepredictedbasedonbacterialcommunitystructure