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EPR Spectroscopic Examination of Different Types of Paramagnetic Centers in the Blood in the Course of Burn Healing

The multicomponent electron paramagnetic resonance spectra of the blood during healing of skin burned wounds treated with a new generation biodegradable dressings containing poly(lactide-co-glycolide) were analysed. The evolution of different types of paramagnetic centers in the blood with time of h...

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Autores principales: Komosinska-Vassev, Katarzyna, Olczyk, Paweł, Kasperczyk, Janusz, Pilawa, Barbara, Krzyminiewski, Ryszard, Dobosz, Bernadeta, Ramos, Paweł, Stojko, Jerzy, Stojko, Mateusz, Ivanova, Diana, Olczyk, Krystyna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6607714/
https://www.ncbi.nlm.nih.gov/pubmed/31320985
http://dx.doi.org/10.1155/2019/7506274
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author Komosinska-Vassev, Katarzyna
Olczyk, Paweł
Kasperczyk, Janusz
Pilawa, Barbara
Krzyminiewski, Ryszard
Dobosz, Bernadeta
Ramos, Paweł
Stojko, Jerzy
Stojko, Mateusz
Ivanova, Diana
Olczyk, Krystyna
author_facet Komosinska-Vassev, Katarzyna
Olczyk, Paweł
Kasperczyk, Janusz
Pilawa, Barbara
Krzyminiewski, Ryszard
Dobosz, Bernadeta
Ramos, Paweł
Stojko, Jerzy
Stojko, Mateusz
Ivanova, Diana
Olczyk, Krystyna
author_sort Komosinska-Vassev, Katarzyna
collection PubMed
description The multicomponent electron paramagnetic resonance spectra of the blood during healing of skin burned wounds treated with a new generation biodegradable dressings containing poly(lactide-co-glycolide) were analysed. The evolution of different types of paramagnetic centers in the blood with time of healing was determined. The EPR spectra of the blood samples at 230 K temperature were measured at 1, 10, and 21 days after burning of the pig skin. The EPR lines of the following paramagnetic centers: the high-spin Fe(3+) in methemoglobin (line I), high-spin Fe(3+) in transferrin (line II), and Cu(2+) in ceruloplasmin and free radicals (line III) were observed in the X-band (9.3 GHz) spectra of the blood. The multicomponent structure of the EPR spectra of the tested blood samples depended on the time of the healing of the burned wounds. The amount of the high-spin Fe(3+) in methemoglobin (line I) in the blood decreased after 21 days of the healing of the burned wounds. The amount of the high-spin Fe(3+) in transferrin (line II) slightly increased after 21 days of therapy with the basis. The amount of Cu(2+) in ceruloplasmin and free radicals (line III) in the blood was very high after 10 days of therapy. At the first day of the healing of the burned wounds, the highest amount of the high-spin Fe(3+) in methemoglobin (line I), the relatively lower amounts of the high-spin Fe(3+) in transferrin (line II), and Cu(2+) in ceruloplasmin and free radicals (line III) existed in the blood. In the medium phase (after 10 days) of the healing of the burned wounds, the extremely higher amounts of Cu(2+) in ceruloplasmin and free radicals (line III) appeared in the blood. In the last phase (after 21 days), only the low differences between the amounts of the high-spin Fe(3+) in methemoglobin (line I), the high-spin Fe(3+) in transferrin (line II), and Cu(2+) in ceruloplasmin and free radicals (line III) were observed. The present study may serve as a starting point for the development of a new technique for monitoring molecular complexes containing iron Fe(3+) (methemoglobin, transferrin) or copper Cu(2+) ions (ceruloplasmin) and free radicals in the blood during wound healing.
