Collection of Recombinant Rotaviruses Expressing Fluorescent Reporter Proteins

A collection of recombinant rotaviruses that express the fluorescent markers UnaG, mKate, mRuby, TagBFP, CFP, or YFP as separate proteins was generated. Genes for the fluorescent proteins were inserted into genome segment 7 without compromising expression of the protein NSP3. These recombinant rotav...

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Detalles Bibliográficos
Autores principales: Philip, Asha A., Herrin, Brittany E., Garcia, Maximiliano L., Abad, Andrew T., Katen, Sarah P., Patton, John T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2019
Materias:
Culture Collections/Mutant Libraries
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6606910/
https://www.ncbi.nlm.nih.gov/pubmed/31270196
http://dx.doi.org/10.1128/MRA.00523-19
Descripción
Sumario:A collection of recombinant rotaviruses that express the fluorescent markers UnaG, mKate, mRuby, TagBFP, CFP, or YFP as separate proteins was generated. Genes for the fluorescent proteins were inserted into genome segment 7 without compromising expression of the protein NSP3. These recombinant rotaviruses are valuable for analyzing rotavirus biology by fluorescence-based live-cell imaging.

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