Cargando…
Mutual editing of alternative splicing between breast cancer cells and macrophages
Breast cancer is a highly heterogeneous disease and numerous secreted factors may differentially contribute to a macrophage phenotype whose extensive infiltration is generally regarded as indicative of an unfavorable outcome. How different breast tumor cells and macrophage cells interplay or influen...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6609318/ https://www.ncbi.nlm.nih.gov/pubmed/31233192 http://dx.doi.org/10.3892/or.2019.7200 |
_version_ | 1783432291203153920 |
---|---|
author | Ding, Wanbao Li, Dongdong Zhang, Peixian Shi, Lan Dai, Hui Li, Yan Bao, Xin Wang, Yue Zhang, Honglei Deng, Lei |
author_facet | Ding, Wanbao Li, Dongdong Zhang, Peixian Shi, Lan Dai, Hui Li, Yan Bao, Xin Wang, Yue Zhang, Honglei Deng, Lei |
author_sort | Ding, Wanbao |
collection | PubMed |
description | Breast cancer is a highly heterogeneous disease and numerous secreted factors may differentially contribute to a macrophage phenotype whose extensive infiltration is generally regarded as indicative of an unfavorable outcome. How different breast tumor cells and macrophage cells interplay or influence each other on the alternative splicing (AS) level have not been characterized. Here, we exploited one previous study, which investigated the interplay between macrophages and estrogen receptor-positive (ER(+)) breast cancer and triple-negative breast cancer (TNBC) at the transcriptional level, to investigate the tumor-macrophage crosstalk at the AS level. In the present study, it was demonstrated that biological processes such as DNA damage and DNA repair were significantly affected both in ER(+) breast cancer and TNBC by co-culturing with macrophages, whereas biological pathways altered in macrophages co-cultured with tumor cells depended on the breast cancer type. Specifically, biological processes altered in macrophages co-cultured with ER(+) breast cancer were enriched in RNA processing and translation-related pathways whereas biological processes altered in macrophages co-cultured with TNBC were mainly enriched in protein transport pathways. We also analyzed the sequence features of skip exons among different conditions. In addition, putative splicing factors which were responsible for the altered AS profile in each condition were identified. The findings of the present study revealed significant tumor-macrophage crosstalk at the AS level which may facilitate the development of new therapeutic strategies for cancer. |
format | Online Article Text |
id | pubmed-6609318 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-66093182019-07-23 Mutual editing of alternative splicing between breast cancer cells and macrophages Ding, Wanbao Li, Dongdong Zhang, Peixian Shi, Lan Dai, Hui Li, Yan Bao, Xin Wang, Yue Zhang, Honglei Deng, Lei Oncol Rep Articles Breast cancer is a highly heterogeneous disease and numerous secreted factors may differentially contribute to a macrophage phenotype whose extensive infiltration is generally regarded as indicative of an unfavorable outcome. How different breast tumor cells and macrophage cells interplay or influence each other on the alternative splicing (AS) level have not been characterized. Here, we exploited one previous study, which investigated the interplay between macrophages and estrogen receptor-positive (ER(+)) breast cancer and triple-negative breast cancer (TNBC) at the transcriptional level, to investigate the tumor-macrophage crosstalk at the AS level. In the present study, it was demonstrated that biological processes such as DNA damage and DNA repair were significantly affected both in ER(+) breast cancer and TNBC by co-culturing with macrophages, whereas biological pathways altered in macrophages co-cultured with tumor cells depended on the breast cancer type. Specifically, biological processes altered in macrophages co-cultured with ER(+) breast cancer were enriched in RNA processing and translation-related pathways whereas biological processes altered in macrophages co-cultured with TNBC were mainly enriched in protein transport pathways. We also analyzed the sequence features of skip exons among different conditions. In addition, putative splicing factors which were responsible for the altered AS profile in each condition were identified. The findings of the present study revealed significant tumor-macrophage crosstalk at the AS level which may facilitate the development of new therapeutic strategies for cancer. D.A. Spandidos 2019-08 2019-06-18 /pmc/articles/PMC6609318/ /pubmed/31233192 http://dx.doi.org/10.3892/or.2019.7200 Text en Copyright: © Ding et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Ding, Wanbao Li, Dongdong Zhang, Peixian Shi, Lan Dai, Hui Li, Yan Bao, Xin Wang, Yue Zhang, Honglei Deng, Lei Mutual editing of alternative splicing between breast cancer cells and macrophages |
title | Mutual editing of alternative splicing between breast cancer cells and macrophages |
title_full | Mutual editing of alternative splicing between breast cancer cells and macrophages |
title_fullStr | Mutual editing of alternative splicing between breast cancer cells and macrophages |
title_full_unstemmed | Mutual editing of alternative splicing between breast cancer cells and macrophages |
title_short | Mutual editing of alternative splicing between breast cancer cells and macrophages |
title_sort | mutual editing of alternative splicing between breast cancer cells and macrophages |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6609318/ https://www.ncbi.nlm.nih.gov/pubmed/31233192 http://dx.doi.org/10.3892/or.2019.7200 |
work_keys_str_mv | AT dingwanbao mutualeditingofalternativesplicingbetweenbreastcancercellsandmacrophages AT lidongdong mutualeditingofalternativesplicingbetweenbreastcancercellsandmacrophages AT zhangpeixian mutualeditingofalternativesplicingbetweenbreastcancercellsandmacrophages AT shilan mutualeditingofalternativesplicingbetweenbreastcancercellsandmacrophages AT daihui mutualeditingofalternativesplicingbetweenbreastcancercellsandmacrophages AT liyan mutualeditingofalternativesplicingbetweenbreastcancercellsandmacrophages AT baoxin mutualeditingofalternativesplicingbetweenbreastcancercellsandmacrophages AT wangyue mutualeditingofalternativesplicingbetweenbreastcancercellsandmacrophages AT zhanghonglei mutualeditingofalternativesplicingbetweenbreastcancercellsandmacrophages AT denglei mutualeditingofalternativesplicingbetweenbreastcancercellsandmacrophages |