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Formation of a highly dense tetra-rhenium cluster in a protein crystal and its implications in medical imaging
The fact that a protein crystal can serve as a chemical reaction vessel is intrinsically fascinating. That it can produce an electron-dense tetranuclear rhenium cluster compound from a rhenium tricarbonyl tribromo starting compound adds to the fascination. Such a cluster has been synthesized previ...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6608631/ https://www.ncbi.nlm.nih.gov/pubmed/31316813 http://dx.doi.org/10.1107/S2052252519006651 |
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author | Brink, Alice Helliwell, John R. |
author_facet | Brink, Alice Helliwell, John R. |
author_sort | Brink, Alice |
collection | PubMed |
description | The fact that a protein crystal can serve as a chemical reaction vessel is intrinsically fascinating. That it can produce an electron-dense tetranuclear rhenium cluster compound from a rhenium tricarbonyl tribromo starting compound adds to the fascination. Such a cluster has been synthesized previously in vitro, where it formed under basic conditions. Therefore, its synthesis in a protein crystal grown at pH 4.5 is even more unexpected. The X-ray crystal structures presented here are for the protein hen egg-white lysozyme incubated with a rhenium tricarbonyl tribromo compound for periods of one and two years. These reveal a completed, very well resolved, tetra-rhenium cluster after two years and an intermediate state, where the carbonyl ligands to the rhenium cluster are not yet clearly resolved, after one year. A dense tetranuclear rhenium cluster, and its technetium form, offer enhanced contrast in medical imaging. Stimulated by these crystallography results, the unusual formation of such a species directly in an in vivo situation has been considered. It offers a new option for medical imaging compounds, particularly when considering the application of the pre-formed tetranuclear cluster, suggesting that it may be suitable for medical diagnosis because of its stability, preference of formation and biological compatibility. |
format | Online Article Text |
id | pubmed-6608631 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-66086312019-07-17 Formation of a highly dense tetra-rhenium cluster in a protein crystal and its implications in medical imaging Brink, Alice Helliwell, John R. IUCrJ Research Papers The fact that a protein crystal can serve as a chemical reaction vessel is intrinsically fascinating. That it can produce an electron-dense tetranuclear rhenium cluster compound from a rhenium tricarbonyl tribromo starting compound adds to the fascination. Such a cluster has been synthesized previously in vitro, where it formed under basic conditions. Therefore, its synthesis in a protein crystal grown at pH 4.5 is even more unexpected. The X-ray crystal structures presented here are for the protein hen egg-white lysozyme incubated with a rhenium tricarbonyl tribromo compound for periods of one and two years. These reveal a completed, very well resolved, tetra-rhenium cluster after two years and an intermediate state, where the carbonyl ligands to the rhenium cluster are not yet clearly resolved, after one year. A dense tetranuclear rhenium cluster, and its technetium form, offer enhanced contrast in medical imaging. Stimulated by these crystallography results, the unusual formation of such a species directly in an in vivo situation has been considered. It offers a new option for medical imaging compounds, particularly when considering the application of the pre-formed tetranuclear cluster, suggesting that it may be suitable for medical diagnosis because of its stability, preference of formation and biological compatibility. International Union of Crystallography 2019-06-13 /pmc/articles/PMC6608631/ /pubmed/31316813 http://dx.doi.org/10.1107/S2052252519006651 Text en © Brink & Helliwell 2019 http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Research Papers Brink, Alice Helliwell, John R. Formation of a highly dense tetra-rhenium cluster in a protein crystal and its implications in medical imaging |
title | Formation of a highly dense tetra-rhenium cluster in a protein crystal and its implications in medical imaging |
title_full | Formation of a highly dense tetra-rhenium cluster in a protein crystal and its implications in medical imaging |
title_fullStr | Formation of a highly dense tetra-rhenium cluster in a protein crystal and its implications in medical imaging |
title_full_unstemmed | Formation of a highly dense tetra-rhenium cluster in a protein crystal and its implications in medical imaging |
title_short | Formation of a highly dense tetra-rhenium cluster in a protein crystal and its implications in medical imaging |
title_sort | formation of a highly dense tetra-rhenium cluster in a protein crystal and its implications in medical imaging |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6608631/ https://www.ncbi.nlm.nih.gov/pubmed/31316813 http://dx.doi.org/10.1107/S2052252519006651 |
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