Cargando…

Purpurogallin Protects Keratinocytes from Damage and Apoptosis Induced by Ultraviolet B Radiation and Particulate Matter 2.5

Purpurogallin, a natural phenol obtained from oak nutgalls, has been shown to possess antioxidant, anticancer, and anti-inflammatory effects. Recently, in addition to ultraviolet B (UVB) radiation that induces cell apoptosis via oxidative stress, particulate matter 2.5 (PM(2.5)) was shown to trigger...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhen, Ao Xuan, Piao, Mei Jing, Hyun, Yu Jae, Kang, Kyoung Ah, Ryu, Yea Seong, Cho, Suk Ju, Kang, Hee Kyoung, Koh, Young Sang, Ahn, Mee Jung, Kim, Tae Hoon, Hyun, Jin Won
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Applied Pharmacology 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6609104/
https://www.ncbi.nlm.nih.gov/pubmed/30419635
http://dx.doi.org/10.4062/biomolther.2018.151
_version_ 1783432255283134464
author Zhen, Ao Xuan
Piao, Mei Jing
Hyun, Yu Jae
Kang, Kyoung Ah
Ryu, Yea Seong
Cho, Suk Ju
Kang, Hee Kyoung
Koh, Young Sang
Ahn, Mee Jung
Kim, Tae Hoon
Hyun, Jin Won
author_facet Zhen, Ao Xuan
Piao, Mei Jing
Hyun, Yu Jae
Kang, Kyoung Ah
Ryu, Yea Seong
Cho, Suk Ju
Kang, Hee Kyoung
Koh, Young Sang
Ahn, Mee Jung
Kim, Tae Hoon
Hyun, Jin Won
author_sort Zhen, Ao Xuan
collection PubMed
description Purpurogallin, a natural phenol obtained from oak nutgalls, has been shown to possess antioxidant, anticancer, and anti-inflammatory effects. Recently, in addition to ultraviolet B (UVB) radiation that induces cell apoptosis via oxidative stress, particulate matter 2.5 (PM(2.5)) was shown to trigger excessive production of reactive oxygen species. In this study, we observed that UVB radiation and PM(2.5) severely damaged human HaCaT keratinocytes, disrupting cellular DNA, lipids, and proteins and causing mitochondrial depolarization. Purpurogallin protected HaCaT cells from apoptosis induced by UVB radiation and/or PM(2.5). Furthermore, purpurogallin effectively modulates the pro-apoptotic and anti-apoptotic proteins under UVB irradiation via caspase signaling pathways. Additionally, purpurogallin reduced apoptosis via MAPK signaling pathways, as demonstrated using MAPK-p38, ERK, and JNK inhibitors. These results indicate that purpurogallin possesses antioxidant effects and protects cells from damage and apoptosis induced by UVB radiation and PM(2.5).
