Rapid MALDI mass spectrometry imaging for surgical pathology

Matrix assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) is an emerging analytical technique, which generates spatially resolved proteomic and metabolomic images from tissue specimens. Conventional MALDI MSI processing and data acquisition can take over 30 min, limiting its...

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Detalles Bibliográficos
Autores principales: Basu, Sankha S., Regan, Michael S., Randall, Elizabeth C., Abdelmoula, Walid M., Clark, Amanda R., Gimenez-Cassina Lopez, Begoña, Cornett, Dale S., Haase, Andreas, Santagata, Sandro, Agar, Nathalie Y. R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Brief Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6609678/
https://www.ncbi.nlm.nih.gov/pubmed/31286061
http://dx.doi.org/10.1038/s41698-019-0089-y
Descripción
Sumario:Matrix assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) is an emerging analytical technique, which generates spatially resolved proteomic and metabolomic images from tissue specimens. Conventional MALDI MSI processing and data acquisition can take over 30 min, limiting its clinical utility for intraoperative diagnostics. We present a rapid MALDI MSI method, completed under 5 min, including sample preparation and analysis, providing a workflow compatible with the clinical frozen section procedure.

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