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spelling pubmed-66077142019-07-18 EPR Spectroscopic Examination of Different Types of Paramagnetic Centers in the Blood in the Course of Burn Healing Komosinska-Vassev, Katarzyna Olczyk, Paweł Kasperczyk, Janusz Pilawa, Barbara Krzyminiewski, Ryszard Dobosz, Bernadeta Ramos, Paweł Stojko, Jerzy Stojko, Mateusz Ivanova, Diana Olczyk, Krystyna Oxid Med Cell Longev Research Article The multicomponent electron paramagnetic resonance spectra of the blood during healing of skin burned wounds treated with a new generation biodegradable dressings containing poly(lactide-co-glycolide) were analysed. The evolution of different types of paramagnetic centers in the blood with time of healing was determined. The EPR spectra of the blood samples at 230 K temperature were measured at 1, 10, and 21 days after burning of the pig skin. The EPR lines of the following paramagnetic centers: the high-spin Fe(3+) in methemoglobin (line I), high-spin Fe(3+) in transferrin (line II), and Cu(2+) in ceruloplasmin and free radicals (line III) were observed in the X-band (9.3 GHz) spectra of the blood. The multicomponent structure of the EPR spectra of the tested blood samples depended on the time of the healing of the burned wounds. The amount of the high-spin Fe(3+) in methemoglobin (line I) in the blood decreased after 21 days of the healing of the burned wounds. The amount of the high-spin Fe(3+) in transferrin (line II) slightly increased after 21 days of therapy with the basis. The amount of Cu(2+) in ceruloplasmin and free radicals (line III) in the blood was very high after 10 days of therapy. At the first day of the healing of the burned wounds, the highest amount of the high-spin Fe(3+) in methemoglobin (line I), the relatively lower amounts of the high-spin Fe(3+) in transferrin (line II), and Cu(2+) in ceruloplasmin and free radicals (line III) existed in the blood. In the medium phase (after 10 days) of the healing of the burned wounds, the extremely higher amounts of Cu(2+) in ceruloplasmin and free radicals (line III) appeared in the blood. In the last phase (after 21 days), only the low differences between the amounts of the high-spin Fe(3+) in methemoglobin (line I), the high-spin Fe(3+) in transferrin (line II), and Cu(2+) in ceruloplasmin and free radicals (line III) were observed. The present study may serve as a starting point for the development of a new technique for monitoring molecular complexes containing iron Fe(3+) (methemoglobin, transferrin) or copper Cu(2+) ions (ceruloplasmin) and free radicals in the blood during wound healing. Hindawi 2019-06-19 /pmc/articles/PMC6607714/ /pubmed/31320985 http://dx.doi.org/10.1155/2019/7506274 Text en Copyright © 2019 Katarzyna Komosinska-Vassev et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Komosinska-Vassev, Katarzyna
Olczyk, Paweł
Kasperczyk, Janusz
Pilawa, Barbara
Krzyminiewski, Ryszard
Dobosz, Bernadeta
Ramos, Paweł
Stojko, Jerzy
Stojko, Mateusz
Ivanova, Diana
Olczyk, Krystyna
EPR Spectroscopic Examination of Different Types of Paramagnetic Centers in the Blood in the Course of Burn Healing
title EPR Spectroscopic Examination of Different Types of Paramagnetic Centers in the Blood in the Course of Burn Healing
title_full EPR Spectroscopic Examination of Different Types of Paramagnetic Centers in the Blood in the Course of Burn Healing
title_fullStr EPR Spectroscopic Examination of Different Types of Paramagnetic Centers in the Blood in the Course of Burn Healing
title_full_unstemmed EPR Spectroscopic Examination of Different Types of Paramagnetic Centers in the Blood in the Course of Burn Healing
title_short EPR Spectroscopic Examination of Different Types of Paramagnetic Centers in the Blood in the Course of Burn Healing
title_sort epr spectroscopic examination of different types of paramagnetic centers in the blood in the course of burn healing
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6607714/
https://www.ncbi.nlm.nih.gov/pubmed/31320985
http://dx.doi.org/10.1155/2019/7506274
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