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Purpurogallin Protects Keratinocytes from Damage and Apoptosis Induced by Ultraviolet B Radiation and Particulate Matter 2.5
Purpurogallin, a natural phenol obtained from oak nutgalls, has been shown to possess antioxidant, anticancer, and anti-inflammatory effects. Recently, in addition to ultraviolet B (UVB) radiation that induces cell apoptosis via oxidative stress, particulate matter 2.5 (PM(2.5)) was shown to trigger...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society of Applied Pharmacology
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6609104/ https://www.ncbi.nlm.nih.gov/pubmed/30419635 http://dx.doi.org/10.4062/biomolther.2018.151 |
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author | Zhen, Ao Xuan Piao, Mei Jing Hyun, Yu Jae Kang, Kyoung Ah Ryu, Yea Seong Cho, Suk Ju Kang, Hee Kyoung Koh, Young Sang Ahn, Mee Jung Kim, Tae Hoon Hyun, Jin Won |
author_facet | Zhen, Ao Xuan Piao, Mei Jing Hyun, Yu Jae Kang, Kyoung Ah Ryu, Yea Seong Cho, Suk Ju Kang, Hee Kyoung Koh, Young Sang Ahn, Mee Jung Kim, Tae Hoon Hyun, Jin Won |
author_sort | Zhen, Ao Xuan |
collection | PubMed |
description | Purpurogallin, a natural phenol obtained from oak nutgalls, has been shown to possess antioxidant, anticancer, and anti-inflammatory effects. Recently, in addition to ultraviolet B (UVB) radiation that induces cell apoptosis via oxidative stress, particulate matter 2.5 (PM(2.5)) was shown to trigger excessive production of reactive oxygen species. In this study, we observed that UVB radiation and PM(2.5) severely damaged human HaCaT keratinocytes, disrupting cellular DNA, lipids, and proteins and causing mitochondrial depolarization. Purpurogallin protected HaCaT cells from apoptosis induced by UVB radiation and/or PM(2.5). Furthermore, purpurogallin effectively modulates the pro-apoptotic and anti-apoptotic proteins under UVB irradiation via caspase signaling pathways. Additionally, purpurogallin reduced apoptosis via MAPK signaling pathways, as demonstrated using MAPK-p38, ERK, and JNK inhibitors. These results indicate that purpurogallin possesses antioxidant effects and protects cells from damage and apoptosis induced by UVB radiation and PM(2.5). |
format | Online Article Text |
id | pubmed-6609104 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The Korean Society of Applied Pharmacology |
record_format | MEDLINE/PubMed |
spelling | pubmed-66091042019-07-09 Purpurogallin Protects Keratinocytes from Damage and Apoptosis Induced by Ultraviolet B Radiation and Particulate Matter 2.5 Zhen, Ao Xuan Piao, Mei Jing Hyun, Yu Jae Kang, Kyoung Ah Ryu, Yea Seong Cho, Suk Ju Kang, Hee Kyoung Koh, Young Sang Ahn, Mee Jung Kim, Tae Hoon Hyun, Jin Won Biomol Ther (Seoul) Original Article Purpurogallin, a natural phenol obtained from oak nutgalls, has been shown to possess antioxidant, anticancer, and anti-inflammatory effects. Recently, in addition to ultraviolet B (UVB) radiation that induces cell apoptosis via oxidative stress, particulate matter 2.5 (PM(2.5)) was shown to trigger excessive production of reactive oxygen species. In this study, we observed that UVB radiation and PM(2.5) severely damaged human HaCaT keratinocytes, disrupting cellular DNA, lipids, and proteins and causing mitochondrial depolarization. Purpurogallin protected HaCaT cells from apoptosis induced by UVB radiation and/or PM(2.5). Furthermore, purpurogallin effectively modulates the pro-apoptotic and anti-apoptotic proteins under UVB irradiation via caspase signaling pathways. Additionally, purpurogallin reduced apoptosis via MAPK signaling pathways, as demonstrated using MAPK-p38, ERK, and JNK inhibitors. These results indicate that purpurogallin possesses antioxidant effects and protects cells from damage and apoptosis induced by UVB radiation and PM(2.5). The Korean Society of Applied Pharmacology 2019-07 2018-11-12 /pmc/articles/PMC6609104/ /pubmed/30419635 http://dx.doi.org/10.4062/biomolther.2018.151 Text en Copyright ©2019, The Korean Society of Applied Pharmacology http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Zhen, Ao Xuan Piao, Mei Jing Hyun, Yu Jae Kang, Kyoung Ah Ryu, Yea Seong Cho, Suk Ju Kang, Hee Kyoung Koh, Young Sang Ahn, Mee Jung Kim, Tae Hoon Hyun, Jin Won Purpurogallin Protects Keratinocytes from Damage and Apoptosis Induced by Ultraviolet B Radiation and Particulate Matter 2.5 |
title | Purpurogallin Protects Keratinocytes from Damage and Apoptosis Induced by Ultraviolet B Radiation and Particulate Matter 2.5 |
title_full | Purpurogallin Protects Keratinocytes from Damage and Apoptosis Induced by Ultraviolet B Radiation and Particulate Matter 2.5 |
title_fullStr | Purpurogallin Protects Keratinocytes from Damage and Apoptosis Induced by Ultraviolet B Radiation and Particulate Matter 2.5 |
title_full_unstemmed | Purpurogallin Protects Keratinocytes from Damage and Apoptosis Induced by Ultraviolet B Radiation and Particulate Matter 2.5 |
title_short | Purpurogallin Protects Keratinocytes from Damage and Apoptosis Induced by Ultraviolet B Radiation and Particulate Matter 2.5 |
title_sort | purpurogallin protects keratinocytes from damage and apoptosis induced by ultraviolet b radiation and particulate matter 2.5 |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6609104/ https://www.ncbi.nlm.nih.gov/pubmed/30419635 http://dx.doi.org/10.4062/biomolther.2018.151 |
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