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Undergraduates Phenotyping Arabidopsis Knockouts in a Course-Based Undergraduate Research Experience: Exploring Plant Fitness and Vigor Using Quantitative Phenotyping Methods

We present a curriculum description, an initial student outcome investigation, and sample scientific results for a representative Course-Based Undergraduate Research Experience (CURE) that is part of the “Undergraduates Phenotyping Arabidopsis Knockouts” (unPAK) network. CUREs in the unPAK network c...

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Autores principales: Murren, Courtney J., Wolyniak, Michael J., Rutter, Matthew T., Bisner, April M., Callahan, Hilary S., Strand, Allan E., Corwin, Lisa A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Microbiology 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6608610/
https://www.ncbi.nlm.nih.gov/pubmed/31316686
http://dx.doi.org/10.1128/jmbe.v20i2.1650
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author Murren, Courtney J.
Wolyniak, Michael J.
Rutter, Matthew T.
Bisner, April M.
Callahan, Hilary S.
Strand, Allan E.
Corwin, Lisa A.
author_facet Murren, Courtney J.
Wolyniak, Michael J.
Rutter, Matthew T.
Bisner, April M.
Callahan, Hilary S.
Strand, Allan E.
Corwin, Lisa A.
author_sort Murren, Courtney J.
collection PubMed
description We present a curriculum description, an initial student outcome investigation, and sample scientific results for a representative Course-Based Undergraduate Research Experience (CURE) that is part of the “Undergraduates Phenotyping Arabidopsis Knockouts” (unPAK) network. CUREs in the unPAK network characterize quantitative phenotypes of the model plant Arabidopsis from across environments to uncover connections between genotype and phenotype. Students in unPAK CUREs grow plants in a replicated block design and make quantitative measurements throughout the semester. This CURE enables students to answer plant science questions that draw from fields such as environmental science, genetics, ecology, and evolution. Findings indicate that this experience provides students with opportunities to make relevant scientific discoveries. Eighty percent of student datasets produced from the CURE met criteria for inclusion in the project database, indicative of student learning in data collection and analysis of quantitative plant traits. Student datasets uncovered novel effects of mutation on plant form. In addition, students’ science self-efficacy increased as a result of course participation, and faculty feedback on course implementation was positive. We present unPAK as a new network that supports CUREs and research experiences focused on collecting biological data made publicly available to the scientific community. The unPAK CUREs can be tailored to address instructor interests or pedagogical needs while involving students in research investigating quantitative plant phenotypes.
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spelling pubmed-66086102019-07-17 Undergraduates Phenotyping Arabidopsis Knockouts in a Course-Based Undergraduate Research Experience: Exploring Plant Fitness and Vigor Using Quantitative Phenotyping Methods Murren, Courtney J. Wolyniak, Michael J. Rutter, Matthew T. Bisner, April M. Callahan, Hilary S. Strand, Allan E. Corwin, Lisa A. J Microbiol Biol Educ Curriculum We present a curriculum description, an initial student outcome investigation, and sample scientific results for a representative Course-Based Undergraduate Research Experience (CURE) that is part of the “Undergraduates Phenotyping Arabidopsis Knockouts” (unPAK) network. CUREs in the unPAK network characterize quantitative phenotypes of the model plant Arabidopsis from across environments to uncover connections between genotype and phenotype. Students in unPAK CUREs grow plants in a replicated block design and make quantitative measurements throughout the semester. This CURE enables students to answer plant science questions that draw from fields such as environmental science, genetics, ecology, and evolution. Findings indicate that this experience provides students with opportunities to make relevant scientific discoveries. Eighty percent of student datasets produced from the CURE met criteria for inclusion in the project database, indicative of student learning in data collection and analysis of quantitative plant traits. Student datasets uncovered novel effects of mutation on plant form. In addition, students’ science self-efficacy increased as a result of course participation, and faculty feedback on course implementation was positive. We present unPAK as a new network that supports CUREs and research experiences focused on collecting biological data made publicly available to the scientific community. The unPAK CUREs can be tailored to address instructor interests or pedagogical needs while involving students in research investigating quantitative plant phenotypes. American Society of Microbiology 2019-06-28 /pmc/articles/PMC6608610/ /pubmed/31316686 http://dx.doi.org/10.1128/jmbe.v20i2.1650 Text en ©2019 Author(s). Published by the American Society for Microbiology This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial-NoDerivatives 4.0 International license (https://creativecommons.org/licenses/by-nc-nd/4.0/ and https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode), which grants the public the nonexclusive right to copy, distribute, or display the published work.
spellingShingle Curriculum
Murren, Courtney J.
Wolyniak, Michael J.
Rutter, Matthew T.
Bisner, April M.
Callahan, Hilary S.
Strand, Allan E.
Corwin, Lisa A.
Undergraduates Phenotyping Arabidopsis Knockouts in a Course-Based Undergraduate Research Experience: Exploring Plant Fitness and Vigor Using Quantitative Phenotyping Methods
title Undergraduates Phenotyping Arabidopsis Knockouts in a Course-Based Undergraduate Research Experience: Exploring Plant Fitness and Vigor Using Quantitative Phenotyping Methods
title_full Undergraduates Phenotyping Arabidopsis Knockouts in a Course-Based Undergraduate Research Experience: Exploring Plant Fitness and Vigor Using Quantitative Phenotyping Methods
title_fullStr Undergraduates Phenotyping Arabidopsis Knockouts in a Course-Based Undergraduate Research Experience: Exploring Plant Fitness and Vigor Using Quantitative Phenotyping Methods
title_full_unstemmed Undergraduates Phenotyping Arabidopsis Knockouts in a Course-Based Undergraduate Research Experience: Exploring Plant Fitness and Vigor Using Quantitative Phenotyping Methods
title_short Undergraduates Phenotyping Arabidopsis Knockouts in a Course-Based Undergraduate Research Experience: Exploring Plant Fitness and Vigor Using Quantitative Phenotyping Methods
title_sort undergraduates phenotyping arabidopsis knockouts in a course-based undergraduate research experience: exploring plant fitness and vigor using quantitative phenotyping methods
topic Curriculum
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6608610/
https://www.ncbi.nlm.nih.gov/pubmed/31316686
http://dx.doi.org/10.1128/jmbe.v20i2.1650
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