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Room-temperature photo-induced martensitic transformation in a protein crystal

Martensitic transformations are the first-order crystal-to-crystal phase transitions that occur mostly in materials such as steel, alloys and ceramics, thus having many technological applications. These phase transitions are rarely observed in molecular crystals and have not been detected in protein...

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Autores principales: Dajnowicz, Steven, Langan, Patricia S., Weiss, Kevin L., Ivanov, Ilia N., Kovalevsky, Andrey
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6608640/
https://www.ncbi.nlm.nih.gov/pubmed/31316806
http://dx.doi.org/10.1107/S2052252519005761
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author Dajnowicz, Steven
Langan, Patricia S.
Weiss, Kevin L.
Ivanov, Ilia N.
Kovalevsky, Andrey
author_facet Dajnowicz, Steven
Langan, Patricia S.
Weiss, Kevin L.
Ivanov, Ilia N.
Kovalevsky, Andrey
author_sort Dajnowicz, Steven
collection PubMed
description Martensitic transformations are the first-order crystal-to-crystal phase transitions that occur mostly in materials such as steel, alloys and ceramics, thus having many technological applications. These phase transitions are rarely observed in molecular crystals and have not been detected in protein crystals. Reversibly switchable fluorescent proteins are widely used in biotechnology, including super-resolution molecular imaging, and hold promise as candidate biomaterials for future high-tech applications. Here, we report on a reversibly switchable fluorescent protein, Tetdron, whose crystals undergo a photo-induced martensitic transformation at room temperature. Room-temperature X-ray crystallography demonstrates that at equilibrium Tetdron chromophores are all in the trans configuration, with an ∼1:1 mixture of their protonated and deprotonated forms. Irradiation of a Tetdron crystal with 400 nm light induces a martensitic transformation, which results in Tetdron tetramerization at room temperature revealed by X-ray photocrystallography. Crystal and solution spectroscopic measurements provide evidence that the photo-induced martensitic phase transition is coupled with the chromophore deprotonation, but no trans–cis isomerization is detected in the structure of an irradiated crystal. It is hypothesized that protein dynamics assists in the light-induced proton transfer from the chromophore to the bulk solvent and in the ensuing martensitic phase transition. The unique properties of Tetdron may be useful in developing novel biomaterials for optogenetics, data storage and nanotechnology.
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spelling pubmed-66086402019-07-17 Room-temperature photo-induced martensitic transformation in a protein crystal Dajnowicz, Steven Langan, Patricia S. Weiss, Kevin L. Ivanov, Ilia N. Kovalevsky, Andrey IUCrJ Research Papers Martensitic transformations are the first-order crystal-to-crystal phase transitions that occur mostly in materials such as steel, alloys and ceramics, thus having many technological applications. These phase transitions are rarely observed in molecular crystals and have not been detected in protein crystals. Reversibly switchable fluorescent proteins are widely used in biotechnology, including super-resolution molecular imaging, and hold promise as candidate biomaterials for future high-tech applications. Here, we report on a reversibly switchable fluorescent protein, Tetdron, whose crystals undergo a photo-induced martensitic transformation at room temperature. Room-temperature X-ray crystallography demonstrates that at equilibrium Tetdron chromophores are all in the trans configuration, with an ∼1:1 mixture of their protonated and deprotonated forms. Irradiation of a Tetdron crystal with 400 nm light induces a martensitic transformation, which results in Tetdron tetramerization at room temperature revealed by X-ray photocrystallography. Crystal and solution spectroscopic measurements provide evidence that the photo-induced martensitic phase transition is coupled with the chromophore deprotonation, but no trans–cis isomerization is detected in the structure of an irradiated crystal. It is hypothesized that protein dynamics assists in the light-induced proton transfer from the chromophore to the bulk solvent and in the ensuing martensitic phase transition. The unique properties of Tetdron may be useful in developing novel biomaterials for optogenetics, data storage and nanotechnology. International Union of Crystallography 2019-05-22 /pmc/articles/PMC6608640/ /pubmed/31316806 http://dx.doi.org/10.1107/S2052252519005761 Text en © Dajnowicz et al. 2019 http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.http://creativecommons.org/licenses/by/4.0/
spellingShingle Research Papers
Dajnowicz, Steven
Langan, Patricia S.
Weiss, Kevin L.
Ivanov, Ilia N.
Kovalevsky, Andrey
Room-temperature photo-induced martensitic transformation in a protein crystal
title Room-temperature photo-induced martensitic transformation in a protein crystal
title_full Room-temperature photo-induced martensitic transformation in a protein crystal
title_fullStr Room-temperature photo-induced martensitic transformation in a protein crystal
title_full_unstemmed Room-temperature photo-induced martensitic transformation in a protein crystal
title_short Room-temperature photo-induced martensitic transformation in a protein crystal
title_sort room-temperature photo-induced martensitic transformation in a protein crystal
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6608640/
https://www.ncbi.nlm.nih.gov/pubmed/31316806
http://dx.doi.org/10.1107/S2052252519005761
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