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Short-term sleep fragmentation enhances anxiety-related behavior: The role of hormonal alterations

INTRODUCTION: The neuroendocrine background of acute sleep fragmentation in obstructive sleep apnea and sleep fragmentation involvement in psychiatric comorbidities, common in these patients, are still largely unknown. The aim of this study was to determine the effects of short-term experimental sle...

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Autores principales: Grubac, Zeljko, Sutulovic, Nikola, Ademovic, Anida, Velimirovic, Milica, Rasic-Markovic, Aleksandra, Macut, Djuro, Petronijevic, Natasa, Stanojlovic, Olivera, Hrncic, Dragan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6609147/
https://www.ncbi.nlm.nih.gov/pubmed/31269081
http://dx.doi.org/10.1371/journal.pone.0218920
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author Grubac, Zeljko
Sutulovic, Nikola
Ademovic, Anida
Velimirovic, Milica
Rasic-Markovic, Aleksandra
Macut, Djuro
Petronijevic, Natasa
Stanojlovic, Olivera
Hrncic, Dragan
author_facet Grubac, Zeljko
Sutulovic, Nikola
Ademovic, Anida
Velimirovic, Milica
Rasic-Markovic, Aleksandra
Macut, Djuro
Petronijevic, Natasa
Stanojlovic, Olivera
Hrncic, Dragan
author_sort Grubac, Zeljko
collection PubMed
description INTRODUCTION: The neuroendocrine background of acute sleep fragmentation in obstructive sleep apnea and sleep fragmentation involvement in psychiatric comorbidities, common in these patients, are still largely unknown. The aim of this study was to determine the effects of short-term experimental sleep fragmentation on anxiety -like behavior and hormonal status in rats. METHODS: Male rats were adapted to treadmill (ON and OFF mode with belt speed set on 0.02m/s and 0.00m/s) and randomized to: 1) treadmill control (TC, only OFF mode); 2) motion, activity control (AC, 10min ON and 30min OFF mode) and 3) sleep fragmentation (SF, 30s ON and 90s OFF mode) group. Six hours later, the animals were tested in the open field, elevated plus maze and light/dark test (n = 8/group). Testosterone, estradiol, progesterone and corticosterone were determined in separate animal cohort immediately upon sleep fragmentation (n = 6/group). RESULTS: SF rats showed decreased rearings number, decreased time spent in the central area and increased thigmotaxic index compared to TC and AC rats in the open field test. Similarly, increased anxiety upon sleep fragmentation was observed in the elevated plus maze and the light/dark test. Significantly lower testosterone, estradiol and progesterone levels were determined in SF in comparison to AC and TC groups, while there was no significant difference in the levels of corticosterone. CONCLUSION: Short term sleep fragmentation enhances anxiety-related behavior in rats, which could be partly mediated by the observed hormonal changes presented in the current study in form of testosterone, estradiol and progesterone depletion.
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spelling pubmed-66091472019-07-12 Short-term sleep fragmentation enhances anxiety-related behavior: The role of hormonal alterations Grubac, Zeljko Sutulovic, Nikola Ademovic, Anida Velimirovic, Milica Rasic-Markovic, Aleksandra Macut, Djuro Petronijevic, Natasa Stanojlovic, Olivera Hrncic, Dragan PLoS One Research Article INTRODUCTION: The neuroendocrine background of acute sleep fragmentation in obstructive sleep apnea and sleep fragmentation involvement in psychiatric comorbidities, common in these patients, are still largely unknown. The aim of this study was to determine the effects of short-term experimental sleep fragmentation on anxiety -like behavior and hormonal status in rats. METHODS: Male rats were adapted to treadmill (ON and OFF mode with belt speed set on 0.02m/s and 0.00m/s) and randomized to: 1) treadmill control (TC, only OFF mode); 2) motion, activity control (AC, 10min ON and 30min OFF mode) and 3) sleep fragmentation (SF, 30s ON and 90s OFF mode) group. Six hours later, the animals were tested in the open field, elevated plus maze and light/dark test (n = 8/group). Testosterone, estradiol, progesterone and corticosterone were determined in separate animal cohort immediately upon sleep fragmentation (n = 6/group). RESULTS: SF rats showed decreased rearings number, decreased time spent in the central area and increased thigmotaxic index compared to TC and AC rats in the open field test. Similarly, increased anxiety upon sleep fragmentation was observed in the elevated plus maze and the light/dark test. Significantly lower testosterone, estradiol and progesterone levels were determined in SF in comparison to AC and TC groups, while there was no significant difference in the levels of corticosterone. CONCLUSION: Short term sleep fragmentation enhances anxiety-related behavior in rats, which could be partly mediated by the observed hormonal changes presented in the current study in form of testosterone, estradiol and progesterone depletion. Public Library of Science 2019-07-03 /pmc/articles/PMC6609147/ /pubmed/31269081 http://dx.doi.org/10.1371/journal.pone.0218920 Text en © 2019 Grubac et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Grubac, Zeljko
Sutulovic, Nikola
Ademovic, Anida
Velimirovic, Milica
Rasic-Markovic, Aleksandra
Macut, Djuro
Petronijevic, Natasa
Stanojlovic, Olivera
Hrncic, Dragan
Short-term sleep fragmentation enhances anxiety-related behavior: The role of hormonal alterations
title Short-term sleep fragmentation enhances anxiety-related behavior: The role of hormonal alterations
title_full Short-term sleep fragmentation enhances anxiety-related behavior: The role of hormonal alterations
title_fullStr Short-term sleep fragmentation enhances anxiety-related behavior: The role of hormonal alterations
title_full_unstemmed Short-term sleep fragmentation enhances anxiety-related behavior: The role of hormonal alterations
title_short Short-term sleep fragmentation enhances anxiety-related behavior: The role of hormonal alterations
title_sort short-term sleep fragmentation enhances anxiety-related behavior: the role of hormonal alterations
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6609147/
https://www.ncbi.nlm.nih.gov/pubmed/31269081
http://dx.doi.org/10.1371/journal.pone.0218920
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