Enhanced Biosorption of Nickel Ions on Immobilized Surface-Engineered Yeast Using Nickel-Binding Peptides

Three nickel-binding peptides were screened from a phage peptide library and displayed separately or in combination with surface-engineered Saccharomyces cerevisiae EBY100. The sorption of nickel ions on the surface of yeast cells increased with the increasing number of nickel Ni(II)-binding peptide...

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Detalles Bibliográficos
Autores principales: Li, Hua, Dong, Wei, Liu, Yong, Zhang, Haiyan, Wang, Gang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6608678/
https://www.ncbi.nlm.nih.gov/pubmed/31297097
http://dx.doi.org/10.3389/fmicb.2019.01254
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author Li, Hua
Dong, Wei
Liu, Yong
Zhang, Haiyan
Wang, Gang
author_facet Li, Hua
Dong, Wei
Liu, Yong
Zhang, Haiyan
Wang, Gang
author_sort Li, Hua
collection PubMed
description Three nickel-binding peptides were screened from a phage peptide library and displayed separately or in combination with surface-engineered Saccharomyces cerevisiae EBY100. The sorption of nickel ions on the surface of yeast cells increased with the increasing number of nickel Ni(II)-binding peptides displayed. The combined expression of the three peptides by EBY100/pYD1-N123 demonstrated the highest sorption of Ni(II) (2.603 ± 0.004 g g(−1), dry weight) and an enhanced sorption capacity of 60.15%, compared to S. cerevisiae EBY100. An orthogonal test for yeast immobilization was designed. A maximum sorption capability of 68.62% was observed for a treatment at 25°C with 2.0% calcium chloride and 3.0% sodium alginate.
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spelling pubmed-66086782019-07-11 Enhanced Biosorption of Nickel Ions on Immobilized Surface-Engineered Yeast Using Nickel-Binding Peptides Li, Hua Dong, Wei Liu, Yong Zhang, Haiyan Wang, Gang Front Microbiol Microbiology Three nickel-binding peptides were screened from a phage peptide library and displayed separately or in combination with surface-engineered Saccharomyces cerevisiae EBY100. The sorption of nickel ions on the surface of yeast cells increased with the increasing number of nickel Ni(II)-binding peptides displayed. The combined expression of the three peptides by EBY100/pYD1-N123 demonstrated the highest sorption of Ni(II) (2.603 ± 0.004 g g(−1), dry weight) and an enhanced sorption capacity of 60.15%, compared to S. cerevisiae EBY100. An orthogonal test for yeast immobilization was designed. A maximum sorption capability of 68.62% was observed for a treatment at 25°C with 2.0% calcium chloride and 3.0% sodium alginate. Frontiers Media S.A. 2019-06-26 /pmc/articles/PMC6608678/ /pubmed/31297097 http://dx.doi.org/10.3389/fmicb.2019.01254 Text en Copyright © 2019 Li, Dong, Liu, Zhang and Wang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Li, Hua
Dong, Wei
Liu, Yong
Zhang, Haiyan
Wang, Gang
Enhanced Biosorption of Nickel Ions on Immobilized Surface-Engineered Yeast Using Nickel-Binding Peptides
title Enhanced Biosorption of Nickel Ions on Immobilized Surface-Engineered Yeast Using Nickel-Binding Peptides
title_full Enhanced Biosorption of Nickel Ions on Immobilized Surface-Engineered Yeast Using Nickel-Binding Peptides
title_fullStr Enhanced Biosorption of Nickel Ions on Immobilized Surface-Engineered Yeast Using Nickel-Binding Peptides
title_full_unstemmed Enhanced Biosorption of Nickel Ions on Immobilized Surface-Engineered Yeast Using Nickel-Binding Peptides
title_short Enhanced Biosorption of Nickel Ions on Immobilized Surface-Engineered Yeast Using Nickel-Binding Peptides
title_sort enhanced biosorption of nickel ions on immobilized surface-engineered yeast using nickel-binding peptides
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6608678/
https://www.ncbi.nlm.nih.gov/pubmed/31297097
http://dx.doi.org/10.3389/fmicb.2019.01254
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