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Synergistic effects of extracorporeal shockwave therapy and modified Ojayeonjonghwan on erectile dysfunction in an animal model of diabetes

PURPOSE: We investigated the synergy effect of extracorporeal shock wave therapy (ESWT) with modified Ojayeonjonghwan (Korean herbal formula, KH-204) in an animal model of diabetes mellitus (DM)-induced erectile dysfunction (ED). MATERIALS AND METHODS: Eight-week-old male Sprague-Dawley rats were us...

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Autores principales: Jeong, Hyun Cheol, Bae, Woong Jin, Zhu, Guan Qun, Jeon, Seung Hwan, Choi, Sae Woong, Kim, Su Jin, Cho, Hyuk Jin, Hong, Sung-Hoo, Lee, Ji Youl, Hwang, Sung Yeoun, Kim, Sae Woong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Urological Association 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6607077/
https://www.ncbi.nlm.nih.gov/pubmed/31294138
http://dx.doi.org/10.4111/icu.2019.60.4.285
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author Jeong, Hyun Cheol
Bae, Woong Jin
Zhu, Guan Qun
Jeon, Seung Hwan
Choi, Sae Woong
Kim, Su Jin
Cho, Hyuk Jin
Hong, Sung-Hoo
Lee, Ji Youl
Hwang, Sung Yeoun
Kim, Sae Woong
author_facet Jeong, Hyun Cheol
Bae, Woong Jin
Zhu, Guan Qun
Jeon, Seung Hwan
Choi, Sae Woong
Kim, Su Jin
Cho, Hyuk Jin
Hong, Sung-Hoo
Lee, Ji Youl
Hwang, Sung Yeoun
Kim, Sae Woong
author_sort Jeong, Hyun Cheol
collection PubMed
description PURPOSE: We investigated the synergy effect of extracorporeal shock wave therapy (ESWT) with modified Ojayeonjonghwan (Korean herbal formula, KH-204) in an animal model of diabetes mellitus (DM)-induced erectile dysfunction (ED). MATERIALS AND METHODS: Eight-week-old male Sprague-Dawley rats were used in this study. DM was induced by an intraperitoneal injection of streptozotocin. DM rats were divided into 5 groups (n=10 per group): group 1, control; group 2, DM; group 3, DM+ESWT; group 4, DM+KH-204; and group 5, DM+ESWT+KH-204. In ESWT groups, rats were treated with ESWT at the penis 3 times a week for 2 weeks under anesthesia. The KH-204 groups were treated with a daily oral dose of KH-204 for 12 weeks. After all treatments, intracavernosal pressure (ICP) was measured, and the cavernous tissues were evaluated by Masson's trichrome, immunohistochemistry and western blot analysis. RESULTS: ICP was evaluated as a measurement of erectile function. The DM+ESWT, DM+KH-204, and DM+ESWT+KH-204 groups showed significantly restored erectile function compared with the DM group (p<0.05). Among these groups, the DM+ESWT+KH-204 group showed the highest ICP. Moreover, ESWT and KH-204 treatment restored smooth muscle contents and many parameters related to potency (vascular endothelial growth factor, neuronal nitric oxide synthase (NOS), endothelial [NOS] and platelet endothelial cell adhesion molecule-1) compared with the DM group (p<0.05). CONCLUSIONS: We confirmed the potential efficacy of ESWT and KH-204 in the treatment of ED patients using an animal model. The combination treatment of KH-204 and ESWT is expected to have good potential clinical results in the future treatment of refractory ED.
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spelling pubmed-66070772019-07-10 Synergistic effects of extracorporeal shockwave therapy and modified Ojayeonjonghwan on erectile dysfunction in an animal model of diabetes Jeong, Hyun Cheol Bae, Woong Jin Zhu, Guan Qun Jeon, Seung Hwan Choi, Sae Woong Kim, Su Jin Cho, Hyuk Jin Hong, Sung-Hoo Lee, Ji Youl Hwang, Sung Yeoun Kim, Sae Woong Investig Clin Urol Original Article PURPOSE: We investigated the synergy effect of extracorporeal shock wave therapy (ESWT) with modified Ojayeonjonghwan (Korean herbal formula, KH-204) in an animal model of diabetes mellitus (DM)-induced erectile dysfunction (ED). MATERIALS AND METHODS: Eight-week-old male Sprague-Dawley rats were used in this study. DM was induced by an intraperitoneal injection of streptozotocin. DM rats were divided into 5 groups (n=10 per group): group 1, control; group 2, DM; group 3, DM+ESWT; group 4, DM+KH-204; and group 5, DM+ESWT+KH-204. In ESWT groups, rats were treated with ESWT at the penis 3 times a week for 2 weeks under anesthesia. The KH-204 groups were treated with a daily oral dose of KH-204 for 12 weeks. After all treatments, intracavernosal pressure (ICP) was measured, and the cavernous tissues were evaluated by Masson's trichrome, immunohistochemistry and western blot analysis. RESULTS: ICP was evaluated as a measurement of erectile function. The DM+ESWT, DM+KH-204, and DM+ESWT+KH-204 groups showed significantly restored erectile function compared with the DM group (p<0.05). Among these groups, the DM+ESWT+KH-204 group showed the highest ICP. Moreover, ESWT and KH-204 treatment restored smooth muscle contents and many parameters related to potency (vascular endothelial growth factor, neuronal nitric oxide synthase (NOS), endothelial [NOS] and platelet endothelial cell adhesion molecule-1) compared with the DM group (p<0.05). CONCLUSIONS: We confirmed the potential efficacy of ESWT and KH-204 in the treatment of ED patients using an animal model. The combination treatment of KH-204 and ESWT is expected to have good potential clinical results in the future treatment of refractory ED. The Korean Urological Association 2019-07 2019-06-05 /pmc/articles/PMC6607077/ /pubmed/31294138 http://dx.doi.org/10.4111/icu.2019.60.4.285 Text en © The Korean Urological Association, 2019 http://creativecommons.org/licenses/by-nc/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Jeong, Hyun Cheol
Bae, Woong Jin
Zhu, Guan Qun
Jeon, Seung Hwan
Choi, Sae Woong
Kim, Su Jin
Cho, Hyuk Jin
Hong, Sung-Hoo
Lee, Ji Youl
Hwang, Sung Yeoun
Kim, Sae Woong
Synergistic effects of extracorporeal shockwave therapy and modified Ojayeonjonghwan on erectile dysfunction in an animal model of diabetes
title Synergistic effects of extracorporeal shockwave therapy and modified Ojayeonjonghwan on erectile dysfunction in an animal model of diabetes
title_full Synergistic effects of extracorporeal shockwave therapy and modified Ojayeonjonghwan on erectile dysfunction in an animal model of diabetes
title_fullStr Synergistic effects of extracorporeal shockwave therapy and modified Ojayeonjonghwan on erectile dysfunction in an animal model of diabetes
title_full_unstemmed Synergistic effects of extracorporeal shockwave therapy and modified Ojayeonjonghwan on erectile dysfunction in an animal model of diabetes
title_short Synergistic effects of extracorporeal shockwave therapy and modified Ojayeonjonghwan on erectile dysfunction in an animal model of diabetes
title_sort synergistic effects of extracorporeal shockwave therapy and modified ojayeonjonghwan on erectile dysfunction in an animal model of diabetes
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6607077/
https://www.ncbi.nlm.nih.gov/pubmed/31294138
http://dx.doi.org/10.4111/icu.2019.60.4.285
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