Cargando…

Podocytes and Proteinuria in ANCA-Associated Glomerulonephritis: A Case-Control Study

Proteinuria has been identified as prognosticator of renal outcome in patients with ANCA-associated glomerulonephritis, but whether proteinuria is related to podocyte abnormalities in these patients is largely unknown. We here investigate podocyte foot process width and number of podocytes positive...

Descripción completa

Detalles Bibliográficos
Autores principales: van Daalen, Emma E., Neeskens, Peter, Zandbergen, Malu, Harper, Lorraine, Karras, Alexandre, Vaglio, Augusto, de Zoysa, Janak, Bruijn, Jan A., Bajema, Ingeborg M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6607406/
https://www.ncbi.nlm.nih.gov/pubmed/31297110
http://dx.doi.org/10.3389/fimmu.2019.01405
_version_ 1783432090579107840
author van Daalen, Emma E.
Neeskens, Peter
Zandbergen, Malu
Harper, Lorraine
Karras, Alexandre
Vaglio, Augusto
de Zoysa, Janak
Bruijn, Jan A.
Bajema, Ingeborg M.
author_facet van Daalen, Emma E.
Neeskens, Peter
Zandbergen, Malu
Harper, Lorraine
Karras, Alexandre
Vaglio, Augusto
de Zoysa, Janak
Bruijn, Jan A.
Bajema, Ingeborg M.
author_sort van Daalen, Emma E.
collection PubMed
description Proteinuria has been identified as prognosticator of renal outcome in patients with ANCA-associated glomerulonephritis, but whether proteinuria is related to podocyte abnormalities in these patients is largely unknown. We here investigate podocyte foot process width and number of podocytes positive for the podocyte marker WT-1 in diagnostic renal biopsies of 25 Caucasian patients with ANCA-associated glomerulonephritis in relation to proteinuria. Control tissue was used from pre-transplantation donor kidney biopsies. Proteinuria at 10 weeks follow-up correlated significantly with foot process width (P = 0.04). Biopsies with foot process width ≥600 nm belonged more often to the crescentic or mixed class, whereas biopsies with a foot process width <600 nm were most often categorized as focal class (P = 0.03). The mean number of podocytes based upon expression of WT-1 was significantly lower in patients compared to controls (15 vs. 34 podocytes per glomerulus; P < 0.0001). The significant decrease in expression of the podocyte WT-1 marker in ANCA-associated glomerulonephritis is considered indicative of actual podocyte loss or at least, of a loss of functionality. Furthermore, our study indicates that podocyte foot process width at baseline could be indicative for proteinuria at short term follow up. For prognostic purposes, we therefore suggest to include a description of the foot process width in the diagnostic report of a biopsy with ANCA-associated glomerulonephritis.
format Online
Article
Text
id pubmed-6607406
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-66074062019-07-11 Podocytes and Proteinuria in ANCA-Associated Glomerulonephritis: A Case-Control Study van Daalen, Emma E. Neeskens, Peter Zandbergen, Malu Harper, Lorraine Karras, Alexandre Vaglio, Augusto de Zoysa, Janak Bruijn, Jan A. Bajema, Ingeborg M. Front Immunol Immunology Proteinuria has been identified as prognosticator of renal outcome in patients with ANCA-associated glomerulonephritis, but whether proteinuria is related to podocyte abnormalities in these patients is largely unknown. We here investigate podocyte foot process width and number of podocytes positive for the podocyte marker WT-1 in diagnostic renal biopsies of 25 Caucasian patients with ANCA-associated glomerulonephritis in relation to proteinuria. Control tissue was used from pre-transplantation donor kidney biopsies. Proteinuria at 10 weeks follow-up correlated significantly with foot process width (P = 0.04). Biopsies with foot process width ≥600 nm belonged more often to the crescentic or mixed class, whereas biopsies with a foot process width <600 nm were most often categorized as focal class (P = 0.03). The mean number of podocytes based upon expression of WT-1 was significantly lower in patients compared to controls (15 vs. 34 podocytes per glomerulus; P < 0.0001). The significant decrease in expression of the podocyte WT-1 marker in ANCA-associated glomerulonephritis is considered indicative of actual podocyte loss or at least, of a loss of functionality. Furthermore, our study indicates that podocyte foot process width at baseline could be indicative for proteinuria at short term follow up. For prognostic purposes, we therefore suggest to include a description of the foot process width in the diagnostic report of a biopsy with ANCA-associated glomerulonephritis. Frontiers Media S.A. 2019-06-26 /pmc/articles/PMC6607406/ /pubmed/31297110 http://dx.doi.org/10.3389/fimmu.2019.01405 Text en Copyright © 2019 van Daalen, Neeskens, Zandbergen, Harper, Karras, Vaglio, de Zoysa, Bruijn and Bajema. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
van Daalen, Emma E.
Neeskens, Peter
Zandbergen, Malu
Harper, Lorraine
Karras, Alexandre
Vaglio, Augusto
de Zoysa, Janak
Bruijn, Jan A.
Bajema, Ingeborg M.
Podocytes and Proteinuria in ANCA-Associated Glomerulonephritis: A Case-Control Study
title Podocytes and Proteinuria in ANCA-Associated Glomerulonephritis: A Case-Control Study
title_full Podocytes and Proteinuria in ANCA-Associated Glomerulonephritis: A Case-Control Study
title_fullStr Podocytes and Proteinuria in ANCA-Associated Glomerulonephritis: A Case-Control Study
title_full_unstemmed Podocytes and Proteinuria in ANCA-Associated Glomerulonephritis: A Case-Control Study
title_short Podocytes and Proteinuria in ANCA-Associated Glomerulonephritis: A Case-Control Study
title_sort podocytes and proteinuria in anca-associated glomerulonephritis: a case-control study
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6607406/
https://www.ncbi.nlm.nih.gov/pubmed/31297110
http://dx.doi.org/10.3389/fimmu.2019.01405
work_keys_str_mv AT vandaalenemmae podocytesandproteinuriainancaassociatedglomerulonephritisacasecontrolstudy
AT neeskenspeter podocytesandproteinuriainancaassociatedglomerulonephritisacasecontrolstudy
AT zandbergenmalu podocytesandproteinuriainancaassociatedglomerulonephritisacasecontrolstudy
AT harperlorraine podocytesandproteinuriainancaassociatedglomerulonephritisacasecontrolstudy
AT karrasalexandre podocytesandproteinuriainancaassociatedglomerulonephritisacasecontrolstudy
AT vaglioaugusto podocytesandproteinuriainancaassociatedglomerulonephritisacasecontrolstudy
AT dezoysajanak podocytesandproteinuriainancaassociatedglomerulonephritisacasecontrolstudy
AT bruijnjana podocytesandproteinuriainancaassociatedglomerulonephritisacasecontrolstudy
AT bajemaingeborgm podocytesandproteinuriainancaassociatedglomerulonephritisacasecontrolstudy