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Recombinant yeast VDAC2: a comparison of electrophysiological features with the native form
Voltage‐dependent anion channel isoform 2 of the yeast Saccharomyces cerevisiae (yVDAC2) was believed for many years to be devoid of channel activity. Recently, we isolated yVDAC2 and showed that it exhibits channel‐forming activity in the planar lipid bilayer system when in its so‐called native for...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6609568/ https://www.ncbi.nlm.nih.gov/pubmed/31206247 http://dx.doi.org/10.1002/2211-5463.12574 |
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author | Magrì, Andrea Karachitos, Andonis Di Rosa, Maria Carmela Reina, Simona Conti Nibali, Stefano Messina, Angela Kmita, Hanna De Pinto, Vito |
author_facet | Magrì, Andrea Karachitos, Andonis Di Rosa, Maria Carmela Reina, Simona Conti Nibali, Stefano Messina, Angela Kmita, Hanna De Pinto, Vito |
author_sort | Magrì, Andrea |
collection | PubMed |
description | Voltage‐dependent anion channel isoform 2 of the yeast Saccharomyces cerevisiae (yVDAC2) was believed for many years to be devoid of channel activity. Recently, we isolated yVDAC2 and showed that it exhibits channel‐forming activity in the planar lipid bilayer system when in its so‐called native form. Here, we describe an alternative strategy for yVDAC2 isolation, through heterologous expression in bacteria and refolding in vitro. Recombinant yVDAC2, like its native form, is able to form voltage‐dependent channels. However, some differences between native and recombinant yVDAC2 emerged in terms of voltage dependence and ion selectivity, suggesting that, in this specific case, the recombinant protein might be depleted of post‐translational modification(s) that occur in eukaryotic cells. |
format | Online Article Text |
id | pubmed-6609568 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-66095682019-07-16 Recombinant yeast VDAC2: a comparison of electrophysiological features with the native form Magrì, Andrea Karachitos, Andonis Di Rosa, Maria Carmela Reina, Simona Conti Nibali, Stefano Messina, Angela Kmita, Hanna De Pinto, Vito FEBS Open Bio Research Articles Voltage‐dependent anion channel isoform 2 of the yeast Saccharomyces cerevisiae (yVDAC2) was believed for many years to be devoid of channel activity. Recently, we isolated yVDAC2 and showed that it exhibits channel‐forming activity in the planar lipid bilayer system when in its so‐called native form. Here, we describe an alternative strategy for yVDAC2 isolation, through heterologous expression in bacteria and refolding in vitro. Recombinant yVDAC2, like its native form, is able to form voltage‐dependent channels. However, some differences between native and recombinant yVDAC2 emerged in terms of voltage dependence and ion selectivity, suggesting that, in this specific case, the recombinant protein might be depleted of post‐translational modification(s) that occur in eukaryotic cells. John Wiley and Sons Inc. 2019-06-17 /pmc/articles/PMC6609568/ /pubmed/31206247 http://dx.doi.org/10.1002/2211-5463.12574 Text en © 2018 The Authors. Published by FEBS Press and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Magrì, Andrea Karachitos, Andonis Di Rosa, Maria Carmela Reina, Simona Conti Nibali, Stefano Messina, Angela Kmita, Hanna De Pinto, Vito Recombinant yeast VDAC2: a comparison of electrophysiological features with the native form |
title | Recombinant yeast VDAC2: a comparison of electrophysiological features with the native form |
title_full | Recombinant yeast VDAC2: a comparison of electrophysiological features with the native form |
title_fullStr | Recombinant yeast VDAC2: a comparison of electrophysiological features with the native form |
title_full_unstemmed | Recombinant yeast VDAC2: a comparison of electrophysiological features with the native form |
title_short | Recombinant yeast VDAC2: a comparison of electrophysiological features with the native form |
title_sort | recombinant yeast vdac2: a comparison of electrophysiological features with the native form |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6609568/ https://www.ncbi.nlm.nih.gov/pubmed/31206247 http://dx.doi.org/10.1002/2211-5463.12574 |
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