format Online
Article
Text
id pubmed-6609104
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher The Korean Society of Applied Pharmacology
record_format MEDLINE/PubMed
spelling pubmed-66091042019-07-09 Purpurogallin Protects Keratinocytes from Damage and Apoptosis Induced by Ultraviolet B Radiation and Particulate Matter 2.5 Zhen, Ao Xuan Piao, Mei Jing Hyun, Yu Jae Kang, Kyoung Ah Ryu, Yea Seong Cho, Suk Ju Kang, Hee Kyoung Koh, Young Sang Ahn, Mee Jung Kim, Tae Hoon Hyun, Jin Won Biomol Ther (Seoul) Original Article Purpurogallin, a natural phenol obtained from oak nutgalls, has been shown to possess antioxidant, anticancer, and anti-inflammatory effects. Recently, in addition to ultraviolet B (UVB) radiation that induces cell apoptosis via oxidative stress, particulate matter 2.5 (PM(2.5)) was shown to trigger excessive production of reactive oxygen species. In this study, we observed that UVB radiation and PM(2.5) severely damaged human HaCaT keratinocytes, disrupting cellular DNA, lipids, and proteins and causing mitochondrial depolarization. Purpurogallin protected HaCaT cells from apoptosis induced by UVB radiation and/or PM(2.5). Furthermore, purpurogallin effectively modulates the pro-apoptotic and anti-apoptotic proteins under UVB irradiation via caspase signaling pathways. Additionally, purpurogallin reduced apoptosis via MAPK signaling pathways, as demonstrated using MAPK-p38, ERK, and JNK inhibitors. These results indicate that purpurogallin possesses antioxidant effects and protects cells from damage and apoptosis induced by UVB radiation and PM(2.5). The Korean Society of Applied Pharmacology 2019-07 2018-11-12 /pmc/articles/PMC6609104/ /pubmed/30419635 http://dx.doi.org/10.4062/biomolther.2018.151 Text en Copyright ©2019, The Korean Society of Applied Pharmacology http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Zhen, Ao Xuan
Piao, Mei Jing
Hyun, Yu Jae
Kang, Kyoung Ah
Ryu, Yea Seong
Cho, Suk Ju
Kang, Hee Kyoung
Koh, Young Sang
Ahn, Mee Jung
Kim, Tae Hoon
Hyun, Jin Won
Purpurogallin Protects Keratinocytes from Damage and Apoptosis Induced by Ultraviolet B Radiation and Particulate Matter 2.5
title Purpurogallin Protects Keratinocytes from Damage and Apoptosis Induced by Ultraviolet B Radiation and Particulate Matter 2.5
title_full Purpurogallin Protects Keratinocytes from Damage and Apoptosis Induced by Ultraviolet B Radiation and Particulate Matter 2.5
title_fullStr Purpurogallin Protects Keratinocytes from Damage and Apoptosis Induced by Ultraviolet B Radiation and Particulate Matter 2.5
title_full_unstemmed Purpurogallin Protects Keratinocytes from Damage and Apoptosis Induced by Ultraviolet B Radiation and Particulate Matter 2.5
title_short Purpurogallin Protects Keratinocytes from Damage and Apoptosis Induced by Ultraviolet B Radiation and Particulate Matter 2.5
title_sort purpurogallin protects keratinocytes from damage and apoptosis induced by ultraviolet b radiation and particulate matter 2.5
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6609104/
https://www.ncbi.nlm.nih.gov/pubmed/30419635
http://dx.doi.org/10.4062/biomolther.2018.151
work_keys_str_mv AT zhenaoxuan purpurogallinprotectskeratinocytesfromdamageandapoptosisinducedbyultravioletbradiationandparticulatematter25
AT piaomeijing purpurogallinprotectskeratinocytesfromdamageandapoptosisinducedbyultravioletbradiationandparticulatematter25
AT hyunyujae purpurogallinprotectskeratinocytesfromdamageandapoptosisinducedbyultravioletbradiationandparticulatematter25
AT kangkyoungah purpurogallinprotectskeratinocytesfromdamageandapoptosisinducedbyultravioletbradiationandparticulatematter25
AT ryuyeaseong purpurogallinprotectskeratinocytesfromdamageandapoptosisinducedbyultravioletbradiationandparticulatematter25
AT chosukju purpurogallinprotectskeratinocytesfromdamageandapoptosisinducedbyultravioletbradiationandparticulatematter25
AT kangheekyoung purpurogallinprotectskeratinocytesfromdamageandapoptosisinducedbyultravioletbradiationandparticulatematter25
AT kohyoungsang purpurogallinprotectskeratinocytesfromdamageandapoptosisinducedbyultravioletbradiationandparticulatematter25
AT ahnmeejung purpurogallinprotectskeratinocytesfromdamageandapoptosisinducedbyultravioletbradiationandparticulatematter25
AT kimtaehoon purpurogallinprotectskeratinocytesfromdamageandapoptosisinducedbyultravioletbradiationandparticulatematter25
AT hyunjinwon purpurogallinprotectskeratinocytesfromdamageandapoptosisinducedbyultravioletbradiationandparticulatematter25