Cargando…

Nearly half of Ultrio plus NAT non-discriminated reactive blood donors were identified as occult HBV infection in South China

BACKGROUND: Blood donor plasma samples were detected by the Ultrio Plus NAT system for HBV, HCV and HIV-1 in Shenzhen blood center, China. Reactive samples underwent further discriminatory testing of a single virus by the same methodology. A large number of cases of non-discriminated reactive (NDR)...

Descripción completa

Detalles Bibliográficos
Autores principales: Ye, Xianlin, Li, Tong, Shao, Wen, Zeng, Jinfeng, Hong, Wenxu, Lu, Liang, Zhu, Weigang, Li, Chengyao, Li, Tingting
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6609378/
https://www.ncbi.nlm.nih.gov/pubmed/31269905
http://dx.doi.org/10.1186/s12879-019-4215-9
_version_ 1783432305411358720
author Ye, Xianlin
Li, Tong
Shao, Wen
Zeng, Jinfeng
Hong, Wenxu
Lu, Liang
Zhu, Weigang
Li, Chengyao
Li, Tingting
author_facet Ye, Xianlin
Li, Tong
Shao, Wen
Zeng, Jinfeng
Hong, Wenxu
Lu, Liang
Zhu, Weigang
Li, Chengyao
Li, Tingting
author_sort Ye, Xianlin
collection PubMed
description BACKGROUND: Blood donor plasma samples were detected by the Ultrio Plus NAT system for HBV, HCV and HIV-1 in Shenzhen blood center, China. Reactive samples underwent further discriminatory testing of a single virus by the same methodology. A large number of cases of non-discriminated reactive (NDR) donors were found, leaving potential risk of transmitting HBV if not deferrals. This study identified those non-discriminated samples. METHODS: The NDR plasma samples from blood donation screening were detected and classified by additional molecular and serological tests. Molecular characterizations of DNA+ NDR were determined by sequencing analysis. RESULTS: A number of 259 (0.21%) NDR plasma samples from screening of 123,280 eligible blood donors were detected, which presented a higher rate (91.1%) of anti-HBc reactivity and nearly half (46.7%) of HBV DNA+ that classified as occult HBV infection (OBI). Most OBI strains were wild-type HBV, but some substitutions V168A, S174 N, V177A, Q129R/L/H, G145A/R in S region of genotype B (OBI(B)) and T47K/V/A, P49H/L, Q101R/H/K, S174 N, L175S, V177A, T118 M/R/K, G145R/A/K/E, R160K/N in S region of genotype C (OBI(C)) strains were identified in high frequency. CONCLUSION: Nearly half of NDR blood samples were identified as OBI, in which a number of important mutations were detected. NDR donation might have potential risk for HBV transmission, but need to be further investigated.
format Online
Article
Text
id pubmed-6609378
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-66093782019-07-16 Nearly half of Ultrio plus NAT non-discriminated reactive blood donors were identified as occult HBV infection in South China Ye, Xianlin Li, Tong Shao, Wen Zeng, Jinfeng Hong, Wenxu Lu, Liang Zhu, Weigang Li, Chengyao Li, Tingting BMC Infect Dis Research Article BACKGROUND: Blood donor plasma samples were detected by the Ultrio Plus NAT system for HBV, HCV and HIV-1 in Shenzhen blood center, China. Reactive samples underwent further discriminatory testing of a single virus by the same methodology. A large number of cases of non-discriminated reactive (NDR) donors were found, leaving potential risk of transmitting HBV if not deferrals. This study identified those non-discriminated samples. METHODS: The NDR plasma samples from blood donation screening were detected and classified by additional molecular and serological tests. Molecular characterizations of DNA+ NDR were determined by sequencing analysis. RESULTS: A number of 259 (0.21%) NDR plasma samples from screening of 123,280 eligible blood donors were detected, which presented a higher rate (91.1%) of anti-HBc reactivity and nearly half (46.7%) of HBV DNA+ that classified as occult HBV infection (OBI). Most OBI strains were wild-type HBV, but some substitutions V168A, S174 N, V177A, Q129R/L/H, G145A/R in S region of genotype B (OBI(B)) and T47K/V/A, P49H/L, Q101R/H/K, S174 N, L175S, V177A, T118 M/R/K, G145R/A/K/E, R160K/N in S region of genotype C (OBI(C)) strains were identified in high frequency. CONCLUSION: Nearly half of NDR blood samples were identified as OBI, in which a number of important mutations were detected. NDR donation might have potential risk for HBV transmission, but need to be further investigated. BioMed Central 2019-07-03 /pmc/articles/PMC6609378/ /pubmed/31269905 http://dx.doi.org/10.1186/s12879-019-4215-9 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Ye, Xianlin
Li, Tong
Shao, Wen
Zeng, Jinfeng
Hong, Wenxu
Lu, Liang
Zhu, Weigang
Li, Chengyao
Li, Tingting
Nearly half of Ultrio plus NAT non-discriminated reactive blood donors were identified as occult HBV infection in South China
title Nearly half of Ultrio plus NAT non-discriminated reactive blood donors were identified as occult HBV infection in South China
title_full Nearly half of Ultrio plus NAT non-discriminated reactive blood donors were identified as occult HBV infection in South China
title_fullStr Nearly half of Ultrio plus NAT non-discriminated reactive blood donors were identified as occult HBV infection in South China
title_full_unstemmed Nearly half of Ultrio plus NAT non-discriminated reactive blood donors were identified as occult HBV infection in South China
title_short Nearly half of Ultrio plus NAT non-discriminated reactive blood donors were identified as occult HBV infection in South China
title_sort nearly half of ultrio plus nat non-discriminated reactive blood donors were identified as occult hbv infection in south china
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6609378/
https://www.ncbi.nlm.nih.gov/pubmed/31269905
http://dx.doi.org/10.1186/s12879-019-4215-9
work_keys_str_mv AT yexianlin nearlyhalfofultrioplusnatnondiscriminatedreactiveblooddonorswereidentifiedasocculthbvinfectioninsouthchina
AT litong nearlyhalfofultrioplusnatnondiscriminatedreactiveblooddonorswereidentifiedasocculthbvinfectioninsouthchina
AT shaowen nearlyhalfofultrioplusnatnondiscriminatedreactiveblooddonorswereidentifiedasocculthbvinfectioninsouthchina
AT zengjinfeng nearlyhalfofultrioplusnatnondiscriminatedreactiveblooddonorswereidentifiedasocculthbvinfectioninsouthchina
AT hongwenxu nearlyhalfofultrioplusnatnondiscriminatedreactiveblooddonorswereidentifiedasocculthbvinfectioninsouthchina
AT luliang nearlyhalfofultrioplusnatnondiscriminatedreactiveblooddonorswereidentifiedasocculthbvinfectioninsouthchina
AT zhuweigang nearlyhalfofultrioplusnatnondiscriminatedreactiveblooddonorswereidentifiedasocculthbvinfectioninsouthchina
AT lichengyao nearlyhalfofultrioplusnatnondiscriminatedreactiveblooddonorswereidentifiedasocculthbvinfectioninsouthchina
AT litingting nearlyhalfofultrioplusnatnondiscriminatedreactiveblooddonorswereidentifiedasocculthbvinfectioninsouthchina