Imaging neuropeptide release at synapses with a genetically engineered reporter

Research on neuropeptide function has advanced rapidly, yet there is still no spatio-temporally resolved method to measure the release of neuropeptides in vivo. Here we introduce Neuropeptide Release Reporters (NPRRs): novel genetically-encoded sensors with high temporal resolution and genetic speci...

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Detalles Bibliográficos
Autores principales: Ding, Keke, Han, Yifu, Seid, Taylor W, Buser, Christopher, Karigo, Tomomi, Zhang, Shishuo, Dickman, Dion K, Anderson, David J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2019
Materias:
Neuroscience
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6609332/
https://www.ncbi.nlm.nih.gov/pubmed/31241464
http://dx.doi.org/10.7554/eLife.46421
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author Ding, Keke
Han, Yifu
Seid, Taylor W
Buser, Christopher
Karigo, Tomomi
Zhang, Shishuo
Dickman, Dion K
Anderson, David J
author_facet Ding, Keke
Han, Yifu
Seid, Taylor W
Buser, Christopher
Karigo, Tomomi
Zhang, Shishuo
Dickman, Dion K
Anderson, David J
author_sort Ding, Keke
collection PubMed
description Research on neuropeptide function has advanced rapidly, yet there is still no spatio-temporally resolved method to measure the release of neuropeptides in vivo. Here we introduce Neuropeptide Release Reporters (NPRRs): novel genetically-encoded sensors with high temporal resolution and genetic specificity. Using the Drosophila larval neuromuscular junction (NMJ) as a model, we provide evidence that NPRRs recapitulate the trafficking and packaging of native neuropeptides, and report stimulation-evoked neuropeptide release events as real-time changes in fluorescence intensity, with sub-second temporal resolution.
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spelling pubmed-66093322019-07-08 Imaging neuropeptide release at synapses with a genetically engineered reporter Ding, Keke Han, Yifu Seid, Taylor W Buser, Christopher Karigo, Tomomi Zhang, Shishuo Dickman, Dion K Anderson, David J eLife Neuroscience Research on neuropeptide function has advanced rapidly, yet there is still no spatio-temporally resolved method to measure the release of neuropeptides in vivo. Here we introduce Neuropeptide Release Reporters (NPRRs): novel genetically-encoded sensors with high temporal resolution and genetic specificity. Using the Drosophila larval neuromuscular junction (NMJ) as a model, we provide evidence that NPRRs recapitulate the trafficking and packaging of native neuropeptides, and report stimulation-evoked neuropeptide release events as real-time changes in fluorescence intensity, with sub-second temporal resolution. eLife Sciences Publications, Ltd 2019-06-26 /pmc/articles/PMC6609332/ /pubmed/31241464 http://dx.doi.org/10.7554/eLife.46421 Text en © 2019, Ding et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Neuroscience
Ding, Keke
Han, Yifu
Seid, Taylor W
Buser, Christopher
Karigo, Tomomi
Zhang, Shishuo
Dickman, Dion K
Anderson, David J
Imaging neuropeptide release at synapses with a genetically engineered reporter
title Imaging neuropeptide release at synapses with a genetically engineered reporter
title_full Imaging neuropeptide release at synapses with a genetically engineered reporter
title_fullStr Imaging neuropeptide release at synapses with a genetically engineered reporter
title_full_unstemmed Imaging neuropeptide release at synapses with a genetically engineered reporter
title_short Imaging neuropeptide release at synapses with a genetically engineered reporter
title_sort imaging neuropeptide release at synapses with a genetically engineered reporter
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6609332/
https://www.ncbi.nlm.nih.gov/pubmed/31241464
http://dx.doi.org/10.7554/eLife.46421
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