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Evaluation of interleukin 8 and interleukin 10 cytokines in liquid based cervical cytology samples

INTRODUCTION: inflammatory cytokines have been associated with various cancers, including cervical cancers. Interpreting cytokine expression in liquid based cervical samples is quite challenging. This study is aimed at evaluating the levels of interleukin 8 and 10 in liquid based cervical samples. M...

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Autores principales: Osiagwu, Daniel David, Azenabor, Alfred Ederialo, Osijirin, Abimbola Akinduro, Awopetu, Philomina Ikuoyah, Oyegbami, Folasade Ruth
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The African Field Epidemiology Network 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6607241/
https://www.ncbi.nlm.nih.gov/pubmed/31303919
http://dx.doi.org/10.11604/pamj.2019.32.148.16314
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author Osiagwu, Daniel David
Azenabor, Alfred Ederialo
Osijirin, Abimbola Akinduro
Awopetu, Philomina Ikuoyah
Oyegbami, Folasade Ruth
author_facet Osiagwu, Daniel David
Azenabor, Alfred Ederialo
Osijirin, Abimbola Akinduro
Awopetu, Philomina Ikuoyah
Oyegbami, Folasade Ruth
author_sort Osiagwu, Daniel David
collection PubMed
description INTRODUCTION: inflammatory cytokines have been associated with various cancers, including cervical cancers. Interpreting cytokine expression in liquid based cervical samples is quite challenging. This study is aimed at evaluating the levels of interleukin 8 and 10 in liquid based cervical samples. METHODS: this is a descriptive analytical study carried out on eighty five (85) subjects aged between 23 and 68 years. Cervical samples were collected in liquid based medium and smears later examined after staining with Papanicolaou technique. These were categorized into low grade intra-epithelial lesion/malignancy, high grade intraepithelial lesion/malignancy according to the degree of dyskaryosis. Concentrations of interleukin 8 and interleukin 10 in the samples were determined by enzyme linked immunosorbent assay. RESULTS: the mean age, standard deviation (SD) of the study subjects were 40.6 (7.8) years. A total number of 79 females (92.9%) were negative for intra-epithelial lesion/malignancy (NILM), while 4 (4.71%) and 2 (2.35%) were positive for low grade intra-epithelial lesion/malignancy (LILM) and high grade intra-epithelial lesion (HILM) respectively. While mean levels of interleukin 8 increased with the degree of malignancy, (107.27 ± 11.88pg/ml) in LILM, (114.80 ± 2.12pg/ml) in HILM when compared with NILM (88.39 ± 18.06pg/ml), (f = 0.700, p = 0.018); the mean levels of interleukin 10 was comparable between these groups (p ≥ 0.05). Pearson correlation coefficient analysis showed a negative association between interleukin 8 and interleukin 10 (r = -1.999, p = 0.000) in LILM. CONCLUSION: interleukin 8 cytokines in cervical cancer is associated with the degree of malignancy. Possible anti-inflammatory effect of interleukin 10 was not observed.
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spelling pubmed-66072412019-07-12 Evaluation of interleukin 8 and interleukin 10 cytokines in liquid based cervical cytology samples Osiagwu, Daniel David Azenabor, Alfred Ederialo Osijirin, Abimbola Akinduro Awopetu, Philomina Ikuoyah Oyegbami, Folasade Ruth Pan Afr Med J Research INTRODUCTION: inflammatory cytokines have been associated with various cancers, including cervical cancers. Interpreting cytokine expression in liquid based cervical samples is quite challenging. This study is aimed at evaluating the levels of interleukin 8 and 10 in liquid based cervical samples. METHODS: this is a descriptive analytical study carried out on eighty five (85) subjects aged between 23 and 68 years. Cervical samples were collected in liquid based medium and smears later examined after staining with Papanicolaou technique. These were categorized into low grade intra-epithelial lesion/malignancy, high grade intraepithelial lesion/malignancy according to the degree of dyskaryosis. Concentrations of interleukin 8 and interleukin 10 in the samples were determined by enzyme linked immunosorbent assay. RESULTS: the mean age, standard deviation (SD) of the study subjects were 40.6 (7.8) years. A total number of 79 females (92.9%) were negative for intra-epithelial lesion/malignancy (NILM), while 4 (4.71%) and 2 (2.35%) were positive for low grade intra-epithelial lesion/malignancy (LILM) and high grade intra-epithelial lesion (HILM) respectively. While mean levels of interleukin 8 increased with the degree of malignancy, (107.27 ± 11.88pg/ml) in LILM, (114.80 ± 2.12pg/ml) in HILM when compared with NILM (88.39 ± 18.06pg/ml), (f = 0.700, p = 0.018); the mean levels of interleukin 10 was comparable between these groups (p ≥ 0.05). Pearson correlation coefficient analysis showed a negative association between interleukin 8 and interleukin 10 (r = -1.999, p = 0.000) in LILM. CONCLUSION: interleukin 8 cytokines in cervical cancer is associated with the degree of malignancy. Possible anti-inflammatory effect of interleukin 10 was not observed. The African Field Epidemiology Network 2019-03-26 /pmc/articles/PMC6607241/ /pubmed/31303919 http://dx.doi.org/10.11604/pamj.2019.32.148.16314 Text en © Daniel David Osiagwu et al. http://creativecommons.org/licenses/by/2.0/ The Pan African Medical Journal - ISSN 1937-8688. This is an Open Access article distributed under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Osiagwu, Daniel David
Azenabor, Alfred Ederialo
Osijirin, Abimbola Akinduro
Awopetu, Philomina Ikuoyah
Oyegbami, Folasade Ruth
Evaluation of interleukin 8 and interleukin 10 cytokines in liquid based cervical cytology samples
title Evaluation of interleukin 8 and interleukin 10 cytokines in liquid based cervical cytology samples
title_full Evaluation of interleukin 8 and interleukin 10 cytokines in liquid based cervical cytology samples
title_fullStr Evaluation of interleukin 8 and interleukin 10 cytokines in liquid based cervical cytology samples
title_full_unstemmed Evaluation of interleukin 8 and interleukin 10 cytokines in liquid based cervical cytology samples
title_short Evaluation of interleukin 8 and interleukin 10 cytokines in liquid based cervical cytology samples
title_sort evaluation of interleukin 8 and interleukin 10 cytokines in liquid based cervical cytology samples
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6607241/
https://www.ncbi.nlm.nih.gov/pubmed/31303919
http://dx.doi.org/10.11604/pamj.2019.32.148.16314